Fish Tumor Necrosis Factor α(TNF-α) ELISA KIT

Product Details

Citations

Customer Reviews and Q&A

Target Background

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Fish Tumor Necrosis Factor α(TNF-α) ELISA KIT

Name: Fish Tumor Necrosis Factor α(TNF-α) ELISA KIT
Brand: CUSABIO
SKU: CSB-E13254Fh
Price: 150 USD
Availability: InStock
Rating: 5.0 (2 reviews)

Citations (14) Protocol

Code	CSB-E13254Fh
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (14)
Customer Reviews and Q&A
Target Background

Abbreviation

TNF-α

Uniprot No.

Q8JFG3

Species

Fish

Sample Types

serum, plasma

Detection Range

125 pg/mL-5000 pg/mL

Sensitivity

125 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Competitive

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of fish TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	89-100
1:2	Average %	93
	Range %	87-99
1:4	Average %	95
	Range %	88-103
1:8	Average %	96
	Range %	90-102

Recovery

The recovery of fish TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-102
EDTA plasma (n=4)	95	91-101

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
5000	0.154	0.156	0.155
2500	0.351	0.328	0.340
1150	0.562	0.535	0.549
375	0.861	0.864	0.863
125	1.544	1.530	1.537
0	2.387	2.355	2.371

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Fish TNF-α ELISA Kit was designed for the quantitative measurement of Fish TNF-α protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 125 pg/mL-5000 pg/mL and the sensitivity is 125 pg/mL.

Integrative Assessment of Ficus septica as a Functional Phytogenic Additive: Nutritional, Antioxidant, and Immunomodulatory Efficacy in Betta picta Challenged with Aeromonas hydrophila RA Islamy, M Czech, AS Kamarudin, J South,Nongye Jixie Xuebao/Transactions of the Chinese Society of Agricultural Machinery,2025
Gut dysbiosis mediates neurotoxic effects of environmentally relevant tylosin exposure in adult zebrafish W Li, N Wang, D Lyu, X Yu, X He, K Yu, Y Qiu,Ecotoxicology and Environmental Safety,2025
Mitigation of benzo(a)pyrene-induced hepatotoxicity and oxidative damage in Nile tilapia (Oreochromis niloticus) using N-acetylcysteine-loaded starch nanoparticles in porous alginate beads through the CYP1A/Nrf2/HO-1 pathway M Abomosallam, A Ateya, AM Shosha, R Refaat,Aquaculture International,2025
TNF-Α, IL-6, HSP-70, fish growth hormone, and growth performance of sea trout, Salmo trutta (Actinopterygii: Salmoniformes: Salmonidae) after long-term dietary administration of β-glucan and BGN-2 O Revina,/,2023
Irisin inhibits CCK-8-induced TNF-α production via integrin αVβ5-NF-κB signaling pathways in Nile tilapia (Oreochromis niloticus) W Deng,Fish & shellfish immunology,2023
Pectin Nanoparticle-Loaded Soft Coral Nephthea sp. Extract as In Situ Gel Enhances Chronic Wound Healing: In Vitro, In Vivo, and In Silico Studies NH Hassan,Pharmaceuticals,2023
Molecular characterization and immune functions of lipasin in Nile tilapia (Oreochromis niloticus): Involvement in the regulation of tumor necrosis factor-α secretion R Dong,Fish & shellfish immunology,2023
Transcriptome analysis of immune-related gene expression in Yellow River carp (Cyprinus carpio var.) after challenge with Flavobacterium columnare Y Jia,Microbial pathogenesis,2021
Dietary taurine modulates hepatic oxidative status, ER stress and inflammation in juvenile turbot (Scophthalmus maximus L.) fed high carbohydrate diets Yue Zhang,Fish & shellfish immunology,2020
Inclusion of condensed tannins in Lateolabrax japonicus diets: Effects on growth, nutrient digestibility, antioxidant and immune capacity and copper sulphate stress resistance K Peng,Aquaculture Reports,2020
Quercetin affects shoaling and anxiety behaviors in zebrafish: Involvement of neuroinflammation and neuron apoptosis J Zhang,Fish & Shellfish Immunology,2020
Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus Kangyu Deng.et al,SCI REP-UK,2018
Cytokine Responses in Gills of Capoeta umbla as Biomarkers of Environmental Pollution Danabas D.et al,Water Environ Res.,2016
Immunomodulation of Lactobacillus pentosus PL11 against Edwardsiella tarda infection in the head kidney cells of the Japanese eel (Anguilla japonica). Birhanu BT.et al,Fish Shellfish Immunol.,2016

■ Customer Reviews

Average Rating:

5.0 - 2 reviews

Submit a Review here

Sample type: Serum Tissue homogenate Liver

Sample species: Fish

Sample dilution: 1:50

Review: I used CSB-E13254Fh to detect serum and liver homogenate from the normal group. The standard OD values are 2.157, 1.402, 0.928, 0.612, 0.372, 0.186, and the sample OD values are between 0.2 and 1.0.

By Anonymous

Sample type: Serum

Sample species: Fish

Sample dilution: No dilution

Review: I used CSB-E13254Fh to detect the normal group and inflammation group of Micropterus salmoides. The OD values of the standard curve were 2.217/1.409/0.881/0.692/0.414/0.214, and the r value of the standard curve was greater than 0.99, which was relatively accurate.

By Anonymous

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

Function
(From Uniprot)

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.

Subcellular Location

Membrane; Single-pass type II membrane protein.

Protein Families

Tumor necrosis factor family

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Related pathways

MAPK signaling pathway mTOR signaling pathway TGF-beta signaling pathway NF-kappa B signaling pathway TNF signaling pathway Apoptosis Necroptosis Antigen processing and presentation Fc epsilon RI signaling pathway Hematopoietic cell lineage IL-17 signaling pathway Natural killer cell mediated cytotoxicity NOD-like receptor signaling pathway Osteoclast differentiation T cell receptor signaling pathway Toll-like receptor signaling pathway Adipocytokine signaling pathway AGE-RAGE signaling pathway in diabetic complications Inflammatory Pain

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