ACVR2B Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA260702A0HU
Size	US$210
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Image	IHC image of CSB-RA260702A0HU diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA260702A0HU diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Target Background

Product Details

Uniprot No.

Q13705

Target Names

ACVR2B

Alternative Names

Activin receptor type-2B (EC 2.7.11.30) (Activin receptor type IIB) (ACTR-IIB), ACVR2B

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human Activin Receptor Type IIB

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7B10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Activin receptor type-2B (ACVR2B) serves as a critical transmembrane serine/threonine kinase receptor within the TGF-β superfamily signaling network. This receptor mediates cellular responses to activins, myostatin, and related ligands, playing essential roles in muscle homeostasis, metabolic regulation, and developmental processes. For researchers investigating signal transduction pathways or exploring stem cell biology, reliable detection of ACVR2B expression provides valuable insights into these fundamental biological mechanisms.

This recombinant monoclonal antibody, generated from clone 7B10 in rabbit host, offers the reproducibility advantages inherent to recombinant technology. Because the antibody sequence is defined and expressed consistently, researchers can expect uniform performance across experiments and between lots, eliminating the variability concerns that can complicate long-term studies or multi-site collaborations. The antibody targets a synthetic peptide derived from human ACVR2B, ensuring specificity for this receptor in human samples.

Validation studies demonstrate robust performance in immunohistochemistry applications, with successful staining confirmed in paraffin-embedded human breast cancer tissue and human placenta tissue. Both validations employed citrate buffer antigen retrieval at pH 6.0 under high pressure conditions, with the antibody performing effectively at 1:100 dilution following overnight incubation at 4°C. The recommended working range of 1:50 to 1:200 provides flexibility for optimization across different tissue types and detection systems. Additional compatibility with ELISA extends the antibody's utility for quantitative applications.

Supplied in a glycerol-containing buffer with BSA stabilizer, this affinity-purified antibody is well-suited for researchers studying ACVR2B-mediated signaling in cancer biology, reproductive tissue development, or stem cell differentiation contexts.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Transmembrane serine/threonine kinase activin type-2 receptor forming an activin receptor complex with activin type-1 serine/threonine kinase receptors (ACVR1, ACVR1B or ACVR1c). Transduces the activin signal from the cell surface to the cytoplasm and is thus regulating many physiological and pathological processes including neuronal differentiation and neuronal survival, hair follicle development and cycling, FSH production by the pituitary gland, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. Activin is also thought to have a paracrine or autocrine role in follicular development in the ovary. Within the receptor complex, the type-2 receptors act as a primary activin receptors (binds activin-A/INHBA, activin-B/INHBB as well as inhibin-A/INHA-INHBA). The type-1 receptors like ACVR1B act as downstream transducers of activin signals. Activin binds to type-2 receptor at the plasma membrane and activates its serine-threonine kinase. The activated receptor type-2 then phosphorylates and activates the type-1 receptor. Once activated, the type-1 receptor binds and phosphorylates the SMAD proteins SMAD2 and SMAD3, on serine residues of the C-terminal tail. Soon after their association with the activin receptor and subsequent phosphorylation, SMAD2 and SMAD3 are released into the cytoplasm where they interact with the common partner SMAD4. This SMAD complex translocates into the nucleus where it mediates activin-induced transcription. Inhibitory SMAD7, which is recruited to ACVR1B through FKBP1A, can prevent the association of SMAD2 and SMAD3 with the activin receptor complex, thereby blocking the activin signal. Activin signal transduction is also antagonized by the binding to the receptor of inhibin-B via the IGSF1 inhibin coreceptor.

Gene References into Functions

the A allele of genetic variant rs2276541 in ACVR2B is associated with lean muscle mass PMID: 26848890
ActR-IIB is expressed in male germ cells and Sertoli cells. PMID: 26289399
We found that the AAAAA, AGGAG, and AGGGA haplotypes in ACVR2B were associated with susceptibility to Premature Ovarian Failure when they also had at least one CATAG haplotype in ADAMTS19. PMID: 25051287
miR-21 interacts directly with the 3'-untranslated region of ACVR2B mRNA. Mechanical stretch suppressed ACVR2B protein levels in periodontal ligament stem cells. Gain- and loss of function of ACVR2B mediated the osteogenic differentiation of PDLSCs. PMID: 25203845
Activin type IIB receptors are clearly demonstrable throughout the adult human hypothalamus and basal forebrain. PMID: 22296042
After eccentric exercise, postmenopausal women not using hormone therapy (HT) expressed lower levels of ActRIIb while postmenopausal women using HT showed a heightened response. PMID: 22395277
The interaction between all five miRNAs and ACVR2B was verified by an in vitro assay. PMID: 22431721
Mutations in Activin A Receptor Type IIB were identified in 4 of the 47 patients (8.5%) with heterotaxy syndrome. Our results expand the mutation spectrum of monogenic heterotaxy syndrome with associated cardiac anomalies. PMID: 21864452
discussion of crystal structure of kinase domain of ActRIIB; structural analysis may be of help in developing selective inhibitors [REVIEW] PMID: 21353874
Inhibition of negative regulators of skeletal muscle by a soluble form of activin type IIB receptor (ACE-031) increases muscle mass independent of fiber-type expression. PMID: 20466801
analysis of expression and cellular compartmentalization of the activin receptors ActRIIA, ActRIIB and ActRIB, the inhibin co-receptor (betaglycan), and activin signaling proteins Smads 2, 3 and 4, and growth regulatory role during lactation PMID: 12782414
activin signaling via type II receptors requires a specific sequence for ALK4 binding PMID: 15123686
These results indicate that haplotype structure at the ACVR2B and follistatin loci may contribute to interindividual variation in skeletal muscle mass and strength, although these data indicate sex-specific relationships. PMID: 17347381
The high-resolution structure of human ActRIIB kinase domain in complex with adenine establishes the conserved bilobal architecture consistent with all other catalytic kinase domains. PMID: 17893364

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Involvement in disease

Heterotaxy, visceral, 4, autosomal (HTX4)

Subcellular Location

Cell membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, TKL Ser/Thr protein kinase family, TGFB receptor subfamily

Database Links

HGNC: 174

OMIM: 602730

KEGG: hsa:93

STRING: 9606.ENSP00000340361

UniGene: Hs.174273

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The overview of TGF-β signaling pathway Do You Really Know TGF-β Superfamily? ACVR2A and ACVR2B as Key Targets for Muscle Growth, Weight Loss, PHA in Drug Research！ MSTN: A Key Target for Targeted Therapy in Muscle Diseases Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

TGF-beta signaling pathway Signaling pathways regulating pluripotency of stem cells Target Therapy

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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