Purity

≥ 90% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CLDN18 at 5 μg/ml can bind Anti-CLDN18.2 recombinant Antibody (CSB-RA005498A1HU), the EC₅₀ is 2.806-3.641 ng/ml.

Target Names

CLDN18.2

Uniprot No.

A0A2K5VV62

Molecular Characterization

Species

Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Source

Mammalian cell

Expression Region

1-261aa

Target Protein Sequence

MAVTACQGLGFVVSLIGIAGIIAATCMDQWSTQDLYNNPVTAVFNYQGLWRSCVRESSGFTECRGYFTLLGLPAMLQAVRALMIVGIVLGAIGLLVSIFALKCIRIGSMEDSAKANMTLTSGIMFIVSGLCAIAGVSVFANMLVTNFWMSTANMYTGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLAPEETNYKAVSYHASGHSVAYKPGGFKASTGFGSNTKNKKTYDGGAHTEDELQSYPSKHDYV

Mol. Weight

28.7 kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-tagged (This tag can be tested only under denaturing conditions)

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Claudin 18.2 has emerged as a high-priority therapeutic target in oncology due to its restricted expression pattern and surface accessibility on tumor cells, yet its four-transmembrane topology makes soluble recombinant production notoriously difficult. This Macaca fascicularis CLDN18.2 construct (full-length, aa 1–261) overcomes that challenge by presenting the protein in virus-like particles (VLPs) derived from mammalian cells, preserving native conformation and extracellular loop architecture critical for antibody epitope recognition. Functional ELISA confirms robust binding to an anti-CLDN18.2 recombinant antibody (CSB-RA005498A1HU) with an EC50 of 2.806–3.641 ng/ml, directly supporting antibody development, cross-reactivity screening against cynomolgus orthologs, and validation of therapeutic candidates targeting CLDN18.2 in tumor metastasis research. The VLP format, combined with endotoxin levels below 1.0 EU/μg, provides a suitable basis for immunization protocols and cell-based binding assays where conformational integrity is essential.

Code	CSB-MP4304MOV
Abbreviation	Recombinant Cynomolgus monkey CLDN18.2 protein-VLPs (Active)
MSDS	MSDS Datasheet
Size	$620
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Image	CSB-MP4304MOV is detected by Mouse anti-6*His monoclonal antibody. Activity Measured by its binding ability in a functional ELISA. Immobilized Macaca fascicularis CLDN18 at 5 μg/ml can bind Anti-CLDN18.2 recombinant Antibody (CSB-RA005498A1HU), the EC₅₀ is 2.806-3.641 ng/ml. Biological Activity Assay The purity of VLPs was greater than 90% as determined by SEC-HPLC
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Recombinant Macaca fascicularis Claudin 18.2 (CLDN18.2)-VLPs (Active)

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