PECAM1 Antibody

Datasheet
Code CSB-PA116222
Size US$167
Image
  • Western Blot analysis of Hela cells using primary antibody diluted at 1:1000(4°C overnight). Secondary antibody:Goat Anti-rabbit IgG IRDye 800( diluted at 1:5000, 25°C, 1 hour)

Product Details

Uniprot No. P16284
Target Names PECAM1
Alternative Names Platelet endothelial cell adhesion molecule (PECAM-1) (EndoCAM) (GPIIA\') (PECA1) (CD antigen CD31)
Raised in Rabbit
Species Reactivity Human
Immunogen Synthesized peptide derived from Human CD31. at AA range: Internal
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Purification Method The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Form Liquid
Tested Applications WB,ELISA
Recommended Dilution
Application Recommended Dilution
WB 1:500-2000
ELISA 1:10000-20000
Protocols Western Blotting(WB) Protocol
ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions
Subcellular Location Cell membrane, Single-pass type I membrane protein, Note=Cell surface expression on neutrophils is down-regulated upon fMLP or CXCL8/IL8-mediated stimulation, SUBCELLULAR LOCATION: Isoform Long: Cell membrane, Single-pass type I membrane protein, Membrane raft, Cell junction, Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells, SUBCELLULAR LOCATION: Isoform Delta15: Cell junction
Tissue Specificity Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells (PubMed:18388311, PubMed:21464369). Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level) (PubMed:19342684, PubMed:17580
Database Links

HGNC: 8823

OMIM: 173445

KEGG: hsa:5175

UniGene: Hs.376675

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