Code | CSB-RA023984A1HU |
Size | US$210 |
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The generation of the TNFRSF9/4-1BB recombinant monoclonal antibody involves a meticulous and controlled process to ensure its exceptional quality and specificity. It begins by isolating B cells from the spleen of an immunized animal, where the recombinant human TNFRSF9 protein is used as the immunogen. The extracted RNA from the B cells is converted into cDNA through reverse transcription. The TNFRSF9 antibody genes are then amplified using specific primers designed for the antibody constant regions and inserted into an expression vector. This vector is subsequently transfected into host cells, allowing for the production of the TNFRSF9/4-1BB recombinant monoclonal antibody. After a period of cell culture, the antibody is harvested from the supernatant and purified using affinity chromatography, resulting in a highly purified form suitable for various applications. ELISA is conducted to validate the antibody's specificity and functionality in detecting human TNFRSF9 protein. This stringent production process ensures the generation of a reliable and effective TNFRSF9 recombinant monoclonal antibody, essential for diverse TNFRSF9-related research and diagnostic applications.
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