Name: Recombinant Human Leukemia inhibitory factor receptor (LIFR), partial (Active)
Brand: CUSABIO
SKU: CSB-MP012929HUi9

Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized human LIF (CSB-MP012928HU) at 2 μg/ml can bind human LIFR, the EC₅₀ is 11.68-15.52 ng/ml.

Target Names

LIFR

Uniprot No.

P42702

Alternative Names

(LIF receptor)(LIF-R)(CD118)

Molecular Characterization

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

45-833aa

Target Protein Sequence

QKKGAPHDLKCVTNNLQVWNCSWKAPSGTGRGTDYEVCIENRSRSCYQLEKTSIKIPALSHGDYEITINSLHDFGSSTSKFTLNEQNVSLIPDTPEILNLSADFSTSTLYLKWNDRGSVFPHRSNVIWEIKVLRKESMELVKLVTHNTTLNGKDTLHHWSWASDMPLECAIHFVEIRCYIDNLHFSGLEEWSDWSPVKNISWIPDSQTKVFPQDKVILVGSDITFCCVSQEKVLSALIGHTNCPLIHLDGENVAIKIRNISVSASSGTNVVFTTEDNIFGTVIFAGYPPDTPQQLNCETHDLKEIICSWNPGRVTALVGPRATSYTLVESFSGKYVRLKRAEAPTNESYQLLFQMLPNQEIYNFTLNAHNPLGRSQSTILVNITEKVYPHTPTSFKVKDINSTAVKLSWHLPGNFAKINFLCEIEIKKSNSVQEQRNVTIKGVENSSYLVALDKLNPYTLYTFRIRCSTETFWKWSKWSNKKQHLTTEASPSKGPDTWREWSSDGKNLIIYWKPLPINEANGKILSYNVSCSSDEETQSLSEIPDPQHKAEIRLDKNDYIISVVAKNSVGSSPPSKIASMEIPNDDLKIEQVVGMGKGILLTWHYDPNMTCDYVIKWCNSSRSEPCLMDWRKVPSNSTETVIESDEFRPGIRYNFFLYGCRNQGYQLLRSMIGYIEELAPIVAPNFTVEDTSADSILVKWEDIPVEELRGFLRGYLFYFGKGERDTSKMRVLESGRSDIKVKNITDISQKTLRIADLQGKTSYHLVLRAYTDGGVGPEKSMYVVTKENS

Mol. Weight

119.3 kDa

Protein Length

Partial

Tag Info

C-terminal hFc-Flag-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

A part of human LIFR (45-833AA) carrying a C-terminal hFc-Flag tag was expressed in mammalian cells. The product is the recombinant human LIFR protein. It is an active protein, whose bioactivity has been validated in a functional ELISA by binding to the human LIF with the EC₅₀ of 11.68-15.52 ng/ml. Its purity is greater than 95% determined by SDS-PAGE. It contains less than 1.0 EU/ug measured by the LAL method. It is in stock now so that there is no preparation waiting period.

LIFR is a heterodimeric receptor consisting of LIFRβ and gp130 subunits. LIFR/LIF interaction is involved in the proliferation, differentiation, as well as development and progression of cancers.

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

Submit a Review here

Applications : Antigen, Binding assay/Protein-protein interaction

Review: We used CSB-MP012929HUi9 product to carry out functional ELISA experiments, coated with human LIF recombinant protein (CSB-MP012928HUd9), which can bind LIFR at 2 μg/ml, with EC50 of 22.58-30.24 ng/ml, which proves that the recombinant protein has better binding activity. Subsequently, it was used as an antigen for immunization, the mouse serum titer was high, and the screened antibodies could better recognize my sample. It proves that the antigen has complete biological activity and is close to the structure of a natural sample. The entire experiment progressed smoothly. I plan to continue purchasing in large quantities!

By Anonymous

Read more reviews >>

Target Background

Function

Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells.

