N Recombinant Monoclonal Antibody

^{Rare Species}

Datasheet

Code	CSB-RA33255A2GMY
Size	US$210
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Image	The Binding Activity of SARS-CoV-2-N Antibody with SARS-CoV-2-N Activity: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV-2-N at 2 μg/ml can bind SARS-CoV-2-N Antibody, the EC₅₀ is 2.928 ng/ml. In the Colloidal Gold Immunochromatography Assay detection system, the background of antibody (CSB-RA33255A2GMY) is clean, the detection limit can be as low as 15.625ng/ml (1.09ng/0.07ml), and the sensitivity is very good.
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Product Details
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Target Background

Product Details

Uniprot No.

P0DTC9

Alternative Names

N; Nucleoprotein; N; Nucleocapsid protein; NC; Protein N

Species Reactivity

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Immunogen

Recombinant Human Novel Coronavirus Nucleoprotein (N) (1-419aa) (CSB-EP3325GMY)

Immunogen Species

Human Novel Coronavirus (SARS-CoV-2/ 2019-nCoV)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Mouse scFv fusion with human IgG1 Fc

Clone No.

4D2

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, GICA

Recommended Dilution

Application	Recommended Dilution
ELISA	1:10000-1:50000
GICA	1:500-1:5000

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The SARS-CoV-2 nucleocapsid protein serves as one of the most abundant viral proteins during infection, playing essential roles in viral RNA packaging, replication, and host cell interactions. Its high immunogenicity and conservation across viral variants have established it as a primary target for COVID-19 diagnostic development and seroprevalence studies, making reliable detection reagents critical for ongoing coronavirus research.

This recombinant monoclonal antibody targeting the SARS-CoV-2 nucleocapsid protein offers researchers the consistency and reproducibility that recombinant technology provides. Generated against the full-length nucleoprotein sequence spanning amino acids 1-419, the antibody is produced as a mouse scFv fusion with human IgG1 Fc, combining the specificity of monoclonal selection with a defined molecular format. Because the antibody sequence is fixed and production occurs in controlled expression systems, researchers can expect uniform performance across different lots, eliminating the variability concerns that can complicate longitudinal studies or assay development workflows.

Validation data demonstrate exceptional binding characteristics, with functional ELISA testing revealing an EC50 of 2.928 ng/ml when binding to immobilized SARS-CoV-2 nucleocapsid protein. For rapid detection applications, colloidal gold immunochromatography assay testing shows clean background signal with detection sensitivity reaching as low as 15.625 ng/ml, supporting its utility in point-of-care or high-throughput screening contexts. The recommended working dilutions span 1:10000 to 1:50000 for ELISA and 1:500 to 1:5000 for immunochromatographic applications, offering flexibility to optimize signal-to-noise ratios across different experimental setups.

This antibody supports researchers developing diagnostic platforms, studying viral protein biology, or investigating host-pathogen interactions in the context of SARS-CoV-2 infection.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Packages the positive strand viral genome RNA into a helical ribonucleocapsid (RNP) and plays a fundamental role during virion assembly through its interactions with the viral genome and membrane protein M. Plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. May modulate transforming growth factor-beta signaling by binding host smad3.

Subcellular Location

Virion. Host cytoplasm.

Protein Families

Betacoronavirus nucleocapsid protein family

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Virus Infection Target Therapy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

No. 269, Shendun 5th Road, Donghu Hi-Tech Development Area, Hubei Province, 430206, P.R.China

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