Recombinant Danio rerio G1/S-specific cyclin-D1 (ccnd1)

Code CSB-YP820871DIL
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Source Yeast
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Code CSB-EP820871DIL
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Source E.coli
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Code CSB-EP820871DIL-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP820871DIL
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Source Baculovirus
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Code CSB-MP820871DIL
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ccnd1
Uniprot No.
Alternative Names
ccnd1; cycd1G1/S-specific cyclin-D1
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-291
Target Protein Sequence
MEHQLFCCEV DTIRRAYQDS NLLNDRVLQT MLKAEENYLP SPNYFKCVQK EIVPKMRKIV ATWMLEVCEE QKCEEEVFPL AMNYLDRFLS VEPTKKTRLQ LLGATCMFLA SKMKETVPLT AEKLCIYTDN SVRPGELLQM ELLALNKLKW DLASVTPHDF IEHFLAKLPI HQSSKQILRK HAQTFVALCA TDVNFIASPP SMIAAGSVAA AVQGLYLKST DSCLSSQNLT NFLSQVIRSD PDCLRSCQEQ IESLLESSLR QAQQHISTET KRVEEDVDLS CTPTDVRDIN I
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals.
Gene References into Functions
  1. Methylparabens exposure increased malformations, LPO, apoptosis, ccnd1 and myca expressions, and decreased GST activities and NO levels compared with the control group. PMID: 29360218
  2. while cyclin B1 RNA granules were disassembled in a manner dependent on actin filament depolymerization, certain fractions of mos RNA granules were disassembled independently of actin filaments. These results suggest that cytoplasmic regulation of translationally repressed mRNAs by formation of different RNA granules is a key mechanism for translational control of PMID: 27756483
  3. show that the knockdown of smc1a in zebrafish impairs neural development, increases apoptosis, and specifically down-regulates Ccnd1 levels PMID: 26206533
  4. Reduction of cyclin D1 expression compromises zebrafish eye and head development. PMID: 16284195
  5. Role in cell cycle control is mediated by meis1 regulating cyclin D1 and c-myc transcription in the embryonic eye. PMID: 18216175

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
Cyclin family, Cyclin D subfamily
Database Links
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