E2F1 Antibody

Datasheet
Code CSB-PA007340LA01HU
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Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. Q01094
Image
  • Western blot
    All lanes: E2F1 antibody at 7 μg/ml + 3T3 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 47 kDa
    Observed band size: 47 kDa

  • Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA007340LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA007340LA01HU at dilution of 1:100

  • Immunoprecipitating E2F1 in 3T3 whole cell lysate
    Lane 1: Rabbit control IgG (1μg) instead of CSB-PA007340LA01HU in 3T3 whole cell lysate. For western blotting, a HRP-conjugated light chain specific antibody was used as the secondary antibody (1/50000)
    Lane 2: CSB-PA007340LA01HU (8μg) + 3T3 whole cell lysate (500μg)
    Lane 3: 3T3 whole cell lysate (10μg)

Immunogen Recombinant Human Transcription factor E2F1 protein (133-362AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IP; Recommended dilution: WB:1:500-1:5000, IHC:1:20-1:200, IP:1:200-1:2000
Relevance Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Transcription factor E2F1 (E2F-1) (PBR3) (Retinoblastoma-associated protein 1) (RBAP-1) (Retinoblastoma-binding protein 3) (RBBP-3) (pRB-binding protein E2F-1), E2F1, RBBP3
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunoprecipitation (IP) Protocol
Research Area Epigenetics and Nuclear Signaling
Target Names E2F1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters
Subcellular Location Nucleus
Protein Families E2F/DP family
Database Links

HGNC: 3113

OMIM: 189971

KEGG: hsa:1869

STRING: 9606.ENSP00000345571

UniGene: Hs.654393

Pathway Cell cycle
Cellular senescence

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