N Recombinant Monoclonal Antibody, Biotin conjugated

Code CSB-RA33255D1GMY
Size US$210
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Product Details

Uniprot No.
Target Names
N (Nucleoprotein)
Alternative Names
N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
Species Reactivity
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)
Immunogen Species
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Mouse scFv fusion with human IgG1 Fc
Clone No.
Purification Method
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Tested Applications
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

The production of the N recombinant monoclonal antibody is carried out through a systematic and thorough process to ensure its quality and specificity. The journey begins by isolating B cells from the spleen of an immunized animal, where the recombinant human SARS-CoV-2 N protein (1-419aa) is utilized as the immunogen. The RNA is then extracted from these B cells and converted into cDNA through reverse transcription. Using specific primers designed for the antibody constant regions, the N antibody genes are amplified and inserted into an expression vector. The human IgG1 Fc is integrated into the vector, downstream of the N antibody gene. The biotin is also inserted into the vector. The recombinant vector is then introduced into host cells through transfection, enabling the production of the N recombinant monoclonal antibody. Following an appropriate incubation period, the antibody is collected from the cell culture supernatant and subjected to purification using affinity chromatography, ensuring a high level of purity. To validate its specificity and functionality in detecting human SARS-CoV-2 N protein, the purified N recombinant monoclonal antibody undergoes rigorous characterization through ELISA. This meticulous production process yields a reliable and effective N recombinant monoclonal antibody, serving as a valuable tool in various research related to SARS-CoV-2.

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Target Background

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
Subcellular Location
Virion. Host cytoplasm.
Protein Families
Betacoronavirus nucleocapsid protein family
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