Recombinant Takifugu rubripes Interleukin-6 (il6)

Code CSB-YP760907TKY
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Source Yeast
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Code CSB-EP760907TKY
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Source E.coli
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Code CSB-EP760907TKY-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP760907TKY
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Source Baculovirus
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Code CSB-MP760907TKY
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
il6
Uniprot No.
Alternative Names
il6; Interleukin-6; IL-6
Species
Takifugu rubripes (Japanese pufferfish) (Fugu rubripes)
Expression Region
23-227
Target Protein Sequence
APLDAPTE SPAGETSGEE AETGSPDDAL AVALESVLGA TKLHKNEFLV EFQGEVKYDF LDRYKIPSLP AKCPYSNFGK DACLRRLLEG LLIYSVLLKR VEEEFPSSSI LSEVRFYSNI LIKELENKVR DRDQVMRLTS SQEEQLLKDT DYPDTFHRKM TAHGILYNLH YFLVDCRRVI NKRAKHRESA GSRVVRAVTF YHPKKRS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway. The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells.
Gene References into Functions
  1. These results suggest that fugu IL-6 regulates antibody production through STAT3 signaling via IL-6R and gp130. PMID: 22469658
Subcellular Location
Secreted.
Protein Families
IL-6 superfamily
Tissue Specificity
After induction, highly expressed in spleen. Can also be expressed in kidney after incubation with PHA.
Database Links
CUSABIO guaranteed quality
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