Code | CSB-RA171021A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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IHC | 1:50-1:200 |
B lymphocytes were obtained from an animal immunized with a synthesized peptide derived from human BRAF. These cells were then fused with myeloma cells to generate hybridomas, whose variable light (VL) and variable heavy (VH) domains were sequenced to create a vector for recombinant generation. The resulting vector was transfected into cells for culture and production of the BRAF recombinant monoclonal antibody. The antibody was purified using affinity chromatography, and its specificity for human BRAF was verified using ELISA and IHC applications.
The BRAF protein is a member of the Raf family of serine/threonine kinases and plays an important role in the regulation of cell growth and proliferation. When activated, BRAF can phosphorylate and activate the downstream effector MEK1/2, which in turn activates the MAPK/ERK signaling pathway. This pathway is involved in many cellular processes, including cell proliferation, differentiation, survival, and apoptosis. Mutations in BRAF are commonly found in a variety of cancers, including melanoma, colorectal cancer, and thyroid cancer.
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