Product Details

Purity

>85% (SDS-PAGE)

Target Names

Ikbkb

Uniprot No.

Q9QY78

Alternative Names

Ikbkb; IkkbInhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; EC 2.7.11.10; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB

Species

Rattus norvegicus (Rat)

Expression Region

1-757

Target Protein Sequence

MSWSPSLPTQ TCGAWEMKER LGTGGFGNVI RWHNQVTGEQ IAIKQCRQEL SPKNRDRWCL EIQIMRRLNH PNVVAARDVP EGMQNLAPND LPLLAMEYCQ GGDLRRYLNQ FENCCGLREG AILTLLSDIA SALRYLHENR IIHRDLKPEN IVLQQGEKRL IHKIIDLGYA KELDQGSLCT SFVGTLQYLA PELLEQQKYT VTVDYWSFGT LAFECITGFR PFLPNWQPVQ WHSKVRQKSE VDIVVSEDLN GTVKFSSSSP FPNNLNSVLA ERLEKWLQLM LTWQPRQRGV DPQYGPNGCF RALDDILNLK LVHILNMVTG TIHTYPVMED ESLQSLKTRI REDTGILETD QELLQEAGLV LLPDKPATQC ISDSKTNEGL TLDMDLVFLF DNSKMSYETQ ITPRPQPESV SCVLQEPKRN LSFFQMRKVW GQVWHSIQTL KEDCNRLQQG QRAAMMNLLR NNSCLSKMKN AMASTAQQLK AKLDFFKTSI QIDLEKYREQ TEFGITSDKL LLAWREMEQA VEQCGRENDV KVLVERMMAL QTDIVDLQRS PMGRKQGGTL DDLEEQAREL YRRLREKPRD QRTEGDSQDM VRLLLQAIQS FEKKVRVIYS QLSKTVVCKQ KALELLPKVE EVVRLMNEDE KTVVRLQEKR QKELWNLLKI ACSKVRGPVS GSPDSMNVSR LSHPGHLMSQ PSSACDSLPD SDKKSEELVA EAHALCSRLE SALQDTVKQQ DRSFTTLDWS WLQMEDEERC GLEQACD

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE. IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs. Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor. Also phosphorylates other substrates including NCOA3, BCL10 and IRS1. Within the nucleus, acts as an adapter protein for NFKBIA degradation in UV-induced NF-kappa-B activation. Phosphorylates RIPK1 at 'Ser-25' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death. Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus.

Gene References into Functions

Inhibition of IkappaB kinase at peak creatinine in acute kidny injury improves functional recovery, reduces further injury, and prevents fibrosis. PMID: 28673900
Data indicate that injury-induced upregulation of inflammatory cytokines within IVDs and increased levels of neuropeptides within DRG neurons can be suppressed by inhibiting IkappaB kinase-beta (IKKbeta). PMID: 24825147
IKKbeta could reduce arterial remodeling events by affecting both the inflammatory and the growth-promoting response of Ang II. PMID: 24135021
Selective inhibition of IkappaBbeta-mediated NF-kappaB activation maintains the inflammatory stress-induced upregulation of antioxidant enzyme and preserves cellular growth, as shown in fetal pulmonary vascular endothelial cells. PMID: 23418625
Following treatment with polymixin B, I-kappaB kinase mRNA expression was downregulated in pulmonary alveolar macrophages. PMID: 12857501
down-regulation of the NF-kappaB pathway via IKKbeta inhibition dampened the chronic inflammatory process associated with rat adjuvant-induced arthritis [IKKbeta] PMID: 17009244
With the prolongation of smoking duration, the phosphorylation of IKK-ss in type 2 diabetic rats increased. After smoking cessation, the phosphorylation of IKK-ss decreased. PMID: 20646420
Report the IkappaB kinase inhibitor nuclear factorkappaB essential modulator-binding domain peptide inhibits injury-induced neointimal formation. PMID: 20930169
data suggest that NGF-induced PC12 differentiation includes activation of IKK2 which may promote the release of c-Rel-containing dimers PMID: 15935065
canonical IKKbeta-dependent NF-kappaB activation is inhibited by peroxynitrite, which also activates NF-kappaB-inducing kinase and IKKalpha phosphorylation PMID: 16079150
dominant negative IKK2-Treg is a unique population of CD4CD25 T cells expressing high levels of inducible nitric oxide synthase. PMID: 17318081
both IKKalpha and IKKbeta are necessary and sufficient for physiological muscle atrophy PMID: 18827022
differences in glucose tolerance between rats with a low capacity for running and rats with a high capacity to run are at least partly explained by differences in IKKbeta activity and pSer (307) levels PMID: 19569009

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Subcellular Location

Cytoplasm. Nucleus. Membrane raft.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family, I-kappa-B kinase subfamily

Database Links

KEGG: rno:84351

STRING: 10116.ENSRNOP00000025851

UniGene: Rn.19222

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Recombinant Rat Inhibitor of nuclear factor kappa-B kinase subunit beta (Ikbkb)

Product Details

Customer Reviews and Q&A

Target Background

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