Product Details

Purity

>85% (SDS-PAGE)

Target Names

PKN2

Uniprot No.

Q16513

Alternative Names

Cardiolipin activated protein kinase Pak2; MGC150606; MGC71074; PAK 2; PAK2; PKN gamma; PKN2; PKN2_HUMAN; PRK2; PRKCL2; PRO2042; Protein kinase C like 2; Protein kinase C related kinase 2; Protein kinase C-like 2; Protein kinase N2 ; Protein-kinase C-related kinase 2; Serine/threonine-protein kinase N2

Species

Homo sapiens (Human)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Involved in the negative regulation of ciliogenesis.; (Microbial infection) Phosphorylates HCV NS5B leading to stimulation of HCV RNA replication.

Gene References into Functions

The expression of PKN2 in colon cancer cells suppresses tumor associated M2 macrophage polarization and tumor growth. PMID: 29368606
PKN2 knockdown in vitro decreased insulin-stimulated glucose uptake, incorporation into glycogen, and oxidation. Thus, PKN2 alters key signaling pathways and transcriptional networks to regulate glucose and lipid metabolism. PMID: 28720584
Helicobacter pylori CagA interacts with PRK2 and inhibits its kinase activity. PMID: 26041307
findings demonstrate that Yersinia enterocolitica rYopM interacts with RSK1 and PRK2 following cell-penetration PMID: 25513777
Regulation of protein kinase C-related protein kinase 2 (PRK2) by an intermolecular PRK2-PRK2 interaction mediated by Its N-terminal domain. PMID: 22511787
these findings suggest that Hsp90 plays a critical role in the regulation of HCV RNA polymerase phosphorylation via the PDK1-PRK2 signaling pathway. PMID: 22490666
Rho binding is essential for PRK2 function and facilitates PRK2 recruitment to junctions. Kinase-dead PRK2 acts as a dominant-negative mutant and prevents apical junction formation. PMID: 20974804
PRK2/PKN2, is an essential regulator of both entry into mitosis and exit from cytokinesis in HeLa S3 cells, required for abscission of the midbody at the end of the cell division cycle and for phosphorylation and activation of Cdc25B. PMID: 17332740
These observations highlight elements of Nef's functional complexity and demonstrate previously unsuspected structural requirements for PAK-2 activation and MHC-1 down-modulation in Nef's flexible N- and C-terminal regions. PMID: 17632197
Little evidence of association was observed between SNPs in PKN2 and type 2 diabetes in African Americans. PMID: 18443202
the last seven amino acid residues at the C-terminus of PRK2 are not required for the activation of the kinase by RhoA in vitro, however, the extreme C-terminal segment is critical for the full activation of PRK2 by RhoA in cells PMID: 18835241

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Subcellular Location

Cytoplasm. Nucleus. Membrane. Cell projection, lamellipodium. Cytoplasm, cytoskeleton. Cleavage furrow. Midbody. Cell junction.

Protein Families

Protein kinase superfamily, AGC Ser/Thr protein kinase family, PKC subfamily

Tissue Specificity

Ubiquitous. Expressed in numerous tumor cell lines, especially in bladder tumor cells.

Database Links

HGNC: 9406

OMIM: 602549

KEGG: hsa:5586

STRING: 9606.ENSP00000359552

UniGene: Hs.440833

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Recombinant Human Serine/threonine-protein kinase N2 (PKN2), partial

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