Tgfb1 Antibody

Code CSB-PA023446LA01MO
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
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  • Western Blot
    Positive WB detected in: MCF-7 whole cell lysate
    All lanes: Tgfb1 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Immunoprecipitating Tgfb1 in MCF-7 whole cell lysate
    Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA023446LA01MO in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA023446LA01MO (8μg) + MCF-7 whole cell lysate (500μg)
    Lane 3: MCF-7 whole cell lysate (10μg)

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Product Details

Full Product Name Rabbit anti-Mus musculus (Mouse) Tgfb1 Polyclonal antibody
Uniprot No. P04202
Target Names Tgfb1
Alternative Names Tgfb1 antibody; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide antibody; LAP); Transforming growth factor beta-1 antibody; TGF-beta-1)] antibody
Raised in Rabbit
Species Reactivity Mouse, Human
Immunogen Recombinant Mouse Transforming growth factor beta-1 protein (30-278AA)
Immunogen Species Mus musculus (Mouse)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IP 1:200-1:2000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Megakaryocytes participate in the occurrence of bleomycin-induced pulmonary fibrosis. Yan Zhou, et al,Cell Death & Disease,2019

Applications: WB
Antibody dilution factor: 1: 500
Review: The level of TGF-β1 of M-07e inducing with different concentration TPO evaluated by western blotting (n=3, **P <0.01 vs control group).