Gene References into Functions

High levels of LIFR in colorectal cancer (CRC) facilitated proliferation and migration of endothelial cells, resulting in an increase in angiogenic activity. Moreover, IL-8 was found to play a role in the LIFR induced angiogenesis. IL-8 levels were correlated with LIFR levels in CRC tissues, whereas depletion of IL-8 led to a reduced angiogenic activity of LIFR in CRC cells. PMID: 29751081
These data indicate that miR-377-3p suppressed adipogenesis of human bone marrow mesenchymal stem cells by targeting LIFR, which provides novel insights into the molecular mechanism of miRNA-mediated cellular differentiation. PMID: 29959592
High expression of LIFR is associated with preeclampsia. PMID: 29363569
LIFR attenuates tumor metastasis by suppressing YAP expression, suggesting that LIFR may serve as a potential target for clear cell renal cell carcinoma treatment. PMID: 29902078
Data show that lncRNA-CTD-2108O9.1 represses metastasis by targeting leukemia inhibitory factor receptor (LIFR). PMID: 29603493
Significant reduction in LIFR expression and the reduced activation of subsequent signaling strongly suggest a working model of how the implantation markers, LIF, may affect the endometrium of patients with adenomyosis. PMID: 27903796
Expression of cancer-specific glycan epitopes represents an excellent opportunity for diagnostics and potentially specific detection of tumors. Here, we report four proteins (LIFR, CE350, VP13A, HPT) found in sera from pancreatic cancer patients carrying aberrant glycan structures as compared to those of controls. PMID: 28244758
Authors demonstrate heterozygous novel or rare LIFR mutations in 3.3% of CAKUT patients, and provide evidence that Lifr deficiency and deactivating LIFR mutations cause highly similar anomalies of the urogenital tract in mice and humans. PMID: 28334964
LIFR inhibited the expression of beta-catenin. PMID: 27375070
The Leukemia Inhibitory Factor Receptor Gene Is a Direct Target of RUNX1 PMID: 26060100
We report the case of a girl with Stuve-Wiedemann syndrome confirmed on molecular analysis. PMID: 25868946
The C65S mutant LIFR showed altered glycosylation and an elevated expression level that might be attributed to a slow turnover of the mutant form. PMID: 26285796
High LIFr expression stimulates melanoma cell migration and is associated with unfavorable prognosis in melanoma. PMID: 26329521
Stuve-Wiedemann syndrome patients with (p.Arg692X) LIFR mutation may develop central adrenal insufficiency due to impaired LIF/LIFR signalling. LIF/LIFR system plays a role in human HPA axis regulation. PMID: 25145448
these findings conclude that LIFR functions as a novel metastasis suppressor in Hepatocellular carcinoma and may serve as a prognostic biomarker for Hepatocellular carcinoma patients. PMID: 26249360
LIFR may play a functional role in hepatocarcinogenesis PMID: 25749520
Expression of the LIF receptor was strongly increased on immune cells of multiple sclerosis patients. PMID: 25514345
The LIFRalpha-CT3 TAT fusion protein can inhibit miR-155 expression PMID: 25092123
LIFR signaling usually follows the JAK/STAT3 pathway, and is initiated by several interleukin-6-type cytokines PMID: 24618404
R28E mutation in CNTF abrogatesIL-6 receptor-dependent but retains CNTF receptor-dependent signaling via glycoprotein 130/ LIFR. PMID: 24802752
LIFR rs3729740 and possibly ANXA11 rs1049550 may be useful as biomarkers for predicting whether metastatic colorectal cancer patients are sensitive to relevant target regimens, although further validation in large cohorts is needed. PMID: 23579219
Because acute ventricle enlargement is observed in susceptible CD118-deficient mice, the phenomenon may occur in a subpopulation of human adults with herpes simplex encephalitis. PMID: 23382563
findings show that LIFR is a key novel tumor suppressor, whose deregulation may drive the transformation of a significant proportion of human breast cancers PMID: 22535017
findings identify LIFR as a metastasis suppressor that functions through the Hippo-YAP pathway and has significant prognostic power. PMID: 23001183
A unique loop structure in oncostatin M determines binding affinity toward oncostatin M receptor and leukemia inhibitory factor receptor. PMID: 22829597
A significant association was detected between the LIFR gene polymorphisms and schizophrenia patients with persecutory delusion PMID: 21971603
LIF and LIFR immunolocalized to decidual cells in the mid-late secretory phase endometrium and 1st trimester decidua. PMID: 21966484
These results demonstrate that cancer-specific methylation and a specific decrease of LIFR expression are a common inactivation event in colon cancer development. PMID: 21617854
Down regulation of Leukemia inhibitory factor receptor is associated with hepatocellular carcinoma. PMID: 19733004
The present study indicated that the LIF gene variant may produce susceptibility to hebephrenic schizophrenia and deterioration of working memory function. PMID: 19879916
Review. The structure and function of the LIF-R gene and protein, mRNA processing, and its role in tumor cells are reviewed. PMID: 11042511
upper cytokine-binding module and the Ig-like domain of the leukaemia inhibitory factor (LIF) receptor are sufficient for a functional LIF receptor complex PMID: 11812136
Separate functions for the two modules of the membrane-proximal cytokine binding domain of glycoprotein 190, the leukemia inhibitory factor low affinity receptor, in ligand binding and receptor activation (gp190) PMID: 11834739
interactions of CNTFR with LIFR and gp130 in vitro PMID: 12707266
mutations alter the stability of LIFR messenger RNA transcripts, resulting in the absence of the LIFR protein and in the impairment of the JAK/STAT3 signaling pathway in patient cells PMID: 14740318
immunocytochemical staining and mRNA expression of LIF and its receptor are consistent with the concept that LIF might be involved in growth initiation of human primordial follicles through its receptor PMID: 15044601
In cells overexpressing a LIFR mutant with the N-terminal cytokine binding domain deleted, signaling by ciliary neurotrophic factor was abolished. PMID: 16051226
Expression of leukemia inhibitory factor and its receptor is low in undifferentiated human embryonic stem cells but increases during differentiation. PMID: 16949591
sOSMR is able to bind OSM and interleukin-31 when associated to soluble gp130 or soluble interleukin-31R, respectively, and to neutralize both cytokine properties PMID: 17028186
Results present the biophysical and structural characterization of the full-length, transmembrane form of a quaternary cytokine receptor complex consisting of gp130, LIF-R, Ciliary Neurotrophic Factor (CNTF), and its alpha receptor (CNTF-Ralpha). PMID: 18775332

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Involvement in disease

Stueve-Wiedemann syndrome (STWS)

Subcellular Location

[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.

Protein Families

Type I cytokine receptor family, Type 2 subfamily

Database Links

HGNC: 6597

OMIM: 151443

KEGG: hsa:3977

STRING: 9606.ENSP00000263409

UniGene: Hs.133421

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