HSP90AA1 Antibody

Code CSB-RA011087A0HU
Size US$350
Image
  • Western Blot
    Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissue
    All lanes: Hsp90 alpha antibody at 0.8μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 85, 99 KDa
    Observed band size: 85 KDa

  • IHC image of CSB-RA011087A0HU diluted at 1:80 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-RA011087A0HU diluted at 1:80 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of NIH/3T3 cells with CSB-RA011087A0HU at 1:26, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

  • Immunoprecipitating Hsp90 in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-RA011087A0HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-RA011087A0HU (3μg) + Hela whole cell lysate (500μg)
    Lane 3: Hela whole cell lysate (20μg)

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Product Details

Uniprot No. P07900
Target Names HSP90AA1
Alternative Names Heat shock 86 kDa antibody; Heat shock protein 90kDa alpha cytosolic class A member 1 antibody; Heat shock protein 90kDa alpha cytosolic class B member 1 antibody; Heat shock protein HSP 90 alpha antibody; Heat shock protein HSP 90 beta antibody; Heat shock protein HSP 90-alpha antibody; HS90A_HUMAN antibody; HSP 84 antibody; HSP 86 antibody; Hsp 90 antibody; HSP86 antibody; HSP90A antibody; HSP90AA1 antibody; HSP90AB1 antibody; HSP90B antibody; HSPC1 antibody; HSPC2 antibody; HSPCAL1 antibody; HSPCAL4 antibody; Renal carcinoma antigen NY-REN-38 antibody
Species Reactivity Human, Rat
Immunogen A synthesized peptide derived from human HSP90AA1
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Monoclonal
Isotype Rabbit IgG
Purification Method Affinity-chromatography
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Form Liquid
Tested Applications ELISA, WB, IHC, IF, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:200
IF 1:20-1:200
IP 1:200-1:1000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Data

