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A DNA fragment encoding amino acid residues Phe19 to Lys379 of the human TNFRSF8 was fused with a Myc-tag at the C-terminus and a 10xHis tag at the N-terminus and then expressed in mammalian cells. The product is the recombinant human TNFRSF8 protein. Its purity is greater than 95% determined by SDS-PAGE. Under reducing conditions, the TNFRSF8 protein migrated to the molecular mass band of approximately 66 kDa on the gel. Its endotoxin content is less than 1.0 EU/ug measured by the LAL method. It is an active protein, and its bioactivity was measured through the functional ELISA. It can bind to CD30L with the EC50 of 14.96-20.25 ng/ml. This recombinant TNFRSF8 protein is in stock now.
TNFRSF8, also called CD30, is normally expressed by activated T- and B-lymphocytes and NK cells. Upon binding to CD30L, CD30 may participate in Th1 and Th2 cell responses and plays a key role in Th17 differentiation and Th1-, and Th2-related diseases. Upregulation of CD30 expression is found in various hematological malignancies, including anaplastic large cell lymphoma (ALCL) and subsets of Non-Hodgkin’s lymphomas (NHLs).
Applications : Binding assay/Protein-protein interaction
Review: After receiving the product, we performed SDS-PAGE detection and found that the purity was higher than the standard. The binding activity between TNFRSF8 (CD30) and CD30L was verified by a functional ELISA to be very good, and the EC50 was 9.531-12.49 ng/ml. The protein has stable performance, good repeatability, and small inter-batch differences, which can be used for subsequent research! The product is easy to use and worth being recommended!