Target Data

Function Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts
Gene References into Functions
  1. this paper shows that epithelial-derived TGF-beta1 acts as a pro-viral factor in the lung during influenza A infection PMID: 29067998
  2. Study points toward elevated levels of active TGF-beta as inducers and promoters of ectopic bone formation, and suggest that TGF-beta might be a therapeutic target in heterotopic ossification. PMID: 29416028
  3. The comparison of transforming growth factor beta family (TGFbeta) expression showed significantly higher levels of Tgfbeta3 transcript between nude and Balb/c mice but no differences were detected for Tgfbeta1. Nude DFs were specifically sensitive to the presence of the pro-regenerative TGFbeta3 isoform, showing increased collagen I deposition and alpha smooth muscle actin expression. PMID: 29637306
  4. Results indicate that the miR-23a cluster regulates osteocyte differentiation by modulating the TGF-beta signalling pathway. PMID: 28397831
  5. Following Schistosoma exposure, TSP-1 levels in the lung increase, via recruitment of circulating monocytes, while TSP-1 inhibition or knockout bone marrow prevents TGF-beta activation and protects against pulmonary hypertension development. PMID: 28555642
  6. TGF-beta/Smad proteins signaling affects radiation response and prolongs survival by regulating DNA repair genes in malignant glioma. PMID: 30230914
  7. The results indicate that EGFR and its activation are critical for YAP-mediated suppression of TGF-beta1-induced apoptosis. This study provides a new understanding of the regulatory mechanism underlying the determination of cell fate in response to TGF-beta1-mediated simultaneous apoptosis and epithelial mesenchymal transformation. PMID: 28425446
  8. transforming growth factor beta (TGFbeta) signaling was upregulated in HSCs from bone marrow of mice with MLL-AF9-induced acute myeloid leukemia (AML) because of excessive production of TGFbeta1, especially from megakaryocytes, and overactivation of latent TGFbeta1 protein. PMID: 29307605
  9. TGF-beta release from platelets is necessary for podoplanin-mediated tumor invasion and metastasis in lung cancer. PMID: 28176852
  10. Data suggest partial or complete transforming growth factor beta 1 (TGFBI) knockdown as a potential therapy against TGFBI-linked corneal dystrophies. PMID: 29117645
  11. De novo formation of the biliary system by TGFbeta-mediated hepatocyte transdifferentiation PMID: 29720662
  12. CXCL9 may promote prostate cancer progression via inhibition of cytokines from T cells. PMID: 29901197
  13. EGCG attenuated airway inflammation in asthmatic mice, decreased the percentage of Th17 cells and increased the percentage of Treg cells. The antiinflammatory effect of EGCG is achieved via the TGFbeta1 signaling pathway. PMID: 29916550
  14. fibrosis induced by Ang II may be alleviated by AKAP12 expression through inactivation of the TGF-beta1 pathway. PMID: 29501491
  15. RUNX1 promoted TGF-beta-induced partial EMT by increasing transcription of the PI3K subunit p110delta, which mediated Akt activation. PMID: 29759484
  16. Transforming growth factor-beta1 induces cerebrovascular dysfunction and astrogliosis through angiotensin II type 1 receptor-mediated signaling pathways PMID: 29505736
  17. TET2 plays an important role in the pathogenesis of diabetic nephropathy by activating TGFbeta1 expression through demethylation of CpG islands in the TGFbeta1 regulatory region PMID: 29705354
  18. early myocardial CTGF mRNA expression (six hours) after Ang-II exposure is likely dependent on latent TGF-beta activation via the canonical Smad-dependent pathway in resident cardiac cells. PMID: 29575960
  19. These results indicate that the hepatocytic expression of TGF-beta and CTGF is mediated by Wnt signalling in Schistosoma japonicum infection. PMID: 28331224
  20. Infection with Mycobacterium bovis results in increase in interleukin-1alpha, TGF-beta1, and MMP1 in multinucleated macrophages. PMID: 29504104
  21. type I and II IFN as in vivo modifiers of LIC fate that may significantly affect the impact of putative leukemia-driving activities such as the ETV6-RUNX1-mediated growth advantage in the presence of TGF-beta and TLR4-upregulated mutagenic activity PMID: 27220664
  22. Studies indicate key developmental processes that require transforming growth factor beta (TGF-beta) signaling [Review]. PMID: 29190317
  23. Osthole,a natural coumarin, decreases the collagen/III ratio in transforming growth factor beta 1 (TGF-beta1)-transfected mouse cardiac fibroblasts via the TGF-beta/Smad signaling pathway. PMID: 29860992
  24. both ERK and Smad2 signal pathways are involved in the activation of macrophages induced by TGF-b1 and high-ambient glucose, while there is no crosstalk shown in the process. PMID: 29199516
  25. In obese mice, periodontitis caused the downregulation of MMP2, and upregulation of TIMP1 and TGF-beta1 at transcriptional and translational levels PMID: 29322806
  26. The protective effect of the EP2 receptor on TGF-beta1 induced podocyte injury via the PI3K / Akt signaling pathway. PMID: 29746568