Function Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity which is essential for its chaperone activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function
Gene References into Functions
  1. RPAP3 provides a flexible scaffold for coupling HSP90 to the human R2TP co-chaperone complex. PMID: 29662061
  2. The study shows that a conserved tryptophan in the middle domain senses the interaction of Hsp90 with a stringent client protein and transfers this information via a cation-pi interaction with a neighboring lysine. PMID: 29662162
  3. While activation in c-Src is strictly controlled by ATP-binding and phosphorylation, the authors find that activating conformational transitions are spontaneously sampled in Hsp90-dependent Src mutants. PMID: 28290541
  4. chemotherapy agents can induce HSP90AA1 expression in osteosarcoma cells. And HSP90AA1, acting as an important regulator of autophagy, is a critical factor in the development of osteosarcoma chemoresistance both in vitro and in vivo. HSP90AA1 provides a novel therapeutic target for improving osteosarcoma treatment. PMID: 30153855
  5. We confirm that miR-628-3p promotes apoptosis and inhibits migration in A549 cells by negatively regulating HSP90. Our results may reveal a novel strategy for lung cancer treatment PMID: 29888262
  6. Data recognize HSP90 as a novel binding partner of PKM2 in hepatocellular carcinoma (HCC) cells. HSP90 potentiates the glycolysis and proliferation, reduces the apoptosis and thus enhances the growth of HCC cells through PKM2 Thr-328 phosphorylation maintaining its stability. PMID: 29262861
  7. EGFR expression stratified most pronounced among HSP90low tumours, where the EGFRhigh phenotype was associated with longer survival PMID: 28765916
  8. the SGT1-HSP90 complex contributes to the E3 ligase activity of the CUL4A complex that is necessary for CENP-A ubiquitylation and CENP-A deposition at the centromere. PMID: 28816574
  9. our data suggested that Hsp90alpha could positively regulate the self-renewal of BCSCs by facilitating the nuclear translocation of c-Myc and EZH2 to maintain BMI1 expression. PMID: 28914785
  10. HSP90 contributes to cutaneous vasodilation via NOS-dependent mechanisms in young habitually active men during exercise in the heat. PMID: 28751373
  11. The association between the MEEVD C-terminal peptide from the heat shock protein 90 (Hsp90) and tetratricopeptide repeat A (TPR2A) domain of the heat shock organizing protein (Hop) is a useful prototype to study the fundamental molecular details about the Hop-Hsp90 interaction. Observed are conformational changes of the peptide and the protein receptor induced by binding. The binding free energy is 8.4 kcal/mol. PMID: 28723223
  12. Our findings demonstrate Hsp90 blockade leads to ICN1 destabilization, providing an alternative strategy to antagonize oncogenic Notch1 signaling with Hsp90-selective inhibitors PMID: 28143869
  13. Generated multiple mutant KRAS-driven cancer cell lines with acquired resistance to the purine-scaffold HSP90 inhibitor PU-H71. Report a Y142N missense mutation in the ATP-binding domain of HSP90alpha that co-occurred with amplification of the HSP90AA1 locus in resistant cells. PMID: 28032595
  14. ATM is the primary kinase responsible for phosphorylation of Hsp90alpha after exposure ionizing radiation. PMID: 27738310
  15. molecular modeling was employed to incorporate experimental data using partial constructs of the Hsp90 C-terminal domain. PMID: 27771574
  16. findings suggested that this mechanism may be exploited by the Hsp90-Cdc37 chaperone to recruit and protect intrinsically dynamic kinase clients from degradation PMID: 29267381
  17. The findings establish an active role for Tsc1 as a facilitator of Hsp90-mediated folding of kinase and non-kinase clients-including Tsc2-thereby preventing their ubiquitination and proteasomal degradation. PMID: 29127155
  18. Data indicate HSP90 inhibitors as a class of preferred drugs for treatment combination with immunotherapy. PMID: 28878208
  19. Data suggest that SOCS3 is an important signaling protein in CLL, and Hsp90 inhibitors represent an approach to target transcriptional repression in B cell lymphoproliferative disorders. PMID: 27107422
  20. FKBP51 is primarily localized in mitochondria and hTERT is totally nuclear, upon the onset of oxidative stress, FKBP51 (but not FKBP52) becomes mostly nuclear colocalizing with hTERT, and longer exposure times to peroxide favors hTERT export to mitochondria. PMID: 27233944
  21. High HSP90 expression is associated with Colorectal Cancers. PMID: 28870917
  22. High HSP90 expression is associated with prostate cancer. PMID: 28038472
