Phospho-PRKAA1/PRKAA2 (Ser487) Antibody

Datasheet
Code CSB-PA261465
Product Type Polyclonal Antibody
Size US$360
Uniprot No. Q13131
Image
  • Western blot analysis of extracts from HUVEC cells untreated or treated with UV using AMPKa1(Phospho-Ser487)Antibody.
  • Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using AMPKa1(Phospho-Ser487)Antibody(left) or the same antibody preincubated with blocking peptide(right).
  • Immunofluorescence staining of methanol-fixed Hela cells using AMPKa1(Phospho-Ser487)Antibody.
  • Western blot analysis of extracts from C6 cells, treated with EGF or calf intestinal phosphatase (CIP), using AMPKα1 (Phospho-Ser487) Antibody.
Immunogen Peptide sequence around phosphorylation site of serine 487 (S-G- SP-V-S) derived from Human AMPKa1.
Raised in Rabbit
Species Reactivity Human,Mouse,Rat
Specificity The antibody detects endogenous level of AMPKa1 only when phosphorylated at serine 487.
Tested Applications ELISA,WB,IHC,IF;WB:1:500-1:1000,IHC:1:50-1:100,IF:1:100-1:200
Relevance

Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.

Kim JE, et al. (2005) J Proteome Res. 4(4): 1339-1346.
Woods A, et al. (2003) J Biol Chem. 278(31): 28434-28442.

Form Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Clonality Polyclonal
Alias AAPK1; AMPK alpha-1 chain; AMPK-alpha1; HMG-CoA redustase kinase; PRKAA1
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Target Names PRKAA1/PRKAA2
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes
Subcellular Location Cytoplasm, Nucleus
Protein Families Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
Database Links

HGNC: 9376

OMIM: 602739

KEGG: hsa:5562

STRING: 9606.ENSP00000346148

UniGene: Hs.43322

Pathway mTOR signaling pathway
PI3K-Akt signaling pathway
Autophagy
Adipocytokine signaling pathway
AMPK signaling
Apelin signaling pathway
FoxO signaling pathway
Glucagon signaling pathway
Insulin signaling pathway

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