Code | CSB-RA056240A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
FC | 1:50-1:200 |
To produce the PKN2 recombinant monoclonal antibody, in vitro expression systems are harnessed, involving the cloning of DNA sequences of PKN2 antibodies obtained from immunoreactive rabbits. The immunogen used is a synthesized peptide derived from the human PKN2 protein. Subsequently, the genes encoding the PKN2 antibodies are inserted into plasmid vectors, and these recombinant plasmid vectors are transfected into host cells to enable antibody expression. The PKN2 recombinant monoclonal antibody then undergoes affinity-chromatography purification and is extensively tested for functionality in ELISA, WB, and FC applications, confirming its reactivity with the human PKN2 protein.
PKN2 is a multifunctional protein kinase involved in a wide range of cellular processes, including cytoskeletal organization, cell migration, cell proliferation, and signaling pathways. Its diverse roles make it a crucial player in normal cellular physiology and contribute to its relevance in cancer biology and other diseases.
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