Recombinant Mouse Cochlin (Coch)

Code	CSB-YP717267MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP717267MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP717267MO-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP717267MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP717267MO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

Coch

Uniprot No.

Q62507

Alternative Names

CochCochlin; COCH-5B2

Species

Mus musculus (Mouse)

Expression Region

27-552

Target Protein Sequence

EGAV PIPVTCFTRG LDIRKEKADV LCPGGCSLEE FSVFGNIVYA SVSSICGAAV HRGVIGTSGG PVRVYSLPGR ENYSSVDANG IQSQMLSRWS ASFAVTKGKS STQEATGRAV STAHPPSGKR LKKTPEKKTG NKDCKADIAF LIDGSFNIGQ RRFNLQKNFV GKVALMLGIG TEGPHVGLVQ ASEHPKIEFY LKNFTSAKDV LFAIKEVGFR GGNSNTGKAL KHTAQKFFTA DTGVRKGIPK VVVVFIDGWP SDDIEEAGIV AREFGVNVFI VSVAKPIPEE LGMVQDVAFV DKAVCRNNGF FSYHMPNWFG TTKYVKPLVQ KLCTHEQMMC SKTCYNSVNI AFLIDGSSSV GDSNFRLMLE FVSNIAKTFE ISDIGAKIAA VQFTYDQRTE FSFTDYNTKE NVLAVLANIR YMSGGTATGD AIAFTVRNVF GPIRDSPNKN FLVIVTDGQS YDDVRGPAAA AHDAGITIFS VGVAWAPLDD LRDMASKPKE SHAFFTREFT GLEPIVSDVI RGICRDFLES QQ

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Plays a role in the control of cell shape and motility in the trabecular meshwork.

Gene References into Functions

accumulation of acellular deposits in the incudomalleal and incudostapedial joints in Coch (G88E/G88E) mice similar to those found in human DFNA9-affected temporal bones PMID: 25049087
study reports that cochlin is expressed by follicular dendritic cells (FDCs) and selectively localized in the fine extracellular network of conduits in the spleen and lymph nodes; by producing cochlin, FDCs contribute to the innate immune response in defense against bacteria PMID: 23684986
Loss of Cochlin is associated with reduced intraocular pressure and mechanosensing. PMID: 22496787
In Coch knock-in/knockout mouse models of sensorineural hearing loss and vestibular dysfunction vestibular function is compromised before cochlear function. PMID: 21073934
Up-regulation of cochlin is an important etiologic factor leading to Usher syndrome type 1F. PMID: 20097680
Areas that express COCH mRNA as determined by in situ hybridization, and to the regions of the inner ear which show histological abnormalities in autosomal dominant sensorineural deafness and vestibular disorder, DFNA9. PMID: 11709536
gene expression in glaucomatous trabecular meshwork PMID: 15579465
Cochlin was found in the trabecular meshwork of very young mice, prior to elevated intraocular pressure, suggesting that over time the protein may contribute to the events leading to increased IOP and optic nerve damage--{REVIEW} PMID: 16332271
introduced the G88E mutation by gene targeting into the mouse and have created a Coch(G88E/G88E) mouse model for the study of DFNA9 pathogenesis and cochlin function PMID: 18697796