SARS Antibody

Datasheet

Code	CSB-PA04145A0Rb
Size	US$166
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Image	Western blot All lanes: SARS antibody at 2µg/ml Lane 1: EC109 whole cell lysate Lane 2: 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 59 kDa Observed band size: 59 kDa Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA04145A0Rb at dilution of 1:100 Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA04145A0Rb at dilution of 1:100
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SARS Polyclonal antibody

Uniprot No.

P49591

Target Names

SARS

Alternative Names

C78314 antibody; cytoplasmic antibody; EC 6.1.1.11 antibody; FLJ36399 antibody; sarS antibody; Sars1 antibody; Serine tRNA ligase 1, cytoplasmic antibody; Serine tRNA ligase antibody; Serine--tRNA ligase antibody; serine--tRNA ligase, cytoplasmic antibody; SerRS antibody; SERS antibody; Seryl tRNA Ser/Sec synthetase antibody; Seryl tRNA synthetase antibody; Seryl tRNA synthetase cytoplasmic antibody; Seryl-tRNA Ser/Sec synthetase antibody; Seryl-tRNA synthetase antibody; seryl-tRNA synthetase, cytoplasmic antibody; Seryl-tRNA(Ser/Sec) synthetase antibody; Strs antibody; SYSC_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Serine--tRNA ligase, cytoplasmic protein (2-233AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SARS Antibody (Product code: CSB-PA04145A0Rb) is Non-conjugated. For SARS Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA04145B0Rb	SARS Antibody, HRP conjugated	ELISA
FITC	CSB-PA04145C0Rb	SARS Antibody, FITC conjugated
Biotin	CSB-PA04145D0Rb	SARS Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Immunization of rabbits with the peptide corresponding to amino acids 2-233 of the human serine-tRNA ligase and subsequent protein G -mediated purification from anti-serum lead to the generation of the SARS antibody. This SARS antibody is a polyclonal antibody and exists as an unconjugated IgG. Its purity is 95%+. It is only reactive with human Serine tRNA ligase and does not cross-react with other species. Validations of this antibody in ELISA, WB, and IHC have been tested. The target protein serine-tRNA ligase is an enzyme that catalyzes the chemical reaction. It is involved in glycine, serine, and threonine metabolism and aminoacyl-tRNA biosynthesis.

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Target Background

Function

Catalyzes the attachment of serine to tRNA(Ser) in a two-step reaction: serine is first activated by ATP to form Ser-AMP and then transferred to the acceptor end of tRNA(Ser). Is probably also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec). In the nucleus, binds to the VEGFA core promoter and prevents MYC binding and transcriptional activation by MYC. Recruits SIRT2 to the VEGFA promoter, promoting deacetylation of histone H4 at 'Lys-16' (H4K16). Thereby, inhibits the production of VEGFA and sprouting angiogenesis mediated by VEGFA.

Gene References into Functions

Here, we report on mutations in two aminoacyl-tRNA synthetases that are associated with Intellectual disability (ID) in two unrelated Iranian families. In the first family, we identified a homozygous missense mutation (c.514G>A, p.Asp172Asn) in the cytoplasmic seryl-tRNA synthetase (SARS) gene PMID: 28236339
Analyzed the ability of cytosolic SerRS to bind and act on tRNA(Ser), tRNA(Sec), and 10 mutant and chimeric constructs in which elements of tRNA(Ser) were transposed onto tRNA(Sec). We show that SerRS only subtly prefers tRNA(Ser) to tRNA(Sec), and that discrimination occurs at the level of the serylation reaction. PMID: 28808125
Yin Yang 1 (YY1) interacts with Seryl-tRNA synthetase (SerRS) to form a SerRS/YY1 complex which functions as a negative effector to regulate vegfa transcription through binding a distal segment of the vegfa promoter, while NFKB1 serves as a positive effector through outcompeting SerRS/YY1 for binding at the same distal segment. PMID: 27913726
tRNA recognition mode of SerRS. PMID: 26433229
Several data support that p.R402H mutation in SARS2 is a new cause of HUPRA syndrome. PMID: 24034276
A long-range conformational and functional communication specific to higher eukaryotes is found in human SerRS. PMID: 24095058
study identified a nuclear localization signal sequence embedded in UNE-S domain and showed that the essential role of SerRS in vascular development is dependent on UNE-S and its role to mobilize SerRS from the cytoplasm to the nucleus PMID: 22353712
Mutations in the mitochondrial seryl-tRNA synthetase cause hyperuricemia, pulmonary hypertension, renal failure in infancy and alkalosis, HUPRA syndrome. PMID: 21255763
Recombinant human cytosolic seryl-tRNA synthetase protein was purified to homogeneity and crystallized. Diffraction data were collected to 3.13 A resolution. PMID: 21045311
SARS regulates endothelial sprouting. These analyses of zebrafish and human endothelial cells reveal a new noncanonical function of Sars in endothelial development. PMID: 19423847

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Class-II aminoacyl-tRNA synthetase family, Type-1 seryl-tRNA synthetase subfamily

Tissue Specificity

Brain.

Database Links

HGNC: 10537

OMIM: 607529

KEGG: hsa:6301

STRING: 9606.ENSP00000234677

UniGene: Hs.531176

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