Phospho-BRAF (T401) Antibody

Datasheet
Code CSB-RA002791A401phHU
Size US$350
Uniprot No. P15056
Abbreviation Serine/threonine-protein kinase B-raf
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image
  • Western Blot
    Positive WB detected in:A549 whole cell lysate(treated with EGF or not)
    All lanes:Phospho-BRAF antibody at 1.3ug/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 85 KDa
    Observed band size: 85 KDa

  • Immunofluorescence staining of HepG2 cells with CSB-RA002791A401phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunogen A synthesized peptide derived from human Phospho-BRAF (T401)
Species Reactivity Human
Tested Applications ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:20-1:200
Relevance Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purification Method Affinity-chromatography
Isotype Rabbit IgG
Clonality Monoclonal
Alias Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1, BRAF, BRAF1, RAFB1
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Research Area Cell Biology
Gene Names BRAF
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Function Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Involvement in disease Note=Defects in BRAF are found in a wide range of cancers. {ECO:0000269|PubMed:18974108}.; Colorectal cancer (CRC)
Subcellular location Nucleus, Cytoplasm, Cell membrane
Pathway cAMP signaling pathway
Chemokine signaling pathway
ErbB signaling pathway
MAPK signaling pathway
mTOR signaling pathway
Focal adhesion
Regulation of actin cytoskeleton
Vascular smooth muscle contraction
MAPK Erk pathway
Natural killer cell mediated cytotoxicity
FoxO signaling pathway
Insulin signaling pathway
Rap1 signaling pathway
Neurotrophin signaling pathway

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