Human Epithelial neutrophil activating peptide 78 (ENA-78/CXCL5) ELISA Kit

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

Product Details

Alternative Names

AMCFII ELISA Kit; C-X-C motif chemokine 5 ELISA Kit; C-X-C motif chemokine ligand 5 ELISA Kit; Chemokine (C X C motif) ligand 5 ELISA Kit; chemokine (C-X-C motif) ligand 5 ELISA Kit; Cxcl5 ELISA Kit; CXCL5_HUMAN ELISA Kit; ENA 78 ELISA Kit; ENA-78 (8-78) ELISA Kit; ENA-78(1-78) ELISA Kit; ENA-78(9-78) ELISA Kit; ENA78 ELISA Kit; Epithelial derived neutrophil activating protein 78 ELISA Kit; Epithelial-derived neutrophil-activating protein 78 ELISA Kit; Lipopolysaccharide-induced CXC chemokine ELISA Kit; Neutrophil activating peptide ENA 78 ELISA Kit; Neutrophil activating protein 78 ELISA Kit; Neutrophil-activating peptide ENA-78 ELISA Kit; neutrophil-activating protein 78 ELISA Kit; SCYB5 ELISA Kit; Small inducible cytokine B5 ELISA Kit; small inducible cytokine subfamily B (Cys-X-Cys); member 5 (epithelial-derived neutrophil-activating peptide 78) ELISA Kit; small inducible cytokine subfamily B; member 5 ELISA Kit; Small-inducible cytokine B5 ELISA Kit

Abbreviation

CXCL5

Uniprot No.

P42830

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

47 pg/mL-3000 pg/mL

Sensitivity

23.5 pg/mL

Assay Time

1-5h

Sample Loading Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human ENA-78/CXCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	99
	Range %	92-106
1:2	Average %	95
	Range %	88-102
1:4	Average %	89
	Range %	84-95
1:8	Average %	92
	Range %	85-99

Recovery

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml

OD1

OD2

Average

Corrected

3000

3.093

2.934

3.013

2.831

1500

2.440

2.567

2.504

2.322

750

1.797

1.828

1.813

1.630

375

1.248

1.312

1.280

1.098

187.5

0.604

0.621

0.613

0.430

0.386

0.373

0.380

0.198

0.292

0.284

0.288

0.106

0.179

0.185

0.182

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Description

This ELISA kit provides quantitative measurement of CXCL5 (ENA-78) in human samples, supporting research into neutrophil recruitment and CXC chemokine signaling in inflammatory and tumor microenvironments. Because CXCL5 levels can vary dramatically between healthy baseline and active inflammatory or tumorigenic states, the kit is designed to capture this biologically meaningful dynamic range without excessive sample manipulation.

Detection Range: The 47–3000 pg/mL range spans from low baseline circulating CXCL5 concentrations in healthy plasma to the elevated levels observed in sepsis, COPD exacerbations, and tumor-associated angiogenesis, minimizing the need for serial dilutions in most inflammatory disease samples.

Sensitivity: At 23.5 pg/mL, the kit detects CXCL5 in conditions where expression is modest, such as early-stage tumor stroma or resting endothelial cell supernatants, where neutrophil-recruiting chemokine gradients are just forming.

Sample Compatibility: Validation across serum, plasma, and tissue homogenates enables researchers to correlate systemic CXCL5 levels with local tissue expression — critical for studies dissecting neutrophil infiltration into tumor or inflamed tissue compartments.

Assay Efficiency: The 1–5 hour protocol using 50–100 µL accommodates limited clinical specimens, such as serial plasma draws from patients, and small tissue biopsies where homogenate volume is constrained.

This kit is well-suited for studies in tumor-associated neutrophil biology, chronic obstructive pulmonary disease, and obesity-linked adipose tissue inflammation.

Citations

Code	CSB-E08178h-IS
Size	96T,5×96T,10×96T
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.