Human Epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) ELISA Kit

Code CSB-E08178h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P42830
Lead Time 3-5 working days
Abbreviation CXCL5
Protein Biological Process 1 Cytokine
Target Name chemokine (C-X-C motif) ligand 5
Alias ENA-78, SCYB5, epithelial-derived neutrophil activating protein 78|epithelial-derived neutrophil-activating peptide 78|neutrophil-activating peptide ENA-78|neutrophil-activating protein 78|small ind
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.567 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is an inflammatory chemokine that belongs to the CXC chemokine family. This chemokine is produced concomitantly with interleukin-8 (IL8) in response to stimulation with either interleukin-1 (IL1) or tumor necrosis factor-alpha (TNFA). This chemokine is a potent chemotaxin involved in neutrophil activation.
HGNC 10642
RGD 708540
MGI 1096868
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ENA-78/CXCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 98    
Range % 94-103    
1:2 Average % 102    
Range % 97-105    
1:4 Average % 86    
Range % 83-89    
1:8 Average % 97    
Range % 94-100    
Recovery
The recovery of human ENA-78/CXCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 85 81-89    
EDTA plasma (n=4) 98 94-103    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
ng/ml OD1 OD2 Average Corrected    
40 2.282 2.204 2.243 2.131    
20 1.362 1.368 1.365 1.253    
10 0.781 0.757 0.769 0.657    
5 0.435 0.445 0.440 0.328    
2.5 0.304 0.316 0.310 0.198    
1.25 0.214 0.205 0.210 0.098    
0.625 0.188 0.184 0.186 0.074    
0 0.114 0.110 0.112      
References
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Function Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.
Subcellular Location Secreted
Protein Families Intercrine alpha (chemokine CxC) family
Database Links

HGNC: 10642

OMIM: 600324

KEGG: hsa:6374

STRING: 9606.ENSP00000296027

UniGene: Hs.89714

Pathway Chemokine signaling pathway
TNF signaling pathway
IL-17 signaling pathway

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