Code | CSB-RA121182A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
IHC | 1:20-1:200 |
The NCAM recombinant monoclonal antibody expression typically involves the initial step of inserting the NCAM antibody-encoding gene into expression vectors. These vectors are then delivered into host cells via polyethyleneimine-mediated transfection. Culturing of the host cells leads to the production and secretion of the antibodies. Post-affinity chromatography purification, the antibodies' functionality is assessed through ELISA, WB, and IHC tests, confirming their capacity to recognize the human NCAM protein.
NCAM is a versatile cell adhesion molecule that plays a central role in various aspects of neural development, structure, and function. Its functions extend to neuronal migration, axon guidance, synaptic plasticity, cellular differentiation, and the response to neural injury.
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