Recombinant Rat G1/S-specific cyclin-D1(Ccnd1)

Code CSB-YP004811RA
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Source Yeast
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Code CSB-EP004811RA
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Source E.coli
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Code CSB-EP004811RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP004811RA
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Source Baculovirus
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Code CSB-MP004811RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Ccnd1
Uniprot No. P39948
Alternative Names Ccnd1G1/S-specific cyclin-D1
Species Rattus norvegicus (Rat)
Expression Region 1-295
Target Protein Sequence MEHQLLCCEV ETIRRAYPDT NLLNDRVLRA MLKTEETCAP SVSYFKCVQR EIVPSMRKIV ATWMLEVCEE QKCEEEVFPL AMNYLDRFLS LEPLKKSRLQ LLGATCMFVA SKMKETIPLT AEKLCIYTDN SIRPEELLQM ELLLVNKLKW NLAAMTPHDF IEHFLSKMPE ADENKQIIRK HAQTFVALCA TDVKFISNPP SMVAAGSVVA AMQGLNLGSP NNFLSCYRTT HFLSRVIKCD PDCLRACQEQ IEALLESSLR QAQQNIDPKA TEEEGEVEEE AGLACTPTDV RDVDI
Protein Length Full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex, Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity).
Gene References into Functions
  1. autophagic degradation machinery and cyclin D1 linked to liver tumorigenesis PMID: 29328502
  2. In this report we examine the roles of two RBPs that bind to AU-rich elements (ARE) - AUF1 and HuR - in the stability and translation of cyclin D1 (Ccnd1) mRNA in rat myoblasts transiting the G phase of the cell cycle, and their interactions with miRNAs PMID: 30207314
  3. the experimental results show that changes in the expression of cyclin D1 and CDK4 may accelerate apoptosis of amygdala neurons in PTSD-like rats. PMID: 28983608
  4. study shows that cyclin D1 gene silencing suppresses proliferation and induces apoptosis of rat chondrocytes in IL-1beta-induced osteoarthritis. PMID: 28548679
  5. The discovery of the downregulation of miR-26b in pulmonary artery remodeling, as well as its regulation of its target genes, CTGF and CCND1, may have important implications in our understanding of the molecular mechanisms underlying PAH, and may also lead to the development of new therapeutic interventions in this devastating and life-threatening disease. PMID: 27322082
  6. expression of miR-17 was significantly lower, whereas the expression of Cyclin D1 was significantly higher in glioma C6 cells compared to normal brain tissue. MiR-17 mimics decreased the viability and migration of glioma C6 cells markedly at 48 h. In addition, MiR-17 inhibitor increased the viability and migration of glioma C6 cells at 24 and 48 h PMID: 29351283
  7. these data demonstrated that Dixdc1 might participate in Schwann cell proliferation by targeting CyclinD1 and p21 at least partially through the PI3K/AKT activation. PMID: 27521891
  8. These results highlight the essential role of NF-kappaB-dependent transcriptional upregulation of cyclin D1 in EGFR-mediated cytoprotective effects under hypoxic preconditioning. PMID: 27443256
  9. AGO1 and AGO2 play non-equivalent roles of in mRNA turnover and translation of Ccnd1 mRNA. PMID: 26846850
  10. The robust increase in the expression of cyclin D1 and CDK4 at day 2 suggests that Muller glia may be in G1 phase of the cell cycle at this stage. PMID: 26975029
  11. Glucagon phosphorylates serine 552 of beta-catenin leading to increased expression of Ccnd1 and c-Myc in the isolated rat liver. PMID: 26135564
  12. a critical role for cyclin D1 dynamics in phenotype switching during glioma differentiation PMID: 26188547
  13. MSCs therapy promotes hepatocyte proliferation and prolongs survival in SFSLT by reducing ischemia reperfusion injury and acute inflammation, and sustaining early increased expressions of c-Jun N-terminal Kinase, Cyclin D1, and NF-kappaB. PMID: 25479410
  14. Treatment of non-transformed ileal crypt cells with PKC agonists has a biphasic effect on cyclin D1 expression. Initial PKCalpha-mediated down-regulation is followed by recovery and accumulation of cyclin to levels higher than those seen in untreated cells. PMID: 24914206
  15. we identified a new MicroRNA, miR-365, involving in the pathological process of vascular injury, which inhibits VSMC proliferation through targeting cyclinD1 PMID: 24936138
  16. Data show that up-regulation of cyclin D1 by hepatitis B virus X protein (HBx) in oval Cells ss dependent of the activation of MEK/ERK and PI-3K/Akt signaling pathways. PMID: 24577313
  17. during myogenesis, CCND1 transcription is activated bt the phosho-CRTC2-CREB complex. PMID: 25047835
  18. TGF-beta1 Smad signaling is involved in hexachlorobenzene-induced alterations of p27 and cyclin D1 protein levels. PMID: 25093615
  19. Plasmid-based shRNA against cyclin D1 ameliorated pulmonary vascular remodeling in monocrotaline-induced pulmonary hypertension. PMID: 23948595
  20. Intermedin/adrenomedulin 2 promotes renal cell proliferation and regeneration after renal hypoxia-reperfusion injury by upregulating cyclin D1 via MAPK signalling pathways. PMID: 23782291
  21. Cyclin D1 mRNA expression and cyclin D1(+) /MyoD(+) cell numbers significantly increased in cultures treated with 1 ng/ml IL-6. PMID: 23869758
  22. Bromodichloromethane decreases E-cadherin mRNA and increases that of c-myc and cyclin D1 in kidneys. PMID: 24001804
