Product Details

Purity

≥ 85% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human CLDN1 at 2 μg/mL on an Nickel Coated plate can bind Anti-CLDN1 recombinant antibody (CSB-RA005490MA1HU). The EC50 is 62.47-72.10 ng/mL.

Target Names

CLDN1

Uniprot No.

O95832

Alternative Names

Senescence-associated epithelial membrane protein; CLDN1; CLD1, SEMP1; UNQ481/PRO944

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

2-211aa

Target Protein Sequence

ANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV

Mol. Weight

25.1 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-flag tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm sterile filtered 50 mM HEPES, 0.15 M NaCl, 0.05% DDM, 0.01% CHS, 6% Trehalose, pH 7.5

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Claudin-1 functions as a critical tight junction protein whose dysregulation correlates with epithelial barrier disruption in multiple cancers, making it a valuable target for investigating tumor progression and metastatic mechanisms. This full-length construct spanning residues 2–211 demonstrates quantifiable antibody-binding activity with an EC50 of 62.47–72.10 ng/mL in functional ELISA, confirming that the protein retains native epithelial conformational epitopes despite detergent solubilization. Mammalian expression preserves the post-translational modifications essential for authentic tight junction protein folding, providing a suitable basis for antibody development campaigns, epitope mapping studies, and binding competition assays that require physiologically relevant target presentation. The combination of greater than 85% purity and endotoxin levels below 1.0 EU/μg aligns with standards expected in cell-based barrier function assays and protein-protein interaction screens where contaminant interference would compromise experimental interpretation.

Target Background

Function

Claudins function as major constituents of the tight junction complexes that regulate the permeability of epithelia. While some claudin family members play essential roles in the formation of impermeable barriers, others mediate the permeability to ions and small molecules. Often, several claudin family members are coexpressed and interact with each other, and this determines the overall permeability. CLDN1 is required to prevent the paracellular diffusion of small molecules through tight junctions in the epidermis and is required for the normal barrier function of the skin. Required for normal water homeostasis and to prevent excessive water loss through the skin, probably via an indirect effect on the expression levels of other proteins, since CLDN1 itself seems to be dispensable for water barrier formation in keratinocyte tight junctions.; (Microbial infection) Acts as a co-receptor for hepatitis C virus (HCV) in hepatocytes. Associates with CD81 and the CLDN1-CD81 receptor complex is essential for HCV entry into host cell. Acts as a receptor for dengue virus.

