Bovine Interleukin 6,IL-6 ELISA Kit

Bovine Interleukin 6,IL-6 ELISA Kit

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IL6 Proteins

IL6 Proteins

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Bovine Interleukin 6,IL-6 ELISA Kit

Specification
Target

Code

CSB-E12899B

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Product Type

ELISA Kit

Size

96T,5×96T,10×96T

Uniprot No.

P26892

Lead Time

3-5 working days

Abbreviation

IL6

Protein Biological Process 1

Immunity

Target Name

interleukin 6 (interferon, beta 2)

Alias

Species

Bos taurus (Bovine)

Protein Biological Process 3

Acute phase

Sample Types

serum, plasma, tissue homogenates

Detection Range

5 pg/mL-1000 pg/mL

Sensitivity

2.5 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Protocol

Instructions

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.

Assay Principle

quantitative

Measurement

Competitive

Target Details

This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis.

HGNC

1834

RGD

2327

MGI

88373

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	90-97
1:2	Average %	95
	Range %	91-99
1:4	Average %	95
	Range %	88-101
1:8	Average %	95
	Range %	90-100

Recovery

The recovery of bovine IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	94-101
EDTA plasma (n=4)	96	92-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.071	0.075	0.073
250	0.192	0.205	0.199
80	0.421	0.451	0.436
20	0.876	0.897	0.887
5	1.345	1.316	1.331
0	2.116	2.215	2.166

References

Contrasting Cellularity and Fatty Acid Composition in Fat Depots from Alentejana and Barrosã Bovine Breeds Fed High and Low Forage Diets /,Int J Biol Sci,2012 ;8(2):214-27.
A small scale study on the effects of oral administration of the ß-glucan produced by Aureobasidium pullulans on milk quality and cytokine expressions of Holstein cows, and on bacterial flora in the intestines of Japanese black calves Hirofumi Uchiyama et al,BMC Research Notes,2012 Jun 19;5:189.
Kinetics of pro-inflammatory cytokines, interleukin-10, and virus neutralising antibodies during acute ephemeral fever virus infections in Brahman cattle Barigye R. et al,Vet Immunol Immunopathol,Sep 12.pii: S0165-2427(15)00201-9.
Acute-phase responses in cattle infected with hydatid cysts and microbial agents. Sevimli A.et al,J Helminthol.,89(4):471-9
Concentrations of serum amyloid A, haptoglobin,tumour necrosis factor and interleukin-1 and -6 in Anatolian buffaloes naturally infected with dermatophytosis Kabu M,.et al,Veterinarni Medicina,61(3): 133-135.
Concentrations of serum amyloid A, haptoglobin, tumour necrosis factor and interleukin-1 and -6 in Anatolian buffaloes naturally infected with dermatophytosis M. Kabu.et al,Veterinarni Medicina,2016 (3):133-135
Detection of serum amyloid-A concentration in the calf clinically diagnosed with pneumonia, enteritis and pneumoenteritis Mustafa Kabu. et al,/,2016 46(2): 293-299
Kinetics of selected plasma cytokines during innate-adaptive immune response transition in adult cattle infected with the bovine ephemeral fever virus R. Barigye.et al,Veterinary Microbiology,/
Effects of continuous low dose infusion of lipopolysaccharide on inflammatory responses, milk production and milk quality in dairy cows Ning LT.et al,J Anim Physiol Anim Nutr ,May 15
Milk fever in dairy cows is preceded by activation of innate immunity and alterations in carbohydrate metabolism prior to disease occurrence Zhang G.et al,Res Vet Sci,Dec 19
Serum hepatokines in dairy cows: periparturient variation and changes in energy-related metabolic disorders Jianguo Wang.et al,BMC Veterinary Research,August 13
Immune Responses of Cattle to Theileria orientalis Infection and Seasonal Change Changyong Choe, et al,International Journal of Applied Research in Veterinary Medicine,/

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Function	Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Required for the generation of T(H)17 cells. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation (By similarity).
Subcellular Location	Secreted
Protein Families	IL-6 superfamily
Database Links	KEGG: bta:280826 STRING: 9913.ENSBTAP00000019865 UniGene: Bt.3686
Pathway	HIF-1 signaling pathway Jak-STAT signaling pathway PI3K-Akt signaling pathway TNF signaling pathway Cellular senescence Hematopoietic cell lineage IL-17 signaling pathway NOD-like receptor signaling pathway Th17 cell differentiation Toll-like receptor signaling pathway AGE-RAGE signaling pathway in diabetic complications FoxO signaling pathway Inflammatory Pain

IL6 Protein

IL6 Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP011664BO CSB-EP011664BO CSB-BP011664BO CSB-MP011664BO	Recombinant Bovine Interleukin-6(IL6)	Yeast E.coli Baculovirus Mammalian cell

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.