  27. This study demonstrates that prevention of renal apoB accumulation is a mechanism by which TGF-beta inhibition is nephroprotective. PMID: 28912302
  28. data show that increased TGFbeta in the tumour microenvironment represents a primary mechanism of immune evasion that promotes T-cell exclusion and blocks acquisition of the TH1-effector phenotype; immunotherapies directed against TGFbeta signalling may therefore have broad applications in treating patients with advanced colorectal cancer PMID: 29443964
  29. IL 6 and TGF beta perform essential role in cerebral malaria pathogenesis by modulating the level of glial cell induced neuroinflammation. PMID: 28803696
  30. The increased susceptibility to IMQ-induced psoriasis of GILZ-Tg mice was significantly associated with skin-specific over-activation of TGF-beta1-mediated signaling via SMAD2/3. PMID: 27934944
  31. The data suggest that B cells can down-regulate the function of antigen-presenting cells, and in turn encephalitogenic Th1/Th17 responses, via TGF-beta1. PMID: 27708418
  32. p-SMAD2/3 and p-ERK1/2 might play a regulatory role in TGF-beta1 induced CTGF exp p-SMAD2/3 and p-ERK1/2 might play a regulatory role in TGF-beta1 induced CTGF expression during tooth development. PMID: 28825193
  33. inhibiting NCAM1 would be cardioprotective, counteract the pathological action of TGFbeta1 and reduce heart failure severity. PMID: 28870505
  34. TGF-beta signaling has a role in inhibiting tumor progression and invasion in an induced mouse bladder cancer model PMID: 27378170
  35. calpains inhibition plays crucial roles in vascular restenosis by preventing neointimal hyperplasia at the early stage via suppression of the MMP2/TGF-beta1 pathway. PMID: 27453531
  36. Suggest an essential role for platelet-derived TGFbeta1 for the vascular remodelling response to arterial injury, apparently independent from the role of platelets in thrombosis or haemostasis. PMID: 28726976
  37. In cultured B16 melanoma and Bend3 endothelial cells treated with Bend3 conditioned media, MITF, tyrosinase, and melanin expression decreased due to TGFB1 secreted by the endothelial cells. PMID: 27172887
  38. Lycat regulates TGF-beta mediated lung fibroblast differentiation in pulmonary fibrosis. PMID: 28751023
  39. TFGbeta1 and arachidonic acid synthase expression is increased during the involution period in mammary glands. PMID: 28381667
  40. Chronic exercise suppressed the TGF-beta1/IkB-alpha axis in the hypothalamus and improved the energy homeostasis in an animal model of obesity-associated with aging. PMID: 28854149
  41. miR-146 inhibits skeletal muscle fibrosis by suppressing TGF-beta/Smad4 signaling pathway. PMID: 28510617
  42. Study shows that expression of Gadd45 family members is adjusted in response to KCl/depolarization, BDNF/TRKB, and/or TGFB-signaling. Additionally, results indicate decreased Gadd45 expression in a mouse model of depression, and that Gadd45b expression and DNA demethylation activate Arc transcription. PMID: 28444170
  43. Bioinformatics analysis provides positive supportive correlation between somatic mutations for VD-related genes and the TGF-beta pathway PMID: 27456065
  44. Taken together, these results indicate that eIF6 may be involved in external mechanical force-mediated murine dermal fibroblast function at least partly through the TGF-beta1/TGFBR1/TGFBR2 pathway. PMID: 27824055
  45. important roles in virtually every stage of neural development [review] PMID: 28130363
  46. These data suggest that the interplay between cell-matrix adhesion and intercellular adhesion is an important determinant for some aspects of TGFbeta1-induced epithelial-mesenchymal transition via alphaSMA expression induction. PMID: 27194451
  47. Excessive activation of TGFbeta by spinal instability causes vertebral endplate sclerosis and intervertebral disk degeneration. PMID: 27256073
  48. A Smad3-PTEN regulatory loop controls proliferation and apoptotic responses to TGF-beta in mouse endometrium. PMID: 28524854
  49. TGFss through the Alk1/TgfssR2 receptor axis is acting on endothelial cells to produce hematopoiesis. PMID: 29253505
  50. The adoptive transfer of NK1.1(-) CD4(+) NKG2D(+) cells suppressed DSS-induced colitis largely dependent on TGF-beta. Thus, NK1.1(-) CD4(+) NKG2D(+) cells exhibited immune regulatory functions, and this T cell subset could be developed to suppress inflammation in clinics. PMID: 28224733
  51. The present study describes the ability of color-coded intravital imaging to demonstrate the efficacy of a TGF-beta inhibitor to target stroma in an orthotopic mouse model of pancreatic cancer.The present study describes the ability of color-coded intravital imaging to demonstrate the efficacy of a TGF-beta inhibitor to target stroma in an orthotopic mouse model of pancreatic cancer. PMID: 28441080
  52. The ratio of full-length versus cleaved uPAR as analysed by Western blotting and its regulation was assessed by addition of different protease inhibitors and transforming growth factor - beta1 (TGF-beta1). The role of uPAR cleavage in cell proliferation and migration was analysed using real-time cell analysis and invasion was assessed using the myoma invasion model PMID: 28526008
