TIPARP Antibody

Datasheet

Code	CSB-PA801800LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: U87 whole cell lysate, SH-SY5Y whole cell lysate All lanes: TIPARP antibody at 4.6µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 77 kDa Observed band size: 77 kDa IHC image of CSB-PA801800LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA801800LA01HU diluted at 1:300 and staining in paraffin-embedded human glioma performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TIPARP Polyclonal antibody

Uniprot No.

Q7Z3E1

Target Names

TIPARP

Alternative Names

ADP-ribosyltransferase diphtheria toxin-like 14 antibody; ARTD14 antibody; AW558171 antibody; DDF1 antibody; DKFZp434J214 antibody; DKFZp686N0351 antibody; DKFZp686P1838 antibody; FLJ40466 antibody; PARP 1 antibody; PARP 7 antibody; PARP-7 antibody; PARPT_HUMAN antibody; pART14 antibody; Poly [ADP ribose] polymerase 7 antibody; Poly [ADP-ribose] polymerase 7 antibody; TCDD inducible poly(ADP ribose) polymerase antibody; TCDD-inducible poly [ADP-ribose] polymerase antibody; TIPARP antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human TCDD-inducible poly [ADP-ribose] polymerase protein (74-178AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TIPARP Antibody (Product code: CSB-PA801800LA01HU) is Non-conjugated. For TIPARP Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA801800LB01HU	TIPARP Antibody, HRP conjugated	ELISA
FITC	CSB-PA801800LC01HU	TIPARP Antibody, FITC conjugated
Biotin	CSB-PA801800LD01HU	TIPARP Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Rabbit polyclonal antibody against TIPARP is developed in a rabbit. The peptide sequence for the immunogen was taken from within the amino acids 74-178 of the recombinant human TIPARP protein. This TIPARP antibody is purified by protein G and got a purity of up to 95%. Three applications including ELISA, WB, and IHC have been used in assays to test for the specificity of this TIPARP antibody.

TIPARP primarily acts as a poly(ADP-ribose) polymerase (PARP) enzyme that is involved in the regulation of DNA repair, chromatin structure, and gene expression. It has also been implicated in the regulation of gene expression, particularly in response to cellular stress and inflammation. TIPARP dysregulation is related to various pathological conditions, including cancer, neurodegenerative diseases, and autoimmune disorders.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate, aspartate and cysteine residues on target proteins. Acts as a negative regulator of AHR by mediating mono-ADP-ribosylation of AHR, leading to inhibit transcription activator activity of AHR.

Gene References into Functions

TIPARP is a viral RNA-sensing pattern recognition receptors that mediates antiviral responses triggered by BAX- and BAK1-dependent mitochondrial damage PMID: 28213497
Moreover, time course and promoter activity assays suggest that TIPARP and TIPARP-AS1 work in concert to regulate AHR signaling. Collectively, these data show an added level of complexity in the AHR signaling cascade which involves lncRNAs, whose functions remain poorly understood. PMID: 29274782
Knockdown of TiPARP, but not AHRR, increased 2,3,7,8-tetrachlorodibenzo-p-dioxin - induced CYP1A1 mRNA and AHR protein levels. PMID: 24806346
Single nucleotide polymorphism in TIPARP is associated with ovarian cancer. PMID: 20852632
TIPARP (DKFZp434J214) gene is amplified in HNSCC. TIPARP, FLJ22693 and ZAP proteins with TPH, WW and PARP-like domains constitute the TIPARP family. PMID: 12851707

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Subcellular Location

Nucleus.

Database Links

HGNC: 23696

OMIM: 612480

KEGG: hsa:25976

STRING: 9606.ENSP00000295924

UniGene: Hs.744050

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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