Code | CSB-MA269399 |
Size | US$166 |
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Application | Recommended Dilution |
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IHC | 1:20-1:200 |
To generate the SPN monoclonal antibody, the synthesized peptide derived from human CD43 protein is used to immunize mice. After immunization, B cells are isolated from the mouse and then fuse with myeloma cells to form hybridomas. The hybridoma cells that produce the SPN antibody are selected to culture in the mouse abdominal cavity. The SPN monoclonal antibody is affinity-purified from mouse ascites by affinity-chromatography using a specific immunogen. It can be used for ELISA and IHC applications to recognize human SPN protein.
SPN (sialophorin), also known as CD43 or leukosialin, mainly acts as a negative regulator of cell adhesion by preventing leukocyte adhesion to the endothelium and extracellular matrix proteins. By binding to selectins, CD43 can prevent the binding of other adhesion molecules and thus inhibit leukocyte recruitment to sites of inflammation. In addition to its role in cell adhesion, CD43 has also been implicated in the regulation of lymphocyte activation and differentiation.
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