The gene encoding the human MMP9 (107-707aa) was cloned into a plasmid and expressed in yeast cells as an N-terminal fusion to a 6xHis tag. The recombinant human MMP9 protein is harvested from the culture supernatant of the transformed yeast cells and purified through affinity chromatography. Its purity reaches over 90% as assessed by SDS-PAGE.
Human MMP9 plays a crucial role in degrading the extracellular matrix (ECM) during normal tissue remodeling [1][2]. MMP9 degrades basement membrane proteins in the ECM [6]. Elevated levels of MMP9 have been associated with various pathological conditions, including chronic inflammation and different types of cancers such as oral, breast, and renal cell carcinomas [3][4][5]. Furthermore, MMP9 has been found to modulate tight junction integrity and cell viability in human airway epithelia, affecting epithelial health and barrier function.
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