N Recombinant Monoclonal Antibody

Code CSB-RA33255A2GMY
Size US$210
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  •  The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N
    Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC50 is 2.928 ng/ml.
  • In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A2GMY) is clean, the detection limit can be as low as 15.625ng/ml (1.09ng/0.07ml), and the sensitivity is very good.
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Product Details

Uniprot No.
Alternative Names
N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N
Species Reactivity
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)
Immunogen Species
Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)
Mouse scFv fusion with human IgG1 Fc
Clone No.
Purification Method
It differs from different batches. Please contact us to confirm it.
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Tested Applications
Recommended Dilution
Application Recommended Dilution
ELISA 1:10000-1:50000
GICA 1:500-1:5000
Troubleshooting and FAQs
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

The generation of the N recombinant monoclonal antibody involves a rigorous process to ensure its exceptional quality and specificity. Firstly, B cells are isolated from the spleen of an immunized animal, with the recombinant human SARS-CoV-2 N protein (1-419aa) used as the immunogen. RNA is extracted from the B cells and converted into cDNA through reverse transcription. The N antibody genes are then amplified using specific primers designed for the antibody constant regions and cloned into an expression vector. The human IgG1 Fc is inserted into the vector, downstream of the N antibody. This recombinant vector is transfected into host cells to enable the production of the N recombinant monoclonal antibody. After a period of cell culture, the antibody is collected from the cell culture supernatant and purified using affinity chromatography, resulting in a highly purified form suitable for various applications. To ensure its quality and functionality, the antibody undergoes thorough characterization through assays including ELISA and GICA which validate its specificity and ability to detect human SARS-CoV-2 N protein. The meticulous production process guarantees the development of a reliable and effective N recombinant monoclonal antibody, essential for a wide range of human SARS-CoV-2-related research.

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Target Background

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.
Subcellular Location
Virion. Host cytoplasm.
Protein Families
Betacoronavirus nucleocapsid protein family
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