PRKACA Antibody, FITC conjugated

Datasheet
Code CSB-PA018688LC01HU
Size US$299

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) PRKACA Polyclonal antibody
Uniprot No. P17612
Target Names PRKACA
Alternative Names cAMP-dependent protein kinase catalytic subunit alpha (PKA C-alpha) (EC 2.7.11.11), PRKACA, PKACA
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human cAMP-dependent protein kinase catalytic subunit alpha protein (233-351AA)
Immunogen Species Homo sapiens (Human)
Conjugate FITC
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Target Data

Function Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, PSMC5/RPT6, PJA2, RYR2, RORA and VASP. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts. Involved in the regulation of platelets in response to thrombin and collagen; maintains circulating platelets in a resting state by phosphorylating proteins in numerous platelet inhibitory pathways when in complex with NF-kappa-B (NFKB1 and NFKB2) and I-kappa-B-alpha (NFKBIA), but thrombin and collagen disrupt these complexes and free active PRKACA stimulates platelets and leads to platelet aggregation by phosphorylating VASP. Prevents the antiproliferative and anti-invasive effects of alpha-difluoromethylornithine in breast cancer cells when activated. RYR2 channel activity is potentiated by phosphorylation in presence of luminal Ca(2+), leading to reduced amplitude and increased frequency of store overload-induced Ca(2+) release (SOICR) characterized by an increased rate of Ca(2+) release and propagation velocity of spontaneous Ca(2+) waves, despite reduced wave amplitude and resting cytosolic Ca(2+). PSMC5/RPT6 activation by phosphorylation stimulates proteasome. Negatively regulates tight junctions (TJs) in ovarian cancer cells via CLDN3 phosphorylation. NFKB1 phosphorylation promotes NF-kappa-B p50-p50 DNA binding. Involved in embryonic development by down-regulating the Hedgehog (Hh) signaling pathway that determines embryo pattern formation and morphogenesis. Prevents meiosis resumption in prophase-arrested oocytes via CDC25B inactivation by phosphorylation. May also regulate rapid eye movement (REM) sleep in the pedunculopontine tegmental (PPT). Phosphorylates APOBEC3G and AICDA. Isoform 2 phosphorylates and activates ABL1 in sperm flagellum to promote spermatozoa capacitation. Phosphorylates HSF1; this phosphorylation promotes HSF1 nuclear localization and transcriptional activity upon heat shock
Involvement in disease Primary pigmented nodular adrenocortical disease 4 (PPNAD4)
Subcellular Location Cytoplasm, Cell membrane, Nucleus, Mitochondrion, Membrane, Lipid-anchor, Note=Translocates into the nucleus (monomeric catalytic subunit), The inactive holoenzyme is found in the cytoplasm, Distributed throughout the cytoplasm in meiotically incompetent oocytes, Associated to mitochondrion as meiotic competence is acquired, Aggregates around the germinal vesicles (GV) at the immature GV stage oocytes (By similarity), Colocalizes with HSF1 in nuclear stress bodies (nSBs) upon heat shock (PubMed:21085490), SUBCELLULAR LOCATION: Isoform 2: Cell projection, cilium, flagellum, Cytoplasmic vesicle, secretory vesicle, acrosome
Protein Families Protein kinase superfamily, AGC Ser/Thr protein kinase family, cAMP subfamily
Tissue Specificity Isoform 1 is ubiquitous. Isoform 2 is sperm-specific and is enriched in pachytene spermatocytes but is not detected in round spermatids.
Database Links

HGNC: 9380

OMIM: 601639

KEGG: hsa:5566

STRING: 9606.ENSP00000309591

UniGene: Hs.631630

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