Name: Recombinant Human Hyaluronidase PH-20 (SPAM1)
Brand: CUSABIO
SKU: CSB-EP022471HU

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

SPAM1

Uniprot No.

P38567

Research Area

Cancer

Alternative Names

epididymis secretory sperm binding protein Li 96n; HEL-S-96n; HYA1; HYAL PH-20; HYAL PH20; Hyal-PH20; HYAL1; HYAL3; HYAL5; HYALP_HUMAN; Hyaluronidase PH-20; Hyaluronoglucosaminidase PH-20; MGC26532; PH-20 Hyaluronidase; PH20; PH20 Hyaluronidase; SPAG15; SPAM-1; Spam1; sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding); Sperm adhesion molecule 1; Sperm surface protein PH-20; Sperm surface protein PH20; zona pellucida binding

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

36-490aa

Target Protein Sequence

LNFRAPPVIPNVPFLWAWNAPSEFCLGKFDEPLDMSLFSFIGSPRINATGQGVTIFYVDRLGYYPYIDSITGVTVNGGIPQKISLQDHLDKAKKDITFYMPVDNLGMAVIDWEEWRPTWARNWKPKDVYKNRSIELVQQQNVQLSLTEATEKAKQEFEKAGKDFLVETIKLGKLLRPNHLWGYYLFPDCYNHHYKKPGYNGSCFNVEIKRNDDLSWLWNESTALYPSIYLNTQQSPVAATLYVRNRVREAIRVSKIPDAKSPLPVFAYTRIVFTDQVLKFLSQDELVYTFGETVALGASGIVIWGTLSIMRSMKSCLLLDNYMETILNPYIINVTLAAKMCSQVLCQEQGVCIRKNWNSSDYLHLNPDNFAIQLEKGGKFTVRGKPTLEDLEQFSEKFYCSCYSTLSCKEKADVKDTDAVDVCIADGVCIDAFLKPPMETEEPQIFYNASPSTLS
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

67.9kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombinant Human Hyaluronidase PH-20(SPAM1) is a partial length protein expressed with N-terminal 6xHis-SUMO-tagged in the E.coli. Its expression region corresponds to 36-490aa of human SPAM1 protein. Its purity was determined by SDS-PAGE and reached up to 90% and presented a molecular mass band of 67.9 kDa on the gel. This recombinant SPAM1 protein may be used to synthesize antibodies against SPAM1.
SPAM1 is involved in sperm-egg adhesion. Upon fertilization, sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid. Diseases associated with SPAM1 include Mucopolysaccharidosis, Type Ix and Aqueous Misdirection.

Customer Reviews and Q&A

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■ Q&A

Please send me a quotation for the following inquiry.
I want to Mammalian cell expression for CHO-S cell.
I am trying to make a cloning in the HZ24 plasmid containing 5 NheI and 3 BamHI restriction sites.
Please refer to below link that is desired protein sequence.
www.genome.jp/dbget-bin/www_bget?dr:D06604
The patents in the link below shows how cloning and expression are performing in full details.
https://patents.google.com/patent/KR101493644B1/ko
It cannot be necessarily the same, just refer to reference, and trying to make active PH20 soluble for expression after glycosylation.

Very nice to receive your inquiry. Your specified sequence is only a few amino acids shorter than the mature full length protein, so we recommend to express the full length of the mature protein.
Recombinant Human Hyaluronidase PH-20(SPAM1)
CSB-EP022471HU >> E.coli
You can view the full information of sequence and tag on this page.
Sorry, we didn't check the corresponding product on R&D website, in theory, the protein expressed by the Mammalian cell system should have the post-translational modification,however, we haven't done any validation.
Please remark your requirement for tag removal if you need the untagged protein or tag removal when placing the order.
Sorry, we haven't tried to remove the tag before. We can try enzyme digestion, but we can't guarantee 100% successfully.
The overall success rate of enzyme digestion data analysis is 75%-86%.
Not all protein tags can be removed as some proteins will be very unstable after tag removal.
If we succeed in removing the tag, we will charge for extra cost.
If we fail in removing the tag, we won’t charge for tag removal and provide the fusion protein, and remark this information in datasheet as follows
“Note: The laboratory determined that the Tag on your protein could not be removed with standard laboratory procedures. Your protein is being supplied with the Tag intact.”
Generally, the delivery time will be extended for 3 days.

Target Background

Function

Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid.

Gene References into Functions

Human posterior head 20 (hPH20) and homo sapiens sperm acrosome associated 1 (hSPACA1) immunocontraceptive epitopes reduced fertility in male/female mice. PMID: 25209425
results indicate that neither rHuPH20 nor its directly generated HA catabolites have inflammatory properties in the air pouch model, and rHuPH20 can instead inhibit some aspects of inflammation, such as neutrophil infiltration into the air pouch. PMID: 24778442
HSPA2 regulates the expression of sperm surface receptors involved in human sperm-oocyte recognition, such as arylsulfatase A and SPAM1. PMID: 23247813
The interaction between SPAM1, ARSA and HSPA2 in a multimeric complex mediating sperm-egg interaction. PMID: 23209833
PH20 is elevated in demyelinating lesions and that increased PH20 expression is sufficient to inhibit oligodendrocyte precursor cell maturation and remyelination. PMID: 23463525
SPAM1 mRNA & protein occur in all 3 regions of epididymis & vas deferens. It has hyaluronidase activity at pH 7.0. The proximal promoter has epididymal transcription factor sites including androgen receptor elements. Its role may be in sperm maturation. PMID: 12932297

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Protein Families

Glycosyl hydrolase 56 family

Tissue Specificity

Testis.

Database Links

HGNC: 11217

OMIM: 600930

KEGG: hsa:6677

STRING: 9606.ENSP00000345849

UniGene: Hs.121494

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