Gene References into Functions

the accumulation and toxicity of doxorubicin were rescued by CLDN1 siRNA in A549R cells. We suggest that CLDN1 is upregulated by CDDP resistance through activation of a PI3K/Akt/NF-kappaB pathway, resulting in the inhibition of penetration of anticancer drugs into the inner area of spheroids PMID: 29524521
Our data indicate that CLDN1 targeting with an anti-CLDN1 mAb results in decreased growth and survival of CRC cells. This suggests that CLDN1 could be a new potential therapeutic target. PMID: 28659146
Human Growth Hormone Inhibits CLAUDIN-1 Expression Through Activation of Signal Transducer and Activator of Transcription 3 (STAT3). PMID: 28617312
CLDN1 promotes invasion and metastasis in cervical cancer cells via the expression of EMT/invasion-related genes. PMID: 27974683
Cycling hypoxia could induce significant changes in CLDN1 and CLDN7 expression in nasopharyngeal cancer cells, indirectly regulation P18 expression and affecting cell invasion/proliferation. PMID: 28055967
Rab25 is amplified and enhances aggressiveness in luminal B cancers while in claudin-low tumors, Rab25 is lost indicating possible anti-tumor functions PMID: 27259233
association of genetic polymorphisms of claudin-1 with small vessel vascular dementia PMID: 28273404
Glutamine increased claudin-1 expression in the colonic mucosa of patients with irritable bowel syndrome. PMID: 25972430
CLDN-1 promoted the migration and EMT through the Notch signaling pathway. PMID: 28316062
In human lung tissue, Claudin-1 is higher in RBFOX3-positive cells than in RBFOX3-negative cells. Immunostaining and mRNA quantification revealed that protein levels, but not mRNA levels, of Claudin-1 are increased by RBFOX3. PMID: 28126724
Data show that miR-30a could bind to the 3'-untranslted region of Slug mRNA and increased expression of claudins, a family of tight junction transmembrane proteins. PMID: 26918943
These findings indicate a previously unrecognized mechanism that miR-142-5p, targeting CLDN1, plays an important role in Hashimoto's thyroiditis pathogenesis. PMID: 27277258
Here, the authors identified an interaction between claudin-1 and Sec24C, a cargo-sorting component of the coat protein complex II (COPII) vesicular transport system. PMID: 28679754
Aberrant expression of the tight junction molecules claudin-1 and zonula occludens-1 mediates cell growth and invasion in oral squamous cell carcinoma cells. PMID: 27436828
Increased expression of intestinal epithelial claudin-1 with downregulation of claudin-3 has been observed in intestinal inflammatory disorders. PMID: 28493289
Studies indicate claudin 1 (CLDN-1) as a target for improving epidermal drug absorption and preventing HCV infection and of claudin 4 (CLDN-4) as a target for anticancer therapeutics. PMID: 28415141
Data show that the charge of Lys65 in claudin 1 (Cldn1) and Glu158 in claudin 3 (Cldn3), and of Gln57 in claudin 5 (Cldn5) are necessary for tight junction (TJ) strand formation. PMID: 28415153
Studies indicate that claudin-1 accumulate in early endosomes in both epithelial and endothelial cells. PMID: 28415156
Claudin-low tumors were associated with young age of onset, higher tumor grade, larger tumor size, extensive lymphocytic infiltrate and a circumscribed tumor margin. Patients with claudin-low tumors had a worse overall survival when compared to patients with luminal A type breast cancer. PMID: 28045912
These results revealed that CLDN1 contributed to cancer stem cell features of hepatocellular carcinoma, which was altered by TMPRSS4 expression via ERK1/2 signaling pathway, providing promising targets for novel specific therapies. PMID: 28651932
Data suggest that epithelial-mesenchymal transition (EMT) is regulated by ZMYND8 (receptor for activated protein kinase C) which selectively activates gene promoters of CLDN1 (claudin 1) and CDH1 (E-cadherin) in breast cancer cells; thus, the presence of ZMYND8 could be implicated in maintaining epithelial phenotype of cells; ZMYND8 regulates invasion/migration of breast cancer cells. PMID: 28432260
The data suggested that miR-29a may regulate tumor growth and migration by targeting CLDN1. PMID: 28342862
TNF-alpha mediated regulation of claudin-1 and tumorigenic abilities of colon cancer cells. PMID: 27742576
CLDN1 is significantly hypomethylated in tumor samples and that the membrane staining intensity for claudin 1, 4, and 7 is significantly lower in colorectal cancer tissues than in adjacent nonneoplastic tissue. PMID: 28381183
CLDN1 rather than variants in FLG may be involved in the susceptibility of AD in the Ethiopian population PMID: 27581203
CLDN1 overexpression is a good prognostic factor in NSCLC. PMID: 27687058
Studies showthat CLDN1 is downregulated in lung adenocarcinoma and that low CLDN1 messenger ribonucleic acid (mRNA) expression leads to shorter overall survival. PMID: 27766775
