Natural killer cell (NK) has anti-tumor activity. Many kinds of receptors can be expressed on the surface of NK cells, which can be divided into active receptors and inhibitory receptors according to their function. NKG2D is a kind of activated receptors.
NKG2D was discovered by Houchins et al when screening the dominant expressed genes in human NK cells. NKG2D is also known as KLRK1, CD314, KLR and killer cell lectin-like receptor K1. It is a type Ⅱ transmembrane protein and a key member of the C-type lectin receptor family. It is an important activated receptor on the surface of immunoreactive cells such as NK cells, CD8 + γ δ T cells and γ δ T cells.
NKG2D receptors can specifically bind to NKG2D ligands on the surface of tumor cells and be activated, so as to achieve the purpose of killing tumor and play an important role in controlling tumor growth.
NKG2D was expressed in all NK cells, and was expressed in CD8 α β T cells, γ δ T cells, NKT, innate lymphoid cells (ILC) and CD4+T cells under some pathological conditions.
NKG2D can bind to many different ligands, which belong to major histocompatibility complex class I (MHC-I) related proteins.
The first NKG2D ligand of mice belongs to the RAE-1 protein family. The other two NKG2D ligands in mice are MULT-1 and minor histocompatibility antigen H60.
In humans, NKG2D ligands include MICA/B and ULBPs. Human NKG2D ligand RAET-1 protein is homologous to mouse RAE-1 protein. Another family of human NKG2D ligands are MICA and MICB.
These ligands are almost not expressed in healthy cells and tissues, but are highly expressed on the surface of target cells in the state of infection or tumor.
The interaction between NKG2D and its ligands makes immune cells proliferate, secrete cytokines such as IFN- γ, GM-CSF, MIP-1 α and IL-2, and even lead to the dissolution of target cells.
In NK cells, NKG2D acts as an activation receptor, activating NK's innate immune response and leading to cytotoxic activities. NK cells were induced to express NKG2D, which could regulate the differentiation and survival of NK cells and play an important role in the anti-tumor immune response. Activation of NKG2D can induce the secretion of cytokines and chemokines and promote the proliferation of effector cells. It is also involved in bone marrow transplantation rejection mediated by NK cells.
In CD8+T cells, NKG2D, as the costimulatory receptor of T cell receptor (TCR), sends costimulatory signals to enhance the activation of T cells. NKG2D partially regulates immune inflammation and enhances antiviral effect by activating NK cells during HBV infection.
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Recombinant Human NKG2-D type II integral membrane protein(KLRK1),partial (Active) (CSB-MP012474HU1)
Validated Data
Greater than 93% as determined by SDS-PAGE.
Immobilized KLRK1 at 10 μg/ml can bind human ULBP1 (CSB-MP887177HU), the EC50 of human KLRK1 protein is 222.4-276.0 ng/ml.
Human KLRK1 protein captured on COOH chip can bind Human ULBP1 protein with an affinity constant of 2.27 nM as detected by LSPR Assay.
Immobilized KLRK1 at 10 μg/ml can bind human Biotinylated ULBP1 (CSB-MP887177HUj1-B), the EC50 is 4.254-7.295 ng/ml.
The following KLRK1 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
KLRK1 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA008277 | KLRK1 Antibody | Human | WB, ELISA |
CSB-PA012474LA01HU | KLRK1 Antibody | Human | ELISA, WB, IHC, IF |
CSB-PA012474LB01HU | KLRK1 Antibody, HRP conjugated | Human | ELISA |
CSB-PA012474LC01HU | KLRK1 Antibody, FITC conjugated | Human | |
CSB-PA012474LD01HU | KLRK1 Antibody, Biotin conjugated | Human | ELISA |
CSB-PA888677 | KLRK1 Antibody | Human | IHC,ELISA |
KLRK1 Proteins for Pongo pygmaeus (Bornean orangutan)
Code | Product Name | Source |
---|---|---|
CSB-YP836611EXP CSB-EP836611EXP CSB-BP836611EXP CSB-MP836611EXP CSB-EP836611EXP-B |
Recombinant Pongo pygmaeus NKG2-D type II integral membrane protein (KLRK1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
KLRK1 Proteins for Macaca mulatta (Rhesus macaque)
Code | Product Name | Source |
---|---|---|
CSB-YP878778MOW CSB-EP878778MOW CSB-BP878778MOW CSB-MP878778MOW CSB-EP878778MOW-B |
Recombinant Macaca mulatta NKG2-D type II integral membrane protein (KLRK1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
KLRK1 Proteins for Sus scrofa (Pig)
Code | Product Name | Source |
---|---|---|
CSB-YP887907PI CSB-BP887907PI CSB-EP887907PI-B |
Recombinant Pig NKG2-D type II integral membrane protein (KLRK1), partial | Yeast Baculovirus In Vivo Biotinylation in E.coli |
KLRK1 Proteins for Pan troglodytes (Chimpanzee)
Code | Product Name | Source |
---|---|---|
CSB-YP889008EQV CSB-EP889008EQV CSB-BP889008EQV CSB-MP889008EQV CSB-EP889008EQV-B |
Recombinant Pan troglodytes NKG2-D type II integral membrane protein (KLRK1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
KLRK1 Proteins for Rattus norvegicus (Rat)
Code | Product Name | Source |
---|---|---|
CSB-CF012474RA | Recombinant Rat NKG2-D type II integral membrane protein (Klrk1) | in vitro E.coli expression system |
CSB-YP012474RA1 CSB-EP012474RA1 CSB-BP012474RA1 CSB-MP012474RA1 CSB-EP012474RA1-B |
Recombinant Rat NKG2-D type II integral membrane protein (Klrk1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
KLRK1 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-CF012474HU | Recombinant Human NKG2-D type II integral membrane protein (KLRK1) | in vitro E.coli expression system |
CSB-MP012474HU1 | Recombinant Human NKG2-D type II integral membrane protein (KLRK1), partial (Active) | Mammalian cell |
CSB-YP012474HU1 CSB-EP012474HU1 CSB-BP012474HU1 CSB-EP012474HU1-B |
Recombinant Human NKG2-D type II integral membrane protein (KLRK1), partial | Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli |
KLRK1 Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
Code | Product Name | Source |
---|---|---|
CSB-CF012474MOV | Recombinant Macaca fascicularis NKG2-D type II integral membrane protein (KLRK1) | in vitro E.coli expression system |
CSB-YP012474MOV1 CSB-EP012474MOV1 CSB-BP012474MOV1 CSB-MP012474MOV1 CSB-EP012474MOV1-B |
Recombinant Macaca fascicularis NKG2-D type II integral membrane protein (KLRK1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
KLRK1 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-CF012474MO | Recombinant Mouse NKG2-D type II integral membrane protein (Klrk1) | in vitro E.coli expression system |
CSB-YP012474MO1 CSB-EP012474MO1 CSB-BP012474MO1 CSB-MP012474MO1 CSB-EP012474MO1-B |
Recombinant Mouse NKG2-D type II integral membrane protein (Klrk1), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |