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| Code | CSB-EP009411HU |
| Size | $1812 |
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| Have Questions? | Leave a Message or Start an on-line Chat |
| Description |
The preparation of Recombinant Human GHR protein included 3 main steps: construct the expression vector, expression of protein of interest, and protein purification. Every step was performed under a strict QC system so that we got the premium protein. This GHR was expressed in E.coli at and fused with N-terminal 6xHis-SUMO tag. According to SDS-PAGE, the purity turns out to be 90%+. GHR belongs to a large family of cytokine or hematopoietic receptors. It activates signal transductors (JAK-2/STAT), the cascade of the mitogen-activated protein kinase (MAPK) and the phosphoinositide 3-kinase (PI3K) which are important for cell growth and survival. Recently, GHR is reported to be associated with cancer development and progression, including breast cancer and hepatocellular carcinoma. In addition, GHR relates to breast cancer chemoresistance and metastasis that GHR knockdown decreases the chemoresistant and metastatic behavior of estrogen receptor negative breast cancer. It was found that sorafenib could stimulate GHR expression in HCC cell lines, while other drugs including regorafenib, lenvatinib, and cabozantinib had no effects on GHR expression. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Target Names | GHR |
| Uniprot No. | P10912 |
| Research Area | Neuroscience |
| Alternative Names |
GH receptor; GH-binding protein; GHBP; GHBP, included; GHR; GHR_HUMAN; Growth hormone binding protein; Growth hormone receptor; Growth hormone receptor precursor; Growth hormone-binding protein; Growth hormone-binding protein, included; Increased responsiveness to growth hormone, included; Serum binding protein; Serum-binding protein; Somatotropin receptor
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| Species | Homo sapiens (Human) |
| Source | E.coli |
| Expression Region | 19-264aa |
| Target Protein Sequence | FSGSEATAAILSRAPWSLQSVNPGLKTNSSKEPKFTKCRSPERETFSCHWTDEVHHGTKNLGPIQLFYTRRNTQEWTQEWKECPDYVSAGENSCYFNSSFTSIWIPYCIKLTSNGGTVDEKCFSVDEIVQPDPPIALNWTLLNVSLTGIHADIQVRWEAPRNADIQKGWMVLEYELQYKEVNETKWKMMDPILTTSVPVYSLKVDKEYEVRVRSKQRNSGNYGEFSEVLYVTLPQMSQFTCEEDFY Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 44.4kDa |
| Protein Length | Extracellular Domain |
| Tag Info |
N-terminal 6xHis-SUMO-tagged |
| Form |
Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Troubleshooting and FAQs |
Protein FAQs |
| Storage Condition | Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | 3-7 business days |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |
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| Function |
Receptor for pituitary gland growth hormone involved in regulating postnatal body growth. On ligand binding, couples to the JAK2/STAT5 pathway.; The soluble form (GHBP) acts as a reservoir of growth hormone in plasma and may be a modulator/inhibitor of GH signaling.; Isoform 2 up-regulates the production of GHBP and acts as a negative inhibitor of GH signaling.
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| Gene References into Functions |
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| Involvement in disease | Laron syndrome (LARS); Growth hormone insensitivity, partial (GHIP) |
| Subcellular Location | Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein. |
| Protein Families | Type I cytokine receptor family, Type 1 subfamily |
| Tissue Specificity | Expressed in various tissues with high expression in liver and skeletal muscle. Isoform 4 is predominantly expressed in kidney, bladder, adrenal gland and brain stem. Isoform 1 expression in placenta is predominant in chorion and decidua. Isoform 4 is hig |
| Database Links |
HGNC: 4263 OMIM: 143890 KEGG: hsa:2690 STRING: 9606.ENSP00000230882 UniGene: Hs.125180 |