The loss of claudin-1 appears to be involved in the pathogenesis of pterygium PMID: 26820697
autotypic tight junctions molecular composition, like claudin-1 and occludin expression could influence the demyelinating process by altering the permeability of the blood-nerve barrier. PMID: 26662145
High CLDN1 expression is associated with Cervical cancer. PMID: 26463354
Claudin-1 expression was correlated with lymphatic microvessel generation in hypopharyngeal squamous cell carcinoma and with patient survival. PMID: 26436506
High CLAUDIN-1 expression is associated with metastasis in follicular thyroid carcinoma. PMID: 26219679
The highest expression of claudin-1 was observed in well-differentiated oral squamous cell carcinomas, whereas poorly differentiated tumors exhibited mostly no expression of claudin-1. PMID: 25517868
Report genetic polymorphism of TJ component claudin-1 and their haplotypes are associated with leukoaraiosis. PMID: 25956626
Using a human liver-chimeric mouse model, we show that a monoclonal antibody specific for the TJ protein claudin-1 (ref. 7) eliminates chronic HCV infection without detectable toxicity PMID: 25798937
delocalization of claudin-1 induced by PKC phosphorylation contributes to metastatic capacity of osteosarcoma cells PMID: 26361141
The expressions of MARVELD2, CLDN1 and CLDN3 mRNA were significantly lower in cholesteatoma tissue and may be involved in epithelium permeability. PMID: 25319490
There was no difference in the frequency of fractures or renal lithiasis between the rs219780 genotype groups in PHPT patients. Nor was there any relationship between the T or C alleles and BMD or biochemical parameters. PMID: 26554238
CLD1-mediated hepatoma cell invasiveness occurs via heat shock factor 1 (HSF1) activation. PMID: 26157141
Findings suggest that CLDN1 is oncogenic in gastric cancer and its malignant potential may be attributed in part to regulation of anoikis, by mediating membrane beta-catenin-regulated cell-cell adhesion and cell survival. PMID: 25544763
reduction of claudin-1 expression may contribute to the progression of gastric tumors PMID: 25755790
Data from live-cell imaging suggest at least two different cis-interaction interfaces within CLDN3 homopolymers as well as within CLDN1/CLDN3 heteropolymers. PMID: 25849148
Suggest that claudin 1 is an important messenger that regulates TNF-alpha-induced gene expression and migration in gastric cancer cells. PMID: 25548484
The findings suggest that down regulated CLDN1 and CLDN5 genes have potential relevance in relation to the progression of glioblastoma multiforme. PMID: 25345514
Positive expressions of Claudin-1 in retinoblastoma were fewer than those in retina; higher positive expressions were found in differentiated tissues than in undifferentiated tissues; Claudin-1 expressed more positively in optic nerves without invasion. PMID: 26122225
findings suggest that homozygous CD81 rs708564 TT may be a genetic modifier for avoiding HCV infection whether as a sole single nucleotide polymorphism or combined with the CLDN1 rs893051 GG genotype PMID: 25934191
Our results indicate that CLDN4 expression is correlated with poor prognosis, and CLDN1 expression may be an indicator of recurrence of oral squamous cell carcinoma. PMID: 25964581
CLDN1-negative phenotype predicts a high risk of recurrence and death in triple-negative breast cancer. PMID: 25393310
dystopic subcellular localizations of Snail-1 and claudin-1 may participate in changes of cellular morphology and behavior which might be associated with altered effectory pathways of proteins and thus substantially contribute to the cancer development. PMID: 22408413
Data show that C-terminal domain of Clostridium perfringens enterotoxin (cCPE)-binding to claudin 1 and claudin 5 is prevented by two residues in extracellular loop 2 of Cld1 (Asn(150) and Thr(153)) and Cld5 (Asp(149) and Thr(151)). PMID: 25342221

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Involvement in disease

Ichthyosis-sclerosing cholangitis neonatal syndrome (NISCH)

Subcellular Location

Cell junction, tight junction. Cell membrane; Multi-pass membrane protein. Basolateral cell membrane.

Protein Families

Claudin family

Tissue Specificity

Strongly expressed in liver and kidney. Expressed in heart, brain, spleen, lung and testis.

Database Links

HGNC: 2032

OMIM: 603718

KEGG: hsa:9076

STRING: 9606.ENSP00000295522

UniGene: Hs.439060

Code	CSB-MP005490HU1-D
Abbreviation	Recombinant Human CLDN1 protein-Detergent (Active)
MSDS	MSDS Datasheet
Size	$725
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human CLDN1 at 2 μg/ml on an Nickel Coated plate can bind Anti-CLDN1 recombinant antibody (CSB-RA005490MA1HU). The EC₅₀ is 62.47-72.10 ng/mL. Biological Activity Assay
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Recombinant Human Claudin-1 (CLDN1)-Detergent (Active)

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