PKN2 Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA921617A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, U251 whole cell lysate, PC3 whole cell lysate, MCF-7 whole cell lysate All lanes: PKN2 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 113, 117, 112 kDa Observed band size: 130 kDa IHC image of CSB-RA921617A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of MCF-7 cell with CSB-RA921617A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 509-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Overlay Peak curve showing MCF7 cells stained with CSB-RA921617A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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Product Details
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Target Background

Product Details

Uniprot No.

Q16513

Target Names

PKN2

Alternative Names

Serine/threonine-protein kinase N2 (EC 2.7.11.13) (PKN gamma) (Protein kinase C-like 2) (Protein-kinase C-related kinase 2), PKN2, PRK2 PRKCL2

Species Reactivity

Human

Immunogen

A synthesized peptide derived from human PKN2

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

20A8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:50-1:200
IF	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The PKN2 recombinant monoclonal antibody is produced through a well-established and rigorous process to ensure its quality and specificity. Initially, B cells are isolated from an immunized animal using the synthesized peptide derived from human PKN2 as the immunogen. Total RNA is extracted from the B cells and converted into cDNA through reverse transcription. The PKN2 antibody genes are then amplified using specific primers designed for the antibody constant regions and inserted into an expression vector. This vector is transfected into host cells, enabling the production of the PKN2 recombinant monoclonal antibody. Following cell culture, the antibody is harvested from the supernatant and purified using affinity chromatography, resulting in a highly purified preparation. Extensive characterization assays, including ELISA, WB, IHC, IF, and FC analysis, are conducted to validate the antibody's specificity and functionality, ensuring its precise recognition of human PKN2 protein. This meticulous production process guarantees the generation of a reliable and effective PKN2 recombinant monoclonal antibody, suitable for diverse applications in PKN2-related research.

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Target Background

Function

PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Involved in the negative regulation of ciliogenesis.; (Microbial infection) Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication.

Gene References into Functions

The expression of PKN2 in colon cancer cells suppresses tumor associated M2 macrophage polarization and tumor growth. PMID: 29368606
PKN2 knockdown in vitro decreased insulin-stimulated glucose uptake, incorporation into glycogen, and oxidation. Thus, PKN2 alters key signaling pathways and transcriptional networks to regulate glucose and lipid metabolism. PMID: 28720584
Helicobacter pylori CagA interacts with PRK2 and inhibits its kinase activity. PMID: 26041307
findings demonstrate that Yersinia enterocolitica rYopM interacts with RSK1 and PRK2 following cell-penetration PMID: 25513777
Regulation of protein kinase C-related protein kinase 2 (PRK2) by an intermolecular PRK2-PRK2 interaction mediated by Its N-terminal domain. PMID: 22511787
these findings suggest that Hsp90 plays a critical role in the regulation of HCV RNA polymerase phosphorylation via the PDK1-PRK2 signaling pathway. PMID: 22490666
Rho binding is essential for PRK2 function and facilitates PRK2 recruitment to junctions. Kinase-dead PRK2 acts as a dominant-negative mutant and prevents apical junction formation. PMID: 20974804
PRK2/PKN2, is an essential regulator of both entry into mitosis and exit from cytokinesis in HeLa S3 cells, required for abscission of the midbody at the end of the cell division cycle and for phosphorylation and activation of Cdc25B. PMID: 17332740
These observations highlight elements of Nef's functional complexity and demonstrate previously unsuspected structural requirements for PAK-2 activation and MHC-1 down-modulation in Nef's flexible N- and C-terminal regions. PMID: 17632197
Little evidence of association was observed between SNPs in PKN2 and type 2 diabetes in African Americans. PMID: 18443202
the last seven amino acid residues at the C-terminus of PRK2 are not required for the activation of the kinase by RhoA in vitro, however, the extreme C-terminal segment is critical for the full activation of PRK2 by RhoA in cells PMID: 18835241

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Subcellular Location

Cytoplasm. Nucleus. Membrane. Cell projection, lamellipodium. Cytoplasm, cytoskeleton. Cleavage furrow. Midbody. Cell junction.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily

Tissue Specificity

Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells.

Database Links

HGNC: 9406

OMIM: 602549

KEGG: hsa:5586

STRING: 9606.ENSP00000359552

UniGene: Hs.440833

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

PI3K-Akt signaling pathway

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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