Recombinant Mouse Pyruvate kinase PKM(Pkm)

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Code CSB-EP018072MO
Size US$2466Purchase it in Cusabio online store
(only available for customers from the US)
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP018072MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Pkm.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP018072MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Pkm.
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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names Pkm
Uniprot No. P52480
Research Area Signal Transduction
Alternative Names Pkm; Pk3; Pkm2; PykmPyruvate kinase PKM; EC 2.7.1.40; Pyruvate kinase muscle isozyme
Species Mus musculus (Mouse)
Source E.coli
Expression Region 1-531aa
Target Protein Sequence MPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNTGIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPVP
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 62.8 kDa
Protein Length Full Length
Tag Info N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Data

Function Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation (By similarity).
Gene References into Functions
  1. PKM2 promotes tumor cell exosome release via phosphorylating protein SNAP23. PMID: 28067230
  2. PKM1 interacts with A-Raf, an upstream regulator of the MEK/ERK pathway, and that this interaction contributes to MEK1 phosphorylation by A-Raf. PMID: 28743549
  3. infiltrated/activated neutrophils at wound site release pyruvate kinase M2 (PKM2) by its secretive mechanisms during early stages of wound repair; the released extracellular PKM2 facilitates early wound healing by promoting angiogenesis at wound site PMID: 26808610
  4. The results suggested that targeting PKM2 with an oncolytic adenovirus produced a strong antitumor effect. PMID: 28569774
  5. PKM2 activation may protect against DN by increasing glucose metabolic flux, inhibiting the production of toxic glucose metabolites and inducing mitochondrial biogenesis to restore mitochondrial function. PMID: 28436957
  6. our results demonstrate that PKM2 is required to support metabolic reprogramming for homocysteine-induced B cell activation and function PMID: 27903739
  7. Jmjd8 is upregulated during endothelial differentiation and regulates endothelial sprouting and metabolism by interacting with pyruvate kinase M2. PMID: 27199445
  8. this study identifies a new signaling pathway for CD43 through the regulation of alternative functions of pyruvate kinase isoform M2, favoring cell survival following activation PMID: 27606486
  9. results show that the Aha1-Hsp90-PKM2/HIF-1alpha axis mediates the induction of aromatase in Li-Fraumeni Syndrome. PMID: 27467582
  10. in addition to its role in cancer metabolism, PKM2 plays a role in controlling systemic metabolic homeostasis and inflammation, thereby preventing hepatocellular carcinoma by a non-cell-autonomous mechanism. PMID: 27125672
  11. Evidence That Does Not Support Pyruvate Kinase M2 (PKM2)-catalyzed Reaction as a Rate-limiting Step in Cancer Cell Glycolysis. PMID: 26917721
  12. PKM2 promotes cell proliferation and insulin secretion, and inhibits apoptosis in pancreatic beta cells via Wnt/CTNNB1 signaling. PMID: 26823761
  13. Using [(32)P]-phosphoenolpyruvate (PEP) we examine the direct substrates of PKM2 using recombinant enzyme and in vitro systems where PKM2 is genetically deleted. The findings argue against a role for PKM2 as a protein kinase. PMID: 26300261
  14. LPS-induced PKM2 enters into a complex with Hif-1alpha, which can directly bind to the IL-1beta promoter, an event that is inhibited by activation of PKM2. PMID: 25565206
  15. observed a shift in the enzyme pyruvate kinase from the adult M1 (PKM1) isoform to the fetal M2 (PKM2) isoform, a hallmark of the Warburg Effect, in the heart after sunitinib treatment PMID: 25735978
  16. Muller glia compensate for their unique metabolic adaptations by using lactate and aspartate from neurons as surrogates for their missing PK and AGC1. PMID: 25313047
  17. PKM1 expression promotes a metabolic state that is unable to support DNA synthesis and cell proliferation. PMID: 25482511
  18. Up-regulation of PKM2 might protect intestinal epithelial cells against apoptosis possibly through Bcl-xl in Crohn's disease. PMID: 24817408
  19. This study provides the first evidence linking natural mutations in PKM2 with cancer. PMID: 24492614
  20. Our data reveal a complex interaction between STAT6 and PPARgamma in the regulation of liver and adipose tissue lipid depot distribution and design STAT6 as a novel link between inflammatory cell metabolism and adipocyte and hepatocyte function. PMID: 23917405
  21. PKM2 activity in beta-cells is oscillatory and are consistent with pulsatile PFK1 being the mediator of slow glycolytic oscillations. PMID: 24100037
  22. These data suggest that regulation of pyruvate kinase M2 isoform activity supports the different metabolic requirements of proliferating and nonproliferating tumor cells. PMID: 24120138
  23. PKM2 expression induces changes in type 1 fibers associated with muscle atrophy and muscle weakness in myotonic dystrophy type 1. PMID: 23901116
  24. PPARgamma contributes to PKM2 and HK2 expression in fatty liver PMID: 22334075
  25. Components of the mTOR/HIF1alpha/Myc-hnRNPs/PKM2 glycolysis signaling network could be targeted for the treatment of cancer caused by an aberrant RTK/PI3K/AKT/mTOR signaling pathway PMID: 21325052
  26. histidine-phosphorylated PGAM1 correlated with expression of PKM2 in cancer cell lines; decreased pyruvate kinase activity in PKM2-expressing cells allows PEP-dependent histidine phosphorylation of PGAM1 and may provide an alternate glycolytic pathway PMID: 20847263
  27. splicing repressors hnRNP A1 and A2, as well as the polypyrimidine-tract-binding protein PTB, contribute to control of pyruvate kinase isoform M1 and M2 expression PMID: 20133837
  28. Findings suggest that tyrosine phosphorylation regulates PKM2 to provide a metabolic advantage to tumor cells, thereby promoting tumor growth. PMID: 19920251
  29. study showed IL-3-induced nuclear translocation of the M2 isoform of pyruvate kinase (M2-PK) and examined its significance in cell proliferation [pyruvate kinase M2 isoform] PMID: 17446165
  30. results demonstrate that M2 expression is necessary for aerobic glycolysis and that this metabolic phenotype provides a selective growth advantage for tumour cells in vivo PMID: 18337823
  31. These findings suggest that M2 pyruvate kinase is a metabolic sensor which regulates cell proliferation, cell growth and apoptotic cell death in a glucose supply-dependent manner. PMID: 19563799

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Subcellular Location Cytoplasm, Nucleus
Protein Families Pyruvate kinase family
Tissue Specificity Embryonic stem cells and embryonal carcinoma cells.
Database Links

KEGG: mmu:18746

STRING: 10090.ENSMUSP00000034834

UniGene: Mm.326167

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