Phospho-NR3C1 (Ser226) Antibody

Datasheet
Code CSB-PA298129
Product Type Polyclonal Antibody
Size US$360
Uniprot No. P04150
Image
  • Western blot analysis of extracts from Jurkat cells treated with EGF using Phospho-GR (Ser226) antibody.The lane on the right is treated with the antigen-specific peptide.
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunogen Peptide sequence around phosphorylation site of serine 226 (L-L-S(p)-P-L) derived from Human GR.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous level of GR only when phosphorylated at serine 226.
Tested Applications ELISA,WB;WB:1:500-1:1000
Relevance

Receptor for glucocorticoids (GC). Has a dual mode of action: as a transcription factor that binds to glucocorticoid response elements (GRE), both for nuclear and mitochondrial DNA, and as a modulator of other transcription factors. Affects inflammatory responses, cellular proliferation and differentiation in target tissues. Could act as a coactivator for STAT5-dependent transcription upon growth hormone (GH) stimulation and could reveal an essential role of hepatic GR in the control of body growth. Involved in chromatin remodeling. Plays a significant role in transactivation.

Wehmeyer L, Du Toit A, Lang DM, Hapgood JP (2014) J Biol Chem 289, 10235-51
Bouazza B, et al. (2014) Am J Respir Cell Mol Biol 50, 301-15
Lee MS, et al. (2013) Behav Brain Res 236, 56-61

Form Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Clonality Polyclonal
Alias GCCR; GCR; GRL; NR3C1;
Immunogen Species Homo sapiens (Human)
Target Names NR3C1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Receptor for glucocorticoids (GC)
Involvement in disease Glucocorticoid resistance, generalized (GCCR)
Subcellular Location Isoform Alpha: Cytoplasm, Nucleus, Mitochondrion, Cytoplasm, cytoskeleton, spindle, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Note=After ligand activation, translocates from the cytoplasm to the nucleus, SUBCELLULAR LOCATION: Isoform Beta: Nucleus, Cytoplasm, Note=Expressed predominantly in the nucleus with some expression also detected in the cytoplasm, SUBCELLULAR LOCATION: Isoform Alpha-B: Nucleus, Cytoplasm
Protein Families Nuclear hormone receptor family, NR3 subfamily
Tissue Specificity Widely expressed including bone, stomach, lung, liver, colon, breast, ovary, pancreas and kidney (PubMed:25847991). In the heart, detected in left and right atria, left and right ventricles, aorta, apex, intraventricular septum, and atrioventricular node
Database Links

HGNC: 7978

OMIM: 138040

KEGG: hsa:2908

STRING: 9606.ENSP00000231509

UniGene: Hs.122926

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