Human Integrin beta-1(ITGB1) ELISA kit

Code CSB-EL011880HU
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P05556
Lead Time 5-7 working days
Abbreviation ITGB1
Protein Biological Process 1 Cell Adhesion
Target Name integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12)
Alias CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB, fibronectin receptor beta subunit|integrin VLA-4 beta subunit|integrin beta 1
Species Homo sapiens (Human)
Protein Biological Process 3 Cell adhesion
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Integrins are heterodimeric proteins made up of alpha and beta subunits. At least 18 alpha and 8 beta subunits have been described in mammals. Integrin family members are membrane receptors involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metastatic diffusion of tumor cells. This gene encodes a beta subunit. Multiple alternatively spliced transcript variants which encode different protein isoforms have been found for this gene.
HGNC 6153
RGD 2927
MGI 96610
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human ITGB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:5 Average % 93  
Range % 86-96  
1:10 Average % 89  
Range % 85-94  
1:20 Average % 88  
Range % 82-92  
1:40 Average % 102  
Range % 98-106  
Recovery
The recovery of human ITGB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 102 93-105  
EDTA plasma (n=4) 87 82-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
4000 2.361 2.469 2.415 2.284  
2000 2.015 1.924 1.970 1.839  
1000 1.332 1.356 1.344 1.213  
500 0.741 0.732 0.737 0.606  
250 0.402 0.413 0.408 0.277  
125 0.244 0.255 0.250 0.119  
62.5 0.181 0.188 0.185 0.054  
0 0.130 0.132 0.131    
References
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Function Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen. Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin. Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. Integrin alpha-5/beta-1 is a receptor for fibrinogen. Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin. Integrin alpha-4/beta-1 is a receptor for VCAM1. It recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G-I-E-L in cytotactin. Integrin alpha-3/beta-1 is a receptor for epiligrin, thrombospondin and CSPG4. Alpha-3/beta-1 may mediate with LGALS3 the stimulation by CSPG4 of endothelial cells migration. Integrin alpha-V/beta-1 is a receptor for vitronectin. Beta-1 integrins recognize the sequence R-G-D in a wide array of ligands. Isoform 2 interferes with isoform 1 resulting in a dominant negative effect on cell adhesion and migration (in vitro). When associated with alpha-7/beta-1 integrin, regulates cell adhesion and laminin matrix deposition. Involved in promoting endothelial cell motility and angiogenesis. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process and the formation of mineralized bone nodules. May be involved in up-regulation of the activity of kinases such as PKC via binding to KRT1. Together with KRT1 and RACK1, serves as a platform for SRC activation or inactivation. Plays a mechanistic adhesive role during telophase, required for the successful completion of cytokinesis. Integrin alpha-3/beta-1 provides a docking site for FAP (seprase) at invadopodia plasma membranes in a collagen-dependent manner and hence may participate in the adhesion, formation of invadopodia and matrix degradation processes, promoting cell invasion. ITGA4
Subcellular Location Cell membrane, Single-pass type I membrane protein, Cell projection, invadopodium membrane, Single-pass type I membrane protein, Cell projection, ruffle membrane, Single-pass type I membrane protein, Recycling endosome, Melanosome, Cleavage furrow, Cell projection, lamellipodium, Cell projection, ruffle, Cell junction, focal adhesion, Cell surface, Note=Isoform 2 does not localize to focal adhesions, Highly enriched in stage I melanosomes, Located on plasma membrane of neuroblastoma NMB7 cells, In a lung cancer cell line, in prometaphase and metaphase, localizes diffusely at the membrane and in a few intracellular vesicles, In early telophase, detected mainly on the matrix-facing side of the cells, By mid-telophase, concentrated to the ingressing cleavage furrow, mainly to the basal side of the furrow, In late telophase, concentrated to the extending protrusions formed at the opposite ends of the spreading daughter cells, in vesicles at the base of the lamellipodia formed by the separating daughter cells, Colocalizes with ITGB1BP1 and metastatic suppressor protein NME2 at the edge or peripheral ruffles and lamellipodia during the early stages of cell spreading on fibronectin or collagen, Translocates from peripheral focal adhesions sites to fibrillar adhesions in a ITGB1BP1-dependent manner, Enriched preferentially at invadopodia, cell membrane protrusions that correspond to sites of cell invasion, in a collagen-dependent manner, Localized at plasma and ruffle membranes in a collagen-independent manner, SUBCELLULAR LOCATION: Isoform 5: Cell membrane, sarcolemma, Cell junction
Protein Families Integrin beta chain family
Tissue Specificity Isoform 1 is widely expressed, other isoforms are generally coexpressed with a more restricted distribution. Isoform 2 is expressed in skin, liver, skeletal muscle, cardiac muscle, placenta, umbilical vein endothelial cells, neuroblastoma cells, lymphoma
Database Links

HGNC: 6153

OMIM: 135630

KEGG: hsa:3688

STRING: 9606.ENSP00000303351

UniGene: Hs.643813

Pathway PI3K-Akt signaling pathway
Focal adhesion
Regulation of actin cytoskeleton
Leukocyte transendothelial migration
Rap1 signaling pathway

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