Recombinant Probable protein E4(E4)

Code CSB-YP325294HNS
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Source Yeast
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Code CSB-EP325294HNS
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Source E.coli
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Code CSB-EP325294HNS-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP325294HNS
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Source Baculovirus
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Code CSB-MP325294HNS
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names E4
Uniprot No. P22421
Alternative Names E4; Protein E4
Species Human papillomavirus type 47
Expression Region 1-304
Target Protein Sequence MTKMKQMLIC ILCGHLCITW IQMMCGIRQQ VGSIKLAFTT YMEHLNTIMC YLLMMQRDIV LLENGKLKLI RKLCLLLSLA PHHQGHQEDK QTQTPPPRPP PPPQPPLTPR PDANPSINSH NKPKPNEEGT DGDHQAEQGD RKRTKGDPDP DPGRGPVLKP TLPPPPPPPP TGPGLRRSTR LVLVPGQGPP PDLPAPPVEG EVEGHPQGKD RDHPPPTPQN GHGKETQGAE GGGDKGEQGA VGGESSDGEG DHSQPPLTPP NESDGSLLNT VACLLARWES NFDQLVQNIQ GDLEGYWRKL GTPQ
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Contributes to multiple aspects of the viral life cycle including viral genome amplification, suppression of suprabasal cell differentiation and egress of newly formed virions. Induces host cell cycle arrest at the G2 phase by associating with and preventing the nuclear entry of host CDK1/cyclin B1 complexes. Inhibits cellular DNA replication by preventing loading of host replication licensing proteins MCM2 and MCM7 onto chromatin. Within the cytoplasm, associates with host kinase SRPK1, a splicing factor regulator, and inhibits its activity. Therefore, E4 favors expression of late viral transcripts by inhibiting SRPK1-mediated phosphorylation of host serine-arginine (SR) proteins that have critical roles in mRNA metabolism. Late in the infectious cycle, E4 also acts to diminish the integrity of the keratinocyte by disrupting the keratin cytoskeleton and inducing apoptosis through alteration of mitochondrial function to facilitate egress of the newly formed virions.
Subcellular Location Host cytoplasm. Host nucleus.
Protein Families Papillomaviridae E4 protein family
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Address
7505 Fannin St. Ste 610-312, Houston, TX 77054, USA
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