Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
NGF; NGFB; Beta-nerve growth factor; Beta-NGF; Fragment
Expression Region
110-229aa
Target Protein Sequence
SSSHPVFHRGEFSVCDSVSVWVGDKTTATDIKGKEVMVLGEVNINNSVFKQYFFETKCRDPNPVDSGCRGIDSKHWNSYCTTTHTFVKALTMDGKQAAWRFIRIDTACVCVLSRKAGRRA
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 110-229 constitute the expression domain of recombinant Pig NGF. The expected molecular weight for the NGF protein is calculated to be 29.4 kDa. This NGF recombinant protein is manufactured in e.coli. Fusion of the N-terminal 6xHis-SUMO tag into the NGF encoding gene fragment was conducted, allowing for easier detection and purification of the NGF protein in subsequent stages.
Pig beta-nerve growth factor (NGF) is a neurotrophic factor crucial for neuronal survival, development, and maintenance. It promotes neurite outgrowth, regulates synaptic plasticity, and influences neurotransmitter release. In neurobiology, NGF is vital for studying neuronal differentiation and neurodegenerative diseases. Its role in pain modulation makes it relevant in analgesic research. Additionally, NGF is implicated in immune responses and tissue repair. In regenerative medicine, NGF holds potential for nerve regeneration therapies. Studying NGF provides insights into neurotrophin biology, neuroregeneration, and pain modulation, offering avenues for therapeutic interventions in neurological disorders and pain management, as well as understanding its broader implications in immune function and tissue repair.
