Name: Human Epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) ELISA Kit
Brand: CUSABIO
SKU: CSB-E08178h

Product Details

Target Name

chemokine (C-X-C motif) ligand 5

Alternative Names

AMCFII ELISA Kit; C-X-C motif chemokine 5 ELISA Kit; C-X-C motif chemokine ligand 5 ELISA Kit; Chemokine (C X C motif) ligand 5 ELISA Kit; chemokine (C-X-C motif) ligand 5 ELISA Kit; Cxcl5 ELISA Kit; CXCL5_HUMAN ELISA Kit; ENA 78 ELISA Kit; ENA-78 (8-78) ELISA Kit; ENA-78(1-78) ELISA Kit; ENA-78(9-78) ELISA Kit; ENA78 ELISA Kit; Epithelial derived neutrophil activating protein 78 ELISA Kit; Epithelial-derived neutrophil-activating protein 78 ELISA Kit; Lipopolysaccharide-induced CXC chemokine ELISA Kit; Neutrophil activating peptide ENA 78 ELISA Kit; Neutrophil activating protein 78 ELISA Kit; Neutrophil-activating peptide ENA-78 ELISA Kit; neutrophil-activating protein 78 ELISA Kit; SCYB5 ELISA Kit; Small inducible cytokine B5 ELISA Kit; small inducible cytokine subfamily B (Cys-X-Cys); member 5 (epithelial-derived neutrophil-activating peptide 78) ELISA Kit; small inducible cytokine subfamily B; member 5 ELISA Kit; Small-inducible cytokine B5 ELISA Kit

Abbreviation

CXCL5

Uniprot No.

P42830

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

0.625 ng/mL-40 ng/mL

Sensitivity

0.567 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ENA-78/CXCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-103
1:2	Average %	102
	Range %	97-105
1:4	Average %	86
	Range %	83-89
1:8	Average %	97
	Range %	94-100

Recovery

The recovery of human ENA-78/CXCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-89
EDTA plasma (n=4)	98	94-103

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
40	2.282	2.204	2.243	2.131
20	1.362	1.368	1.365	1.253
10	0.781	0.757	0.769	0.657
5	0.435	0.445	0.440	0.328
2.5	0.304	0.316	0.310	0.198
1.25	0.214	0.205	0.210	0.098
0.625	0.188	0.184	0.186	0.074
0	0.114	0.110	0.112

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

The product CSB-E08178h is a sandwich ELISA kit developed to measure levels of human ENA-78 in multiple samples, including serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ENA-78 in samples. The color intensity is measured at 450 nm via a microplate reader.

ENA-78, alternatively referred to as CXCL5, is identified as an inflammatory mediator with a powerful role in neutrophil chemotaxis. CXCL5 is upregulated in many cancers including gastric cancer, hepatocellular carcinoma (HCC), breast cancer, and bladder cancer, and is involved in tumor growth and metastasis. In the tumor microenvironment (TME), CXCL5 binds to CXCR2 and participates in the recruitment of immune cells, and promotes angiogenesis, tumor growth, and metastasis. The CXCL5/CXCR2 axis can act as a bridge between tumor cells and host cells in TME. Blocking the transmission of CXCL5/CXCR2 signals can increase the sensitivity and effectiveness of immunotherapy and slow down tumor progression.

Code	CSB-E08178h
Size	$375
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Human Epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) ELISA Kit

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