  23. Data suggest HSP90AA1-dependent regulation of ATM-NBN-CHK2 and ATR-CHK1 axes influences cells capability to repair double-stranded DNA damage; mechanisms include phosphorylation, polyubiquitination, and proteasomal degradation/proteolysis. (HSP90AA1 = heat shock protein 90kDa alpha; ATM = ataxia telangiectasia mutated protein; NBN = nibrin; CHK = checkpoint kinase; ATR = ataxia telangiectasia and Rad3 related kinase) PMID: 28631426
  24. Data show that pyruvate kinase M2 (PKM2) directly interacted with mutant growth factor receptor (EGFR) and heat-shock protein 90 (HSP90), and thus stabilized EGFR by maintaining its binding with HSP90 and co-chaperones. PMID: 26500058
  25. Binding of FM807 to the N-terminus of Hsp90 disrupted Hsp90/client complexes, resulting in degradation of the Hsp90 client protein EGFR and inhibition of the downstream pathway. PMID: 28157708
  26. Conventional as well as scaled molecular dynamics simulations further demonstrate that citrullination of selected Arg residues leads to progressive disruption of HSP90 tertiary structure, promoting exposure of R502/R510 to PAD modification and subsequent autoantibody binding. PMID: 27448590
  27. SYK is an HSP90 client protein, and B-cell receptor signaling-dependent phosphorylation of HSP90 on Y197 is required for this interaction. HSP90 promotes Burkitt lymphoma cell survival by maintaining tonic B-cell receptor signaling. PMID: 28064214
  28. Data indicate a chaperone function of nicotinamide mononucleotide adenylyl transferase 2 (NMNAT2), independent from its enzymatic activity, and NMNAT2 complexes with heat shock protein 90 (HSP90) to refold aggregated protein substrates. PMID: 27254664
  29. In the bound state, the Hsp90 dimer predominantly populates an open conformation, and transthyretin retains its globular structure. PMID: 28218749
  30. CD30 facilitates phosphorylation of heat shock factor 1, activates heat shock promoter element, and induces heat shock protein (HSP) 90. PMID: 27870927
  31. However, once the mumps virus L protein formed a mature polymerase complex with the P protein, Hsp90 activity was no longer required for the stability and activity of the L protein. PMID: 28053100
  32. HSP90 may be essential for stabilization and function of P2X7Rs through an action on the cysteine-rich domain of the cytoplasmic the C-terminus. PMID: 27301716
  33. HSP90AA1 and AB1 genes exhibit low expression in breast cancers highly sensitive to chemotherapy and may indicate the patients with higher probability of pathological complete response. PMID: 28051275
  34. the effect of HSP90 inhibition on IL-17-mediated cytokine and antimicrobial peptide expression in keratinocytes following heat treatment, was examined. PMID: 27279135
  35. epididymis secretory protein 4 had better specificity than CA125 in discriminating ovarian cancer, and endometrial cancer from benign gynecological diseases in southern China population PMID: 27302312
  36. Hsp90 has roles in the regulation of autophagy, such as toll-like receptor (TLR)-mediated autophagy, Ulk1-mediated mitophagy, and chaperone-mediated autophagy (CMA) [review] PMID: 26432328
  37. this study identified HSP90AA1 as a new potential biomarker for Behcet's disease by comparing highly ranked genes from all the built network-derived gene lists, which was confirmed the with real-world clinical samples PMID: 27226232
  38. Data show that heat shock protein 90 (HSP90) inhibitor 17-DMAG caused loss of ret proto-oncogene protein (RET) and proto-oncogene protein erbB-3 (ERBB3) phosphorylation and lead to rapid cell death. PMID: 26595521
  39. Hsp103 associates with cochaperone proteins, such as Hop, Cdc37 and Aha1, similar to Hsp90. The extra domain reduces the ATP hydrolysis when compared to Hsp90 thereby acting as a negative regulator of the chaperones intrinsic ATPase activity. PMID: 23951259
  40. Data suggest that synergistic mechanism between heat shock protein 90 (Hsp90) inhibitor SNX-7081 and fludarabine nucleoside (2-FaraA) may provide an alternative treatment for chronic lymphocytic leukemia (CLL) patients with p53 protein mutations. PMID: 26556860
  41. The expression of HSP90A was increased in the HCC cells, serum, and tissues. Immunohistochemistry analysis on 76 clinical tissue samples also suggested the relevance between HSP90A expression and HCC metastatic behavior. PMID: 26704341
  42. Aarsd1 inhibits the activity of a paradigmatic Hsp90 client protein. PMID: 26884463
  43. study confirmed Hsp90 as an influenza virus A PB2 polymerase interacting protein, and established that Hsp90 interacts with both the E627 and 627K variants, but has established this interaction is species independent, and both mammalian and avian Hsp90 can bind to the PB2 protein PMID: 26616658
  44. Data show that high-affinity heat shock protein 90 (HSP90) binding conferred by the inhibitor backbone could be exploited for conjugate accumulation within tumor cells. PMID: 26271675
