Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

TYK2

Uniprot No.

P29597

Research Area

Signal Transduction

Species

Homo sapiens (Human)

Source

Baculovirus

Expression Region

589-875aa

Target Protein Sequence

ITQLSHLGQGTRTNVYEGRLRVEGSGDPEEGKMDDEDPLVPGRDRGQELRVVLKVLDPSHHDIALAFYETASLMSQVSHTHLAFVHGVCVRGPENIMVTEYVEHGPLDVWLRRERGHVPMAWKMVVAQQLASALSYLENKNLVHGNVCGRNILLARLGLAEGTSPFIKLSDPGVGLGALSREERVERIPWLAPECLPGGANSLSTAMDKWGFGATLLEICFDGEAPLQSRSPSEKEHFYQRQHRLPEPSCPQLATLTSQCLTYEPTQRPSFRTILRDLTRLQPHNLA
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

34.4 kDa

Protein Length

Partial

Tag Info

N-terminal 10xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

In the process of expressing the recombinant Human TYK2 protein, baculovirus cells are transfected with a DNA expression vector that carries the gene encoding the desired protein (589-875aa). The cells are then cultured to induce the expression of the intended protein. The protein features a N-terminal 10xHis tag fusion. The recombinant Human TYK2 protein is collected and purified from the cell lysate through affinity purification, with its purity surpassing 85%, as assessed by SDS-PAGE.

Target Background

Function

Non-receptor kinase involved in various processes including cell growth, development, cell migration, innate and adaptive immunity. Plays both structural and catalytic roles in numerous cytokines and interferons signaling. Associates with cytokine and growth factor receptors and activate STAT family members including STAT1, STAT3, STAT4 or STAT6. The heterodimeric cytokine receptor complexes are composed of a TYK2-associated receptor chain (IFNAR1, IL12RB1, IL10RB or IL13RA1) which serves as the signal transducing chain harboring STAT docking sites once phosphorylated by TYK2, and a second receptor chain associated either with JAK1 or JAK2. In turn, recruited STATs are phosphorylated, form homo- and heterodimers, translocate to the nucleus, and regulate cytokine/growth factor responsive genes. Negatively regulates STAT3 activity by promototing phosphorylation at a specific tyrosine that differs from the site used for signaling.

