GFRAL Antibody

Datasheet

Citations (2)

Code	CSB-PA751020LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate(20µg), MCF-7 whole cell lysate(20µg), A549 whole cell lysate(20µg), 293 whole cell lysate(20µg), PC-3 whole cell lysate(20µg)Mouse Brain tissue lysate(20µg) All lanes: GFRAL antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 45 kDa Observed band size: 45 kDa Exposure time: 30s Immunofluorescence staining of Hela cell with CSB-PA751020LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GFRAL Polyclonal antibody

Uniprot No.

Q6UXV0

Target Names

GFRAL

Alternative Names

bA360D14.1 antibody; C6orf144 antibody; GDNF family receptor alpha-like antibody; Gfral antibody; GFRAL_HUMAN antibody; GRAL antibody; IVFI9356 antibody; UNQ9356 antibody; UNQ9356/PRO34128 antibody

Raised in

Rabbit

Species Reactivity

Human,mouse

Immunogen

Recombinant Human GDNF family receptor alpha-like protein (19-351AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:2000
IF	1:20-1:100

Protocols

Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

GFRAL polyclonal antibody CSB-PA751020LA01HU was produced in the rabbits immunized by using the Recombinant Human GDNF family receptor alpha-like protein (19-351AA). The target protein GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Upon interaction with its ligand (GDF15), GFRAL will interact with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.
This Rabbit anti-Homo sapiens (Human) GFRAL Polyclonal antibody was tested in WB, IHC and ELISA applications. The non-conjugated IgG got purified by protein G and reached up to 95% in purity. It reacts with the FLG proteins of human or mouse-origin and may be used to detect the endogenous levels of FLG protein.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Growth differentiation factor-15 stimulates the synthesis of corticotropin-releasing factor in hypothalamic 4B cells M Tasso,Peptides,2023
Growth differentiation factor-15 modulates adrenocorticotropic hormone synthesis in murine AtT-20 corticotroph cells K Kageyama,Peptides,2023

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Target Background

Function

Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses. Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKT- signaling pathways.

Subcellular Location

Cell membrane; Single-pass membrane protein; Extracellular side.

Protein Families

GDNFR family

Tissue Specificity

Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius (at protein level). Detected at low levels in testis and adipose tissue.

Database Links

HGNC: 32789

OMIM: 617837

KEGG: hsa:389400

STRING: 9606.ENSP00000343636

UniGene: Hs.526967

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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