KLRK1 Antibody

Code CSB-PA012474LA01HU
Size US$166
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  • Immunohistochemistry of paraffin-embedded human spleen tissue using CSB-PA012474LA01HU at dilution of 1:100

  • Immunofluorescent analysis of A549 cells using CSB-PA012474LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • Western Blot
    Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate
    All lanes: KLRK1 antibody at 3.7µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 26 kDa
    Observed band size: 34 kDa

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) KLRK1 Polyclonal antibody
Uniprot No.
Target Names
KLRK1
Alternative Names
KLRK1; D12S2489E; NKG2D; NKG2-D type II integral membrane protein; Killer cell lectin-like receptor subfamily K member 1; NK cell receptor D; NKG2-D-activating NK receptor; CD antigen CD314
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human NKG2-D type II integral membrane protein (73-216AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The KLRK1 Antibody (Product code: CSB-PA012474LA01HU) is Non-conjugated. For KLRK1 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA012474LB01HU KLRK1 Antibody, HRP conjugated ELISA
FITC CSB-PA012474LC01HU KLRK1 Antibody, FITC conjugated
Biotin CSB-PA012474LD01HU KLRK1 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The KLRK1 polyclonal antibody is a type of antibody that recognizes and binds to the human KLRK1 protein. The KLRK1 protein, also known as NKG2D, mainly functions to recognize and binds to stress-induced ligands expressed on the surface of infected or transformed cells, which allows the immune system to detect and eliminate these cells. KLRK1-mediated cytotoxicity is important for the immune response against viral infections and cancer, as well as for the elimination of cells undergoing cellular stress, such as cells undergoing DNA damage or apoptosis.

KLRK1 antibody is derived from multiple clones of B cells within the rabbit immunized with the recombinant human NKG2D protein (73-216aa), which produces a mixture of antibodies that can recognize different epitopes of the KLRK1 protein. The KLRK1 antibody underwent protein G affinity chromatography, getting a purity exceeding 95%. It has been quality validated in ELISA, WB, IHC, and IF applications.

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Target Background

Function
Functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, RAET1L/ULBP6, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4.
Subcellular Location
Cell membrane; Single-pass type II membrane protein. Note=Colocalized with HCST on the cell surface.
Tissue Specificity
Expressed in natural killer (NK) cells, CD8(+) alpha-beta and gamma-delta T-cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Expressed in interferon-producing killer dendritic cells (IKDCs).
Database Links

HGNC: 18788

OMIM: 611817

KEGG: hsa:100528032

STRING: 9606.ENSP00000240618

UniGene: Hs.387787

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