PRKACB Antibody

Datasheet

Code	CSB-PA018689LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: A549 whole cell lysate, MCF-7 whole cell lysate, PC-3 whole cell lysate, Rat brain tissue, Mouse brain tissue All lanes: PRKACB antibody at 3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 41, 47, 40, 42, 30, 37 kDa Observed band size: 41, 47 kDa Immunohistochemistry of paraffin-embedded human brain tissue using CSB-PA018689LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human kidney tissue using CSB-PA018689LA01HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA018689LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) PRKACB Polyclonal antibody

Uniprot No.

P22694

Target Names

PRKACB

Alternative Names

cAMP-dependent protein kinase catalytic beta subunit isoform 4ab antibody; cAMP-dependent protein kinase catalytic subunit beta antibody; KAPCB_HUMAN antibody; PKA C beta antibody; PKA C-beta antibody; PKACB antibody; Prkacb antibody; protein kinase A catalytic subunit beta antibody; Protein kinase cAMP dependent catalytic beta antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human cAMP-dependent protein kinase catalytic subunit beta protein (2-53AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The PRKACB Antibody (Product code: CSB-PA018689LA01HU) is Non-conjugated. For PRKACB Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA018689LB01HU	PRKACB Antibody, HRP conjugated	ELISA
FITC	CSB-PA018689LC01HU	PRKACB Antibody, FITC conjugated
Biotin	CSB-PA018689LD01HU	PRKACB Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. Phosphorylates GPKOW which regulates its ability to bind RNA.

Gene References into Functions

Cbeta2 subunit of protein kinase A mRNA was up-regulated in prostate cancer and low expression of Cbeta2 mRNA in prostate cancer biopsies correlated with poor survival. PMID: 27838142
analysis of the mechanism underlying synergistic up-regulation of PDE4B2 via a cross-talk between PKA-Cbeta and p65 PMID: 25831493
The gene polymorphism loci rs12132032 in PRKACB maybe a potential risk factor for anencephaly in Chinese population from Shanxi, while gender susceptibility may influence the correlation. PMID: 24294386
The previously unknown small molecule inhibitor-dependent interaction of Cbeta1 with the cell cycle and apoptosis regulatory protein-1 was verified. PMID: 20564261
activated by podophyllotoxin PMID: 20033853
PGE(2)-induced CYP1B1 expression is mediated by ligand-independent activation of the ERalpha pathway as a result of the activation of ERK, Akt, and PKA in breast cancer cells. PMID: 20093341
Data show that PI3K activation and PIP3 production lead to recruitment of the PKB/beta-arrestin/PDE4 complex to the membrane via the PKB PH domain, resulting in degradation of the TCR-induced cAMP pool and allowing full T-cell activation to proceed. PMID: 20086095
c-MYC induces the activity of protein kinase A by inducing the transcription of the gene encoding the PKA catalytic subunit beta in multiple tissues, independent of cell proliferation by direct binding of c-MYC to promoter sequences. PMID: 12420224
Data describe the identification of a variant of the beta catalytic subunit of cyclic AMP-dependent protein kinase (PKACbeta) as a p75 neurotrophin receptor(NTR)-interacting protein, which phosphorylates p75(NTR) at Ser304. PMID: 12682012
there are abnormalities in [3H]cAMP binding and catalytic activity kinase A in brain of depressed suicide victims, which could be due to reduced expression of RIIbeta and Cbeta. PMID: 14744463
there is a PKA-Cbeta-mediated inhibitory mechanism of p73 function PMID: 15723830
Murine lymphoid tissues express a protein that is a homologue of human protein kinase c subunit beta2 PMID: 16889664
In conclusion, it seems that the Cbeta isoforms of PKA play different roles in proliferation and differentiation and could therefore be potential markers for prostate cancer progression. PMID: 16949795
Data provide the first evidence that Protein kinase C -beta play pivotal role in the regulation of AA production and cellular proliferation of human monocytoid MonoMac-6 cells. PMID: 17549442
Recruitment of coactivator glucocorticoid receptor interacting protein 1 to an estrogen receptor transcription complex is regulated by the 3',5'-cyclic adenosine 5'-monophosphate-dependent protein kinase. PMID: 18499756

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Subcellular Location

Cytoplasm. Cell membrane. Membrane; Lipid-anchor. Nucleus.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, cAMP subfamily

Tissue Specificity

Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain.

Database Links

HGNC: 9381

OMIM: 176892

KEGG: hsa:5567

STRING: 9606.ENSP00000359719

UniGene: Hs.487325

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Calcium signaling pathway cAMP signaling pathway Chemokine signaling pathway Estrogen signaling pathway Hedgehog signaling pathway MAPK signaling pathway Melanogenesis Wnt signaling pathway Gap junction Vascular smooth muscle contraction Autophagy Ras signaling pathway Th17 cell differentiation Apelin signaling pathway Glucagon signaling pathway Insulin signaling pathway Glutamatergic synapse G protein coupled receptor signaling pathway

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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