  45. In conjunction with HSP90, the cytoplasmic USP19 may play a key role in triage decision for the disease-related polyQ-expanded substrates, suggesting a function of USP19 in quality control of misfolded proteins by regulating their protein levels PMID: 26808260
  46. The region of aa 250-295 of BGLF4 is essential for the BGLF4/Hsp90 interaction. PMID: 26982469
  47. The thermodynamics of binding of Cyp-40 to Hsp90 shows remarkable temperature sensitivity in the physiological temperature range. PMID: 26330616
  48. HSP90 overexpression is a prognostic marker for cholangiocarcinoma. HSP90-targeted therapy may be an option for a subset of cholangiocarcinoma. PMID: 26141945
  49. From our screening methodology, we identified HCAb2 as a breast tumor specific heavy chain antibody targeting cell surface heat shock protein 90. PMID: 26334999
  50. Heat shock protein 90 is required for ex vivo neutrophil-driven autoantibody-induced tissue damage in experimental epidermolysis bullosa acquisita. PMID: 25739426
  51. Studies indicate that the multiple roles of heat shock protein 90 (Hsp90) in the retina and the therapeutic potential of Hsp90 as a target in retinal disease. PMID: 26427407
  52. upon viral recognition, a direct connection between HSP90AA1 and the AKT-MTOR pathway trigger autophagy, a critical step for controlling infection PMID: 25714412
  53. The angiogenesis of human hepatocellular carcinoma may be mediated by HSP90; a specific HSP90 inhibitor, AUY922, has a therapeutic role in the treatment of hepatocellular carcinoma. PMID: 25955495
  54. leukocyte mRNA increased immediately after exercise following downhill running compared to flat running and in hot compared to temperate conditions PMID: 25722377
  55. YAP, TAZ and HSP90 take part in contact guidance and intercellular junction formation in corneal epithelial cells. PMID: 25290150
  56. Even if p23 predominantly binds the Hsp90 dimer, p23 is also able to interact with Hsp90 oligomers, shifting the Hsp90 dimer-oligomers equilibrium toward dimer. PMID: 26151834
  57. Our results validated HSP90 as an important target in pheochromocytoma and provided rationale for the testing of HSP90 inhibitors as a promising therapeutic agent in the antitumor therapy of pheochromocytoma. PMID: 25682284
  58. HSP90 regulates DNA repair by interaction between XRCC1 and DNA polymerase beta. PMID: 25423885
  59. Hsp90, by interacting with acetylated alpha-tubulin, plays a crucial role in viral capsid protein nuclear transport and may provide novel insight into the role of Hsp90 in HSV-1 infection. PMID: 24901434
  60. Expression, function and regulation of Hsp90aa1. [Review] PMID: 26071189
  61. plasma Hsp90alpha protein levels are useful as a diagnostic biomarker in lung cancer and predict the responses of patients with lung cancer to chemotherapy. PMID: 25316816
  62. RIP3 activation following the induction of necroptosis requires the activity of an HSP90 and CDC37 cochaperone complex. PMID: 25852146
  63. Data indicate that heat shock protein 90kDa (Hsp90) inhibition suppresses 26S proteasome remodeling, unanchored ubiquitin chain production, and aggresome clearance. PMID: 25713068
  64. Targeting oncoprotein stability via inhibiting HSP90 overcomes leukemic drug resistance caused by FLT3 kinase domain mutations. PMID: 24849514
  65. Level of TRX-1 in skeletal muscle tissue was lower while that of HSP90 was higher in type 2 diabetes than in impaired glucose tolerance subjects. PMID: 24689038
  66. Results suggest that heat shock protein 90 (Hsp90) inhibitors may overcome ligand-triggered resistance in microtubule associated protein-like 4 (EML4)--anaplastic lymphoma kinase (ALK) lung cancer cells. PMID: 24952482
  67. These results suggest that differences in the middle domain of Hsp90alpha and Hsp90beta may be responsible for the isoform-specific interactions with selected proteins. PMID: 25486457
  68. role for heat shock protein 90alpha in the proliferation and migration of HaCaT cells and in the deep second-degree burn wound healing in mice PMID: 25111496
  69. CDC37 has an important role in chaperoning protein kinases; it stabilizes kinase clients by a mechanism that is not dependent on a substantial direct interaction between CDC37 and HSP90, but requires HSP90 activity PMID: 24292678
  70. Ectopic expression of PRKD2 protected cancer cells from the apoptotic effects of HSP90 abrogation. PMID: 25297628
  71. This demonstration of combinatorial benefit afforded by an HSP90 inhibitor to a standard CRC. PMID: 24682747
  72. Data indicate that dual targeting of heat-shock proteins 90 (Hsp90) and BRAF(V600E) kinase provided combinatorial benefit in vemurafenib-sensitive melanoma cells in vitro and in vivo. PMID: 24398428
  73. Defect in HSP90 expression in highly differentiated human CD8(+) T lymphocytes. PMID: 24922078
  74. Data indicate that heat shock protein 90 (HSP90) expression was significantly higher in cyclin-dependent kinase inhibitor p16 (p16)-negative compared with p16-positive samples. PMID: 25205430
