CDKN2A Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA976457A0HU
Size	US$210
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Image	Immunocytochemical staining of Hela cells with Cyclin dependent kinase inhibitor 2A antibody. Nuclei were stained blue with DAPI; Cyclin dependent kinase inhibitor 2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm. Western blotting analysis using Cyclin dependent kinase inhibitor 2A antibody. Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Cyclin dependent kinase inhibitor 2A antibody and HRP-conjugated goat anti-rabbit secondary antibody respectively.
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Product Details
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Target Background

Product Details

Uniprot No.

Q8N726

Target Names

CDKN2A

Alternative Names

CDKN2A; Cyclin Dependent Kinase Inhibitor 2A; P14ARF; CDK4I; MTS1; ARF; Cyclin-Dependent Kinase 4 Inhibitor A; P16-INK4A; P16INK4A; CDKN2; CMM2; INK4; P16; P19; P14; MLM; Cyclin-Dependent Kinase Inhibitor 2A (Melanoma, P16, Inhibits CDK4); Cyclin-Dependent Kinase Inhibitor 2A; Multiple Tumour Suppressor 1; Multiple Tumor Suppressor 1; CDKN2A/ARF Intron 2 LncRNA; Alternative Reading Frame; Inhibitor Of Cdk4 A; P19ARF; INK4A; MTS-1; CAI2; Cell Cycle Negative Regulator Beta; P19 Alternate Open Reading Frame; P14 Alternate Open Reading Frame; CDK4 Inhibitor P16-INK4; Tumor Suppressor ARF; P16-INK4a; P16INK4a; P16-INK4; P16INK4; P19Arf; INK4a; TP16

Species Reactivity

Human

Immunogen

Recombinant Human CDKN2A protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

8B7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, ICC

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
ICC	1:100-1:1000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Isoform smARF may be involved in regulation of autophagy and caspase-independent cell death; the short-lived mitochondrial isoform is stabilized by C1QBP.

Subcellular Location

Nucleus, nucleolus. Nucleus, nucleoplasm.; [Isoform smARF]: Mitochondrion.

Database Links

HGNC: 1787

OMIM: 600160

UniGene: Hs.512599

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Related pathways

p53 signaling pathway Cell cycle Cellular senescence

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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