Bovine Interleukin 6,IL-6 ELISA Kit

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Bovine Interleukin 6,IL-6 ELISA Kit

Name: Bovine Interleukin 6,IL-6 ELISA Kit
Brand: CUSABIO
SKU: CSB-E12899B

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Code	CSB-E12899B
Size	96T,5×96T,10×96T
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Target Data

Target Name

interleukin 6 (interferon, beta 2)

Alternative Names

IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit

Abbreviation

IL6

Uniprot No.

P26892

Species

Bos taurus (Bovine)

Sample Types

serum, plasma, tissue homogenates

Detection Range

5 pg/mL-1000 pg/mL

Sensitivity

2.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-97
1:2	Average %	95
	Range %	91-99
1:4	Average %	95
	Range %	88-101
1:8	Average %	95
	Range %	90-100

Recovery

The recovery of bovine IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	94-101
EDTA plasma (n=4)	96	92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.071	0.075	0.073
250	0.192	0.205	0.199
80	0.421	0.451	0.436
20	0.876	0.897	0.887
5	1.345	1.316	1.331
0	2.116	2.215	2.166

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Haemato-chemical and immune variations in Holstein cows at different stages of lactation, parity, and age SB Kim,CAAS Agricultural Journals,2020
Enhancing dietary cation-anion difference reshaped the distribution of endotoxin across different biofluids and influenced inflammatory response in dairy cows … Z.Wang,Animal Feed Science and Technology,2020
Protective Effects of Astaxanthin on Lipopolysaccharide-Induced Inflammation in Bovine Endometrial Epithelial Cells Wan F C, et al,Biology of Reproduction,2019
Caffeic Acid Prevented LPS-Induced Injury of Primary Bovine Mammary Epithelial Cells through Inhibiting NF-κB and MAPK Activation Mingjiang Liu,Mediators of Inflammation,2019
Supplementation with fat-coated rumen-protected glucose during the transition period enhances milk production and influences blood biochemical parameters of liver function and inflammation in dairy cows X.P.Li,Animal Feed Science and Technology,2019
Immune Responses of Cattle to Theileria orientalis Infection and Seasonal Change Changyong Choe, et al,International Journal of Applied Research in Veterinary Medicine,2018
Serum hepatokines in dairy cows: periparturient variation and changes in energy-related metabolic disorders Jianguo Wang.et al,BMC Veterinary Research,2018
Milk fever in dairy cows is preceded by activation of innate immunity and alterations in carbohydrate metabolism prior to disease occurrence Zhang G.et al,Res Vet Sci,2017
Effects of continuous low dose infusion of lipopolysaccharide on inflammatory responses, milk production and milk quality in dairy cows Ning LT.et al,J Anim Physiol Anim Nutr ,2017
Kinetics of selected plasma cytokines during innate-adaptive immune response transition in adult cattle infected with the bovine ephemeral fever virus R. Barigye.et al,Veterinary Microbiology,2016
Detection of serum amyloid-A concentration in the calf clinically diagnosed with pneumonia, enteritis and pneumoenteritis Mustafa Kabu. et al,/,2016
Concentrations of serum amyloid A, haptoglobin, tumour necrosis factor and interleukin-1 and -6 in Anatolian buffaloes naturally infected with dermatophytosis M. Kabu.et al,Veterinarni Medicina,2016
Concentrations of serum amyloid A, haptoglobin,tumour necrosis factor and interleukin-1 and -6 in Anatolian buffaloes naturally infected with dermatophytosis Kabu M,.et al,Veterinarni Medicina,2016
Acute-phase responses in cattle infected with hydatid cysts and microbial agents. Sevimli A.et al,J Helminthol.,2015
Kinetics of pro-inflammatory cytokines, interleukin-10, and virus neutralising antibodies during acute ephemeral fever virus infections in Brahman cattle Barigye R. et al,Vet Immunol Immunopathol,2015
A small scale study on the effects of oral administration of the ß-glucan produced by Aureobasidium pullulans on milk quality and cytokine expressions of Holstein cows, and on bacterial flora in the intestines of Japanese black calves Hirofumi Uchiyama et al,BMC Research Notes,2012
Contrasting Cellularity and Fatty Acid Composition in Fat Depots from Alentejana and Barrosã Bovine Breeds Fed High and Low Forage Diets /,Int J Biol Sci,2012

Function	Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity).
Gene References into Functions	STA3 facilitates TLR4-dependent IL-6 and IL-8 production via IL-6 receptor-positive feedback in endometrial cells. PMID: 26813342 when a confluent endometrial epithelial cell barrier is faced with infection and damage, chemokines attract immune cells to the uterine lumen, but IL6 is solely secreted apically to ensure immune cells are only exposed to IL6 once they reach the lumen. PMID: 25740541 The results revealed that the peak expression of IL6 and 21 was on DPV 28 which correlated well with the FMDV antibody titer and plummeted to the prevaccination titer level by 60 DPV. PMID: 24555796 Exposure to follicular fluid transiently increased the transcript levels of IL8 and PTGS2, and decreased the expression of SOD2, GPX3, DAB2, and NR3C1. TNF and IL6 levels were also decreased while those of NAMPT were unaffected. PMID: 24186266 Testicular IL-1 alpha and IL-1 beta concentrations were highest in the early post-natal period; however, IL-1 bioactivity and IL-6 concentrations were greatest in the immediate pre-pubertal period. PMID: 19144032 The results showed that the expression of TNF-alpha, iNOS, and IL-6 in alveolar macrophages was up-regulated by stimulation with the recombinant Mce4A protein of M. bovis; in contrast, expression of IL-12 was unaffected.[IL-6, IL-12] PMID: 17530193 results show for the first time that interleukin-6 (IL6), in the presence of its soluble receptor (sIL-6R), induces activation of JAK1, JAK2, and STAT1/STAT3 proteins in bovine articular chondrocytes. PMID: 12419823 Mechanical injury potentiates the catabolic effects of TNFalpha and IL-6/sIL-6R in causing proteoglycan degradation in human and bovine cartilage. PMID: 19790045 Show More Hide All
Subcellular Location	Secreted
Protein Families	IL-6 superfamily
Database Links	KEGG: bta:280826 STRING: 9913.ENSBTAP00000019865 UniGene: Bt.3686

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

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