  75. data suggest a new paradigm for Hsp90-modulated assembly of a Cul3/DBC2 E3 ubiquitin ligase complex that may extend to other E3 ligase complexes. PMID: 24608665
  76. The elevated Hsp90alpha level at the end of heat stress and its positive signal in the cytoplasm of myocardial cells after heat stress could be associated with its protective role PMID: 24782094
  77. As a novel Hsp90 inhibitor, FW-04-806 binds to the N-terminal of Hsp90 and inhibits Hsp90/Cdc37 interaction, resulting in the disassociation of Hsp90/Cdc37/client complexes and the degradation of Hsp90 client proteins. PMID: 24927996
  78. Hsp10 and Hsp90 may be involved in large bowel carcinogenesis. PMID: 25075042
  79. The Photorhabdus toxins TccC3 and TccC5 interacted with Hsp90, FKBP51, Cyp40 and CypA, suggesting a role of these host cell factors in translocation and/or refolding of the ADP-ribosyltransferases. PMID: 24138221
  80. this study demonstrated that although ACT1-v2-D10N is nonfunctional, ACT1-v1-D19N retains the ability to interact with Hsp90 and is fully responsive to IL-17A stimulation. PMID: 25024377
  81. Authors have identified novel interactions between Chikungunya virus nsP3 or nsP4 proteins with the host stress-pathway chaperone HSP-90 protein. PMID: 24388965
  82. Dynamic interplay between hsp90, apo-sGC-beta1, and sGC-alpha1 in response to NO is unprecedented and represent new steps by which cells can modulate the heme content and activity of sGC for signaling cascades. PMID: 24733395
  83. Studies indicate that early clinical trials have confirmed the efficacy of heat shock protein 90 (Hsp90) inhibition as a targeted therapy in non-small-cell lung cancer (NSCLC). PMID: 24463348
  84. P53 facilitates stabilization of the closed Hsp90 dimer eliciting the fundamental stimulatory effect of Hsp90 by p53. PMID: 24191708
  85. The chaperone function of Hsp90 is sustained by Hsp72 and Hsp73, which contribute to the survival of myeloma cells. PMID: 23065523
  86. Hsp90alpha was highly expressed in leukemia cells and the expression level of Hsp90alpha was associated with leukemia prognosis. PMID: 24680776
  87. a monoallelic mutation of p53 was sufficient to activate the Aha1/Hsp90 ATPase axis leading to stimulation of Wnt signaling and increased expression of Wnt target genes. PMID: 24451373
  88. Heat shock protein 90 controls HIV-1 reactivation from latency. PMID: 24706778
  89. HSP90 from keratinocytes and mast cells is a key regulator of psoriatic inflammation and HSP90 inhibitors may represent a novel therapeutic approach to the disease. PMID: 24521827
  90. SGK3 stability and kinase activation are regulated by the Hsp90-Cdc37 chaperone complex. PMID: 24379398
  91. The correlation of Her2 expression with the expression of Her2 chaperoning HSP90 may indicate a synergistic regulation. PMID: 23874879
  92. Studies indicate that Hsp90 is a highly ubiquitous protein expressed in all organisms. PMID: 24389346
  93. Our results reveal an upregulated Hsp90 expression at the site of inflammation and an autoantibody-mediated dysregulation of the intracellular and extracellular distribution of this chaperone in BP patients. PMID: 23936217
  94. The frequency of mutant CC genotype for HSP90AA1 (rs4947C/T), mutant AA genotype for HSP90AB1 (rs13296A/G) and mutant CC genotype for HSP90B1 (rs2070908 C/G) was significantly higher in the patient group than in controls. PMID: 24511009
  95. eHsp90 upregulates IL-6 and IL-8, cytokines that play a causative role in prostate cancer progression. eHsp90alpha also activated oncogenic inflammatory mediator STAT3 and induced expression of MMP-3, a mediator of fibrosis and the myofibroblast phenotype. PMID: 24338924
  96. PKCgamma plays a critical role in cancer cells, and simultaneous inhibition of PKCgamma and Hsp90alpha synergistically prevents cell migration and promotes apoptosis in cancer cells. PMID: 24117238
  97. Data suggest heat shock protein 90 (HSP90) could be a promising new target in malignant pheochromocytoma (PHEO). PMID: 23873112
  98. Data show that the inactive conformation of ERBB2 does not interact with HSP90 chaperone. PMID: 23844194
  99. Inhibition of HSP90 activity in HTLV-1-transformed cells elicited proteasomal degradation of Tax in the nuclear matrix and inhibition of NF-kappaB and HTLV-1 long terminal repeat activation. PMID: 24109220
  100. Hsp90alpha utilizes a unique transmembrane signaling mechanism via the NPVY motif of LRP-1 to promote cell motility and wound healing. PMID: 24126057

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Subcellular Location Nucleus, Cytoplasm, Melanosome, Cell membrane
Protein Families Heat shock protein 90 family
Database Links

HGNC: 5253

OMIM: 140571

KEGG: hsa:3320

STRING: 9606.ENSP00000335153

UniGene: Hs.525600

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