SOS2 Antibody

Code CSB-PA22327A0Rb
Size US$166
Order now
Image
  • Western Blot
    Positive WB detected in: A549 whole cell lysate, SH-SY5Y whole cell lysate, HL60 whole cell lysate, K562 whole cell lysate, PC-3 whole cell lysate, Hela whole cell lysate
    All lanes: SOS2 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 153, 150 kDa
    Observed band size: 153 kDa

  • IHC image of CSB-PA22327A0Rb diluted at 1:100 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA22327A0Rb diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of A549 cells with CSB-PA22327A0Rb at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) SOS2 Polyclonal antibody
Uniprot No.
Target Names
SOS2
Alternative Names
FLJ25596 antibody; Guanine nucleotide exchange factor antibody; Guanine nucleotide releasing factor antibody; Son of sevenless (Drosophilia) homolog 2 antibody; Son of sevenless homolog 2 (Drosophila) antibody; Son of sevenless homolog 2 antibody; SOS 2 antibody; SOS-2 antibody; Sos2 antibody; SOS2_HUMAN antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Son of sevenless homolog 2 protein (187-404AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The SOS2 Antibody (Product code: CSB-PA22327A0Rb) is Non-conjugated. For SOS2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA22327B0Rb SOS2 Antibody, HRP conjugated ELISA
FITC CSB-PA22327C0Rb SOS2 Antibody, FITC conjugated
Biotin CSB-PA22327D0Rb SOS2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Promotes the exchange of Ras-bound GDP by GTP.
Gene References into Functions
  1. Activating Mutations Affecting the Dbl Homology Domain of SOS2 Cause Noonan Syndrome PMID: 26173643
  2. We identified two novel genes, SOS2 and LZTR1, associated with Noonan syndrome, thereby expanding the molecular spectrum of RASopathies. PMID: 25795793
  3. multicenter pharmacogenetic study in children (ages 6-11): Data suggest that SNP in SOS2 (rs13379306) in children with growth hormone deficiency is associated with drug resistance to hormone replacement therapy with recombinant human growth hormone. PMID: 23761422
  4. intracellular Francisella tularensis novicida triggers temporal and early activation of Ras through the SOS2/GrB2/PKCalpha/PKCbetaI quaternary complex; Ras signalling by intracellular F. tularensis is essential for intracellular proliferation in the cytosol PMID: 20618341
  5. Significant association of single nucleotide polymorphism within three genes--PPARgamma, SOS2, and PCK1--with Alzheimer's disease, was confirmed. PMID: 17440948

Show More

Hide All

Involvement in disease
Noonan syndrome 9 (NS9)
Database Links

HGNC: 11188

OMIM: 601247

KEGG: hsa:6655

STRING: 9606.ENSP00000216373

UniGene: Hs.291533

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*