  23. Dietary Chlorella was found to diminish beta-catenin and cyclin D1 mRNA in colon tissues exposed to 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine. PMID: 23466459
  24. IGF-I upregulates cyclin D1 transcription partially by inducing cAMP response element binding protein phosphorylation through the ERK-MAP kinase pathway, and thus increasing its recruitment to the cyclin D1 promoter. PMID: 23447091
  25. Data show that the MEK-ERK pathway regulates cell cycle-related neuronal apoptosis (CRNA) by elevating the levels of cyclin D1, and the increase in cyclin D1 attenuates the activation of cyclin-dependent kinase 5 (cdk5) by its neuronal activator p35. PMID: 22833568
  26. Jiangu granule containing serum increase the mRNA and protein expressions of Cyclin D1 and CDK4, and inhibit P21 expression in cultured osteoblasts. PMID: 21179739
  27. Co-overexpression of Akt and Ang-1 in MSC activated cell cycle progression by upregulation of miR-143 and stimulation of FoxO1 and Erk5 signaling. PMID: 22374674
  28. The results of this study suggested that cyclin D1 may be a putative target for the development of neuroprotective strategies sparing physiological synaptic NMDAR signaling. PMID: 22354185
  29. study demonstrates that integrin-ECM interaction collaborates with mitogens to activate ERK/mitogen-activated protein kinase pathways which are essential for cyclin D1 expression in podocytes PMID: 22678010
  30. Behavioral experiments suggested that overexpression of Ccnd1 produces a deficit in learning and memory. PMID: 21982980
  31. YY1 inhibited p21WAF1/Cip1 complex formation with cdk4 and cyclin D1 to prevent tumor growth. PMID: 22322893
  32. CCN2 contributes to the cigarette smoke extract and nicotine-induced proliferation of pulmonary artery smooth muscle cells at least in part by upregulating cyclin D1 expression. PMID: 21928352
  33. cAMP/Rap1B/B-Raf pathway modulates the expression of p27 and the cytoplasmic mislocalization of p27-cyclin D1 in tuberous sclerosis gene-regulated-renal cancer. PMID: 21693435
  34. CTGF and cyclin D1 expressions significantly were upregulated in pulmonary arteries from rats exposed to cigarette smoke, and may be associated with abnormal proliferation of pulmonary artery smooth muscle cells. PMID: 21092636
  35. CSE induced rat PASMCs proliferation at least partly via PKCalpha-mediated cyclin D1 expression. PMID: 21465534
  36. Oviductus Ranae treatment can lower the expressions of Cyclin D1 and CDK6 in the liver to enhance the liver cell proliferation in aged male rats. PMID: 20501390
  37. The present study revealed a gender difference in MNNG-induced rat gastric carcinogenesis involving the sex difference in cyclin D1/cdk4 expression. PMID: 21628965
  38. Data show that proliferaton and nuclear expression of cyclin D1 and p-Rb coincide with the early phase of cyst growth, suggesting that there might be a therapeutic window in which cyclin-dependent kinase inhibitors are most effective in ARPKD. PMID: 20924203
  39. These findings suggest that the low expression of beta-catenin and cyclin D1 may reduce the activity of epidermal stem cells from diabetic rats. PMID: 21468580
  40. Cyclin D1 might play a critical role in cigarette smoke-induced pulmonary vascular remodeling via regulating pulmonary artery smooth muscle cell proliferation. PMID: 20227424
  41. Our data suggest that beta-catenin is an important downstream molecule in the PI3-Akt-GSK3beta pathway, and as such it mediates IGF-I upregulation of cyclin D1 mRNA and promotion of cell proliferation and survival in oligodendroglial cells. PMID: 20235220
  42. KLF5 is a downstream signal of the ERK 1/2 and p38 MAPK pathways, and activates the transcription of cyclin D1 gene via functional interaction with c-Jun in Ang II-induced VSMC proliferation. PMID: 20037604
  43. These findings propose that hypoxia increases cyclin D1 expression through activation of JNK in neural stem cells (NSCs)of rat in vitro, suggesting a novel possible mechanism for hypoxia-induced proliferation of NSCs. PMID: 19909792
  44. cyclin D1 is a novel ligand-independent co-repressor PMID: 12048199
  45. cyclin D1 is critically regulated by extracellular stimuli that control hepatocyte proliferation PMID: 12085346
  46. Results show that cyclin D1 and CDK4 have a role in hypertrophic growth of cardiomyocytes through a novel mechanism(s) which may not be related to its role in cell cycle progression. PMID: 12163013
  47. Data show that the steady-state expression of p21(waf1/cip1) and cyclin D1 is influenced by the methylation status of their 5'-flanking regions upon development of diabetes. PMID: 12373555
  48. Anaysis of response elements that contribute to up-regulation of cyclin D1 gene expression. PMID: 12504100
  49. data show that c-Jun-N-terminal kinase (JNK) drives G0 to G1 transition in hepatocytes and that cyclin D1 is a downstream target of the JNK pathway during liver regeneration PMID: 12668975
  50. cyclin D1 is a key target of amino acid signaling in hepatocytes PMID: 12736274

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Subcellular Location Nucleus, Cytoplasm, Membrane
Protein Families Cyclin family, Cyclin D subfamily
Database Links

KEGG: rno:58919

STRING: 10116.ENSRNOP00000028411

UniGene: Rn.22279

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