Gene References into Functions

New ELAVL1-TYK2 fusion gene is oncogenic in acute myeloid leukemia. PMID: 27189703
TYK2 gene variants do not seem to play an important role in Chagas disease susceptibility and/or chronic Chagas cardiomyopathy PMID: 29304122
TYK2 polymorphism is not associated with Dermatomyositis /Polymyositis in the Chinese Han population PMID: 28846454
TYK2 activating mutants and fusion proteins were detected in patients diagnosed with leukaemic diseases suggesting that TYK2 is a potent oncogene. PMID: 26631911
TYK2 genetic variants are differentially associated with common autoimmune diseases PMID: 27807284
High TYK2 expression is associated with B-cell lymphoma. PMID: 28716895
These data indicate that germline activating TYK2 mutations predispose to the development of ALL. PMID: 27733777
TYK2 rs280519 genotype was significantly related to severe necroinflammatory activity (NIA) grade of chronic hepatitis C patients. PMID: 28704535
TYK2 is associated with systemic sclerosis susceptibility and reinforce the relevance of the IL-12 pathway in SSc pathophysiology. PMID: 26338038
Clinical genomic analysis of the current series of NF1-MPNST cases found that TYK2 is a new gene mutated in MPNST. PMID: 27875628
Results demonstrates that the TYK2 rs2304256 and rs12720356 polymorphisms are associated with susceptibility to rheumatic diseases, rs2304256 polymorphism is associated with systemic lupus erythematous (SLE) in Caucasians, and rs280519 polymorphism is associated with SLE in Caucasians and Asians. [meta-analysis] PMID: 26980740
demonstration of the single marker association of tyrosine kinase-2 polymorphisms with ulcerative colitis and Crohn's disease in Turkish population. PMID: 25744728
The TYK2 promoter variant is associated with an overall risk for diabetes. PMID: 26288847
Genetic associations and gene-gene interactions of IRF5 and TYK2 were significantly detected in Han Chinese with systemic lupus erythematosus PMID: 26294277
The majority of disease-associated mutations in JAKs map to a pseudokinase domain (JH2), demonstrating its central regulatory function. PMID: 26359499
the role of TYK2 in the pathogenesis of rheumatoid arthritis PMID: 25849893
present findings suggest that TYK2 regulates apoptotic and proinflammatory pathways in pancreatic beta-cells via modulation of IFNalpha signaling, subsequent increase in MHC class I protein, and modulation of chemokines such as CXCL10. PMID: 26239055
Tyrosine Kinase 2-mediated Signal Transduction in T Lymphocytes Is Blocked by Pharmacological Stabilization of Its Pseudokinase Domain. PMID: 25762719
Data indicate a chimeric fusion involving nucleophosmin NPM1 (5q35) and TYK2 kinase (19p13) that encodes an NPM1-TYK2 protein in cutaneous CD30 antigen-positve lymphoproliferative disorders. PMID: 25349176
Immune cell dysfunction may contribute to enhancement and maintenance of immune inflammatory and autoimmune responses by production of pathogenic cytokines and immune complexes. The role of Tyk2 in innate and adaptive immune cells is discussed. Review. PMID: 24654603
In cellular assays, SAR-20347 dose dependently (1 nM-10 muM) inhibited JAK1- and/or TYK2-dependent signaling from the IL-12/IL-23, IL-22, and IFN-alpha receptors. PMID: 25156366
TYK2 as a novel associated locus for idiopathic inflammatory myopathies. PMID: 24812289
Here, we report the 2.8-A structure of the two-domain pseudokinase-kinase module from the JAK family member TYK2 in its autoinhibited form. PMID: 24843152
The crystal structure of a receptor-binding fragment of human TYK2, encompassing the FERM and SH2 domains, in complex with peptide motif from the interferon-alpha receptor chain 1 (IFNAR1) is presented. PMID: 24704786
Our results indicate that the biological processes that require TYK2 catalytic function in humans are restricted to the IL-12 and IL-23 pathways. PMID: 23894201
TYK2 rs34536443 polymorphism is associated with a decreased susceptibility to endometriosis-related infertility. PMID: 23000200
Two rare autoimmune disease-associated Tyk2 variants are catalytically impaired but signaling competent. PMID: 23359498
The rs6445975 polymorphism of phox homology domain containing serine/threonine kinase and the rs2304256 polymorphism of tyrosine kinase 2 are associated with the development of systemic lupus erythematosus in Europeans PMID: 22592861
The most associated variant in primary biliary cirrhosis was in chromosome 19, a low-frequency nonsynonymous single nucleotide polymorphism in TYK2. PMID: 22961000
In our pilot study, we discovered significant changes in methylation patterns of genes IL-7, IL-13, IL-17C and TYK2 between henodialysis patients and healthy subjects PMID: 22506826
This study demonistrated that Rs55762744 is a rare variant of modest effect on multiple sclerosis risk affecting a subset of patients. PMID: 22744673
Studied the doubly tagged full-length construct, H6-FL-TYK-2-FLAG. In the presence of ATP and a peptide substrate, H6-FL-TYK-2-FLAG showed a marked lag in phosphopeptide product formation. TYK-2 KD showed no such lag. PMID: 22486643
data highlight the role of TYK2 downregulation in breast cancer cell de-differentitation and initiation of regional metastasis PMID: 22116632
Association analysis identified five SLE susceptibility genes reaching genome-wide levels of significance : NCF2 ,IKZF1 ,IRF8 ,IFIH1 , and TYK2 PMID: 22046141
infection of airway epithelial cells with hMPV decreased cellular level of Janus tyrosine kinase (Jak1) and tyrosine kinase 2 (Tyk2) PMID: 21949722
This meta-analysis demonstrates that autoimmune and inflammatory diseases is associated with TYK2 gene rs34536443 and rs2304256 polymorphisms, but not rs280523, rs280519, rs12720270 and rs12720356. PMID: 21140222
The consequent effect of SOCS1 inhibition of Tyk2 not only results in a reduced IFN response because of inhibition of Tyk2 kinase-mediated STAT signaling but also negatively impacts IFNAR1 surface expression, which is stabilized by Tyk2. PMID: 21757742
Data suggest that TYK2 polymorphisms were not associated with systemic lupus erythematosus in Hong Kong Chinese, but that rs2304256 and rs12720270 may be associated with photosensitivity and discoid rash. PMID: 21196586
The rs34536443 variant effect on multiple sclerosis susceptibility might be mediated by deviating T lymphocyte differentiation toward a Th2 phenotype. PMID: 21354972
Tyk2 deficiency and clinical manifestations of hyper IgE syndromes (Review) PMID: 21178271
Siva-1 forms a functional complex with Tyk2 and participates in the transduction of signals that inhibit B lymphocyte growth. PMID: 20727854
Here, the authors report the crystal structures of TYK2, a first in class structure, and JAK3 in complex with PAN-JAK inhibitors CP-690550 and CMP-6, both of which bind in the ATP-binding cavities of both JAK isozymes. PMID: 20478313
Single-nucleotide polymorphism in TYK2 gene is confirmed to be associated with multiple sclerosis. PMID: 19888296
rs2304256 associated with increased risk of discoid lupus erythematosus PMID: 19758313
Studies indicate that the highest T1D association was at marker rs2304256 (odds ratio (OR) = 0.86; 95%CI = 0.82-0.90) in the TYK2 gene 19p13.2See TYK2 gene at chromosome 19p13.2. PMID: 19966805
Data show that Tyk2 tyrosine kinase is essential for stable cell surface expression of IFNAR1. PMID: 12554654
evidence that the small GTPases RhoA and Rac1, but not Cdc42, are directly associated with Tyk2 and PI3-K in an uPA/uPAR-dependent fashion and are necessary to mediate the uPA/uPAR-directed migration via the Tyk2/PI3-K signalling complex in human VSMC PMID: 12719789
Catalytically active Tyk2 is necessary for Janus kinase 2 phosphorylation and association with the platelet-activating factor receptor. PMID: 14500680
In the IFN signaling pathway leading to STAT activation, both JAK1 and TYK2 are essential, whereas NF-kappaB activation requires only TYK2. PMID: 15883164
mutations in the JAK1 and Tyk2 genes may be identified as initial molecular defects in human cancers and autoimmune diseases PMID: 16239216

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Involvement in disease

Immunodeficiency 35 (IMD35)

Protein Families

Protein kinase superfamily, Tyr protein kinase family, JAK subfamily

Tissue Specificity

Observed in all cell lines analyzed. Expressed in a variety of lymphoid and non-lymphoid cell lines.

Database Links

HGNC: 12440

OMIM: 176941

KEGG: hsa:7297

STRING: 9606.ENSP00000264818

UniGene: Hs.75516

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Code	CSB-BP025391HU5
Abbreviation	Recombinant Human TYK2 protein, partial
MSDS	MSDS Datasheet
Size	$528
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Non-receptor tyrosine-protein kinase TYK2 (TYK2), partial

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