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Rabbit anti-Mouse IgG Fc fragment polyclonal Antibody;HRP conjugated
Product Name Rabbit anti-Mouse IgG Fc fragment polyclonal Antibody;HRP conjugated
Synonyms
Catalog Number CSB-PA00580F0Rb
Immunogen Mouse IgG Fc fragment
Raised in Rabbit
Species Reactivity Mouse
Spectificity
Tested applications WB;ELISA
Application notes
Relevance The fragment crystallizable region (Fc region) is the tail region of an antibody that interacts with cell surface receptors called Fc receptors and some proteins of the complement system. This property allows antibodies to activate the immune system. InIgG, IgA and IgD antibody isotypes, the Fc region is composed of two identical protein fragments, derived from the second and third constant domains of the antibodys two heavy chains; IgM and IgE Fc regions contain three heavy chain constant domains (CH domains 2C4) in each polypeptide chain. The Fc regions of IgGs bear a highly conserved N-glycosylation site. Glycosylation of the Fc fragment is essential for Fc receptor-mediated activity. The N-glycans attached to this site are predominantly core-fucosylated diantennary structures of the complex type. In addition, small amounts of these N-glycans also bear bisecting GlcNAc and -2,6 linked sialic acid residues.
The other part of an antibody, called the Fab region, contains variable sections that define the specific target that the antibody can bind. By contrast, the Fc region of all antibodies in a class are the same for each species; they are constant rather than variable. The Fc region is, therefore, sometimes incorrectly termed the "fragment constant region".
Clonality Polyclonal
Clone Number
Isotype IgG
Purity Caprylic Acid Ammonium Sulfate Precipitation purified
Conjugate HRP conjugated
Stroage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Stroage Shipped at 4C. Upon delivery aliquot and store at -20C or -80C. Avoid repeated freeze.
References
Images


Western blot





All lanes : Mouse IgG Fc fragment antibody at 2 µg/ml +mouse serum

 

Lane 1 : mouse serum at 1 : 100

Lane 2 : mouse serum at 1 : 1000


 

Secondary
Goat polyclonal to Rabbit IgG at 1/15000 dilution
 

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