Tgfb1 Antibody

Datasheet
Code CSB-PA023446LA01MO
See More Details Free Antibody trial simple
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Uniprot No. P04202
Image
  • Western Blot
    Positive WB detected in: MCF-7 whole cell lysate
    All lanes: Tgfb1 antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 45 kDa
    Observed band size: 45 kDa

  • Immunoprecipitating Tgfb1 in MCF-7 whole cell lysate
    Lane 1: Rabbit control IgG instead of (1μg) instead of CSB-PA023446LA01MO in MCF-7 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA023446LA01MO (8μg) + MCF-7 whole cell lysate (500μg)
    Lane 3: MCF-7 whole cell lysate (10μg)

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol
Immunogen Recombinant Mouse Transforming growth factor beta-1 protein (30-278AA)
Raised in Rabbit
Species Reactivity Mouse, Human
Tested Applications ELISA, WB, IP; Recommended dilution: WB:1:500-1:5000, IP:1:200-1:2000
Relevance Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells (PubMed:18368049). Mediates SMAD2/3 activation by inducing its phosphorylation and subsequent translocation to the nucleus. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types (By similarity).
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Transforming growth factor beta-1 (TGF-beta-1) [Cleaved into: Latency-associated peptide (LAP)], Tgfb1
Immunogen Species Mouse
Research Area Cardiovascular
Target Names Tgfb1
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
References
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Function Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts
Subcellular Location Secreted, extracellular space, extracellular matrix
Protein Families TGF-beta family
Database Links

KEGG: mmu:21803

STRING: 10090.ENSMUSP00000002678

UniGene: Mm.248380

Pathway Hippo signaling pathway
MAPK signaling pathway
TGF-beta signaling pathway
Cell cycle
Cellular senescence
Osteoclast differentiation
Th17 cell differentiation
AGE-RAGE signaling pathway in diabetic complications
FoxO signaling pathway

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