Code | CSB-RA284992A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
FC | 1:20-1:200 |
To prepare the IRAK4 recombinant monoclonal antibody, the gene coding for the IRAK4 monoclonal antibody is obtained first. Animals were immunized with a synthesized peptide derived from human IRAK4, and B cells were isolated and fused with myeloma cells to create hybridomas. The hybridomas that produce the IRAK4 antibody were cultured to secret the IRAK4 monoclonal antibody, which was collected and sequenced. The IRAK4 monoclonal antibody gene was cloned into a vector. This recombinant vector was then transfected into cells for cultivation, and the resulting IRAK4 recombinant monoclonal antibody was isolated and purified using affinity chromatography from the cell culture supernatant. This antibody has been tested in ELISA, WB, and FC applications for the recognition of human IRAK4 protein.
The IRAK4 protein plays a crucial role in the immune response by transmitting signals from various Toll-like receptors (TLRs) and IL-1Rs. Upon activation of TLRs or IL-1Rs, IRAK4 interacts with other IRAK family members and downstream adaptors such as MyD88 to activate NF-κB and MAPK pathways, leading to the expression of pro-inflammatory cytokines and chemokines. In addition to its role in innate immunity, IRAK4 is also involved in adaptive immunity by regulating T cell activation and differentiation. Mutations in the IRAK4 gene have been linked to primary immunodeficiency disorders, leading to increased susceptibility to infections.
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