Product Details

Purity

≥ 95% as determined by SDS-PAGE.

Endotoxin

≤10 EU/mg by the LAL method

Activity

Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED50 for this effect is ≤2.0 ng/mL.

Target Names

IL-18

Uniprot No.

Q14116

Alternative Names

Interleukin-18; Iboctadekin; Interferon gamma-inducing factor; IFN-gamma-inducing factor; Interleukin-1 gamma; IL-1 gamma;GIF; IL-18; IL-1g; IL1F4; MGC12320

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

37-193aa

Target Protein Sequence

YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

Mol. Weight

18.2 kDa

Protein Length

Full Length of Mature Protein

Tag Info

Tag free

Form

Lyophilized
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered solution containing PBS, 5% mannitoland 0.01% Tween 80, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

IL-18 plays a central role in Th1 polarization by synergizing with IL-12 to drive IFN-γ production, yet its activity is notoriously sensitive to endotoxin contamination that can confound immune cell readouts. This tag-free recombinant human IL-18, spanning the full mature protein sequence (aa 37–193), demonstrates potent bioactivity with an ED50 ≤2.0 ng/mL in inducing IFN-γ secretion by KG-1 cells co-stimulated with TNF-α, providing a quantitative benchmark for dose-response studies in Th1 differentiation and macrophage activation assays. Endotoxin levels ≤10 EU/mg satisfy the stringent criteria expected for primary immune cell cultures, PBMC-based functional assays, and in vivo inflammation models where LPS artifacts must be minimized. Purity exceeding 95% by SDS-PAGE, combined with the validated signaling potency, supports use in JAK/STAT and NF-κB pathway analysis, as well as antibody development workflows requiring a functional immunogen or ELISA standard.

Code	CSB-MP614514HU-WD
Abbreviation	Recombinant Human IL-18 protein (Active)
MSDS	MSDS Datasheet
Size	Inquiry
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Interleukin-18 (IL18) (Active)

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