  53. Hepatic Stellate Cells express iron-transport proteins. Holotransferrin (iron) activates HSC fibrogenesis and the TGF-beta pathway, whereas iron depletion by chelation reverses this, suggesting that this could be a useful adjunct therapy for patients with fibrosis. PMID: 29406047
  54. The results show that lnc-Spry1 could act as an early mediator of TGF-beta signaling and reveal different roles for a long noncoding RNA in modulating transcriptional and posttranscriptional gene expression. PMID: 28186499
  55. TIMAP is suppressed by TGFbeta via HDAC3-associated Smad signaling. PMID: 27709267
  56. AMTN mRNA levels were induced at the initiation of apoptosis by TGFbeta1, which mediated through the Smad3 bindings to AMTN gene promoter. PMID: 27502207
  57. these results demonstrated that endogenous tumor-derived TGF-beta aids in suppressing local anti-tumor immunity, resulting in tumor growth, whereas excess tumor-derived TGF-beta initially results in more rapid tumor proliferation and later growth suppression, independent of anti-tumor immunity PMID: 28855127
  58. Dynamic expression and regulatory mechanism of TGF-beta signaling in chicken embryonic stem cells differentiating into spermatogonial stem cells PMID: 28495881
  59. The results provide the first evidence that upregulation of TGFb/Smad3 in injured arteries induces local smooth muscle cells CXCR4 expression and cell migration, and consequently intimal hyperplasia. PMID: 27340942
  60. These results indicated that carnosic acid (CA)attenuated oxidative stress via inhibition of Nox4 expression in TGF-beta-stimulated fibroblasts and UUO operated-kidneys, suggesting that CA may be useful for the treatment of fibrosis-related diseases PMID: 27212017
  61. pattern of STAT indicates that possibly TGF-beta and IL-6 play a crucial role in differentiation of DCs subsets and Treg/Th17 imbalance during experimental cerebral malaria (ECM). PMID: 27632786
  62. Ltbp4 regulates Pdgfrb expression via TGFbeta-dependent modulation of Nrf2 transcription factor function. PMID: 27645114
  63. hese evidences collectively proved that the activation of IL0 and TGFB1 protected the host from antigen-induced asthma, possibly through IL10 signaling. This study shed some light on the modulations of IL10 and TGFB1, and related networks to asthma progression PMID: 27696149
  64. TGF-beta and beta-catenin crosstalk in proximal tubules may have a role in tubular injury in two models of chronic kidney disease PMID: 28701516
  65. Deletion of Tgfbr2 in myeloid cells leads to cerebrovascular inflammation and stroke with 100% penetrance. PMID: 29051340
  66. Expression of LAP/TGF-beta1, CD80, CD86 and PD-L1 is significantly increased in tumor-infiltrating B cells (TIL-B) relative to splenic B cells. LAP/TGF-beta1 expression on TIL-B progressively increased from 5.4+/-1.7% on day 8 to 43.1+/-6.1% by day 21 post tumor implantation. PMID: 26895637
  67. transforming growth factor (TGF)-beta-induced intracellular plasminogen activator inhibitor (PAI)-1 activation is responsible for keeping Hematopoietic stem and progenitor cells in the bone marrow niche. PMID: 28821477
  68. Following vasectomy, IL1alpha, IL1beta, IL1ra, IL10, and TNF-alpha may mediate immune reaction in whole epididymis, whereas IL6 and TGF-beta1 may mediate regionally different immune response primarily in the lower part of epididymis. PMID: 27476761
  69. plasticity of Group 3 innate lymphoid cells is regulated by the balance between the opposing effects of Notch and TGF-beta signaling, maintaining homeostasis in the face of continual challenges PMID: 27141930
  70. ur findings suggest that embryonic thymosphere-forming stem cells (TSFCs) contribute to an adult SC pool and that TSFC plasticity is controlled by TGF-b signaling. PMID: 27705793
  71. Data show that just like TGF-beta1, TGF-beta2 is expressed in and secreted by both, healthy and diseased hepatocytes and hepatic stellate cell (HSCs). PMID: 26799667
  72. FGF23 augments pro-fibrotic signalling cascades in injury-primed renal fibroblasts via activation of FGFR4 PMID: 28919046
  73. Mice with impaired Gq/11 signaling had reduced stretch-mediated generation of TGFbeta by epithelial cells and enhanced macrophage MMP12 synthesis but were protected from the effects of ventilator-induced lung injury. PMID: 27811142
  74. tissue engineered periosteum can deliver FGF-2, TGF-beta1, and ASCs to a mouse critical-sized femur defect and further optimization may yield improved bone allograft healing. PMID: 27874253
  75. TGFbeta stabilized the abundance of BRCA1 by reducing the abundance of microRNA-182 (miR-182). Ectopic expression of BRCA1 or antagonism of miR-182 in cultured TGFbeta-deficient mammary epithelial cells restored luminal lineage commitment. PMID: 27923913
  76. inhibition of TGFbeta signaling by the small molecule SB431542 potentiates calvarial skeletal repair through activation of bone morphogenetic protein (BMP) signaling on osteoblasts and dura mater cells participating in healing of calvarial defects. PMID: 27036931
  77. we evaluated the therapeutic effect of TGF-b-mediated in-vitro-differentiated MDSCs on colitis. Adoptive transfer of MDSCs that differentiated with TGF-b led to better colitis prevention than the transfer of MDSCs that differentiated without TGF-b PMID: 28009291
  78. removing TGF-b type II receptor in DA neurons also disrupts the balance in TGF-b1 expression in DA neurons and neighboring GABAergic neurons, which increases inhibitory input, reduces excitatory synaptic input, and alters phasic firing patterns in DA neurons PMID: 28009292
  79. PAR1 in its inactive unligated state functions as a scaffold for TGFbetaRII to downregulate TGF-beta signaling, and thereby promote embryonic stem cell transition to functional endothelial cells. PMID: 27866874
  80. HSP22 functions as a negative regulator in the TGF-beta-stimulated migration of osteoblasts. PMID: 27396899
  81. Lnc-LFAR1 binds directly to Smad2/3 and promotes transcription of TGFbeta, Smad2, Smad3, Notch2 and Notch3 which, in turn, results in TGFbeta and Notch pathway activation. PMID: 28747678
  82. P311 is a novel TGFbeta1/Smad signaling-mediated regulator of transdifferentiation in epidermal stem cells during cutaneous wound healing. PMID: 27906099
  83. how that limiting CCR2(+) monocytic myeloid-derived suppressor cells accumulation reduces the pulmonary contents of TGF-beta1, TIMP-1 and collagen after silica treatment PMID: 28799208
  84. RORgamma mediates epithelial-mesenchymal transition of hepatocytes during hepatic fibrosis facilitated by TGFbeta1. PMID: 27791279
  85. these data unravel the previously unrecognized role of TGF-beta1 in promoting Treg viability, coinciding with the pronounced immunomodulatory role of these cells during later phase of oropharyngeal candidiasis infection PMID: 26530137
  86. findings define a novel physiological function of Shp2 in TGF-beta1/MMP12-dependent emphysema, adding insights into potential etiologies for this chronic lung disorder. PMID: 28814604
  87. TGF-beta and PPARalpha signaling pathways are involved in radiation-induced heart fibrosis, metabolic dysregulation, and impaired heart contractility, a pathophysiological condition that is often observed in patients that received high radiation doses in thorax. PMID: 27805817
  88. Data show that Clostridium difficile Toxin A induces TGF-beta1 signaling pathway activation and suggest that this pathway might play a protective role against the effect of C. difficile-toxin. PMID: 28784928
  89. These findings implicate TGF-beta-Smad2/3 signaling in activated tissue-resident cardiac fibroblasts as principal mediators of the fibrotic response. PMID: 28891814
  90. identified trihydroxyphenolic compounds as potent blockers of TGF-beta1 responses (IC50 ~50 nM), Snail1 expression, and collagen deposition in vivo in models of pulmonary fibrosis and collagen-dependent lung cancer metastasis. PMID: 28872461
  91. Study found that in endothelial cells thrombospondin-4 (TSP-4), a secreted extracellular matrix protein, is upregulated in response to TGF-beta1 and mediates the effects of TGF-beta1 on angiogenesis. PMID: 28481870
  92. a novel and intimate link between the protein kinase NDR1 and TGFbeta signaling PMID: 23840619
  93. the methyltransferase Set9 potentiates TGF-beta signaling by targeting Smad7, an inhibitory downstream effector. PMID: 27292644
  94. These results indicate that bidirectional communication between ovarian cancer (OvCa) cells and MC-derived CAFs, via TGF-beta-mediated MMT, seems to be crucial to form a suitable metastatic niche. We suggest MMT as a possible target for therapeutic intervention and a potential source of biomarkers for improving OvCa diagnosis and/or prognosis. PMID: 28247413
  95. Tfrc deletion dramatically suppressed both transforming growth factor-beta (TGF-beta) and bone morphogenetic protein (BMP) signaling in cranial neural crest cell-derived mandibular tissues. PMID: 27362800
  96. Results demonstrate the circRNA_010567/miR-141/TGF-beta1 axis plays an important regulatory role in the diabetic mice myocardial fibrosis model PMID: 28412345
  97. results show that Smads are directly required for Th cell differentiation independent of Runx induction but only indirectly required via Runx2 for TGF-beta1-induced selectin ligand induction on murine CD4 T cells PMID: 27543612
  98. TGF-beta1-induced CK17 enhances cancer stem cell-like properties rather than epithelial-mesenchymal transition in promoting cervical cancer metastasis via the ERK1/2-MZF1 signaling pathway. PMID: 28703907
  99. This study builds on previous studies that have implicated miR-17-92 in the regulation of important molecular components of the TGFB signaling pathway, it is likely that interactions remain to be elucidated between miR-17-92 and as-of-yet unidentified molecules important for the control of palatogenesis. PMID: 28662367
  100. these results suggest that IL-23 plays a critical role in regulation of Th17 cytokines. Furthermore, IL-6 and TGF-beta do not appear to influence IL-23-mediated restoration of Th17 cytokines after ethanol and burn injury. PMID: 28684599

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Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families TGF-beta family
Database Links

KEGG: mmu:21803

STRING: 10090.ENSMUSP00000002678

UniGene